JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST001025/files/B201123_09_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf11.rawhttps://storage.jpostdb.org/JPST001025/files/B201121_07_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf9-1.rawhttps://storage.jpostdb.org/JPST001025/files/B201121_06_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf8.rawhttps://storage.jpostdb.org/JPST001025/files/B201121_03_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf6.rawhttps://storage.jpostdb.org/JPST001025/files/B201121_05_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf7-2.rawhttps://storage.jpostdb.org/JPST001025/files/B201121_08_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf9-2.rawhttps://storage.jpostdb.org/JPST001025/files/B201121_04_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf7-1.rawhttps://storage.jpostdb.org/JPST001025/files/B201121_09_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf10.rawhttps://storage.jpostdb.org/JPST001025/files/B201121_02_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf5.rawhttps://storage.jpostdb.org/JPST001025/files/ODA-MS1_BS3_xiSEARCH_2LinkFDRBB.mzidhttps://storage.jpostdb.org/JPST001025/files/recal_B201121_07_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf9-1.mgfhttps://storage.jpostdb.org/JPST001025/files/recal_B201121_05_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf7-2.mgfhttps://storage.jpostdb.org/JPST001025/files/recal_B201121_02_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf5.mgfhttps://storage.jpostdb.org/JPST001025/files/recal_B201121_09_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf10.mgfhttps://storage.jpostdb.org/JPST001025/files/recal_B201121_06_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf8.mgfhttps://storage.jpostdb.org/JPST001025/files/recal_B201121_08_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf9-2.mgfhttps://storage.jpostdb.org/JPST001025/files/recal_B201121_04_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf7-1.mgfhttps://storage.jpostdb.org/JPST001025/files/recal_B201121_03_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf6.mgfhttps://storage.jpostdb.org/JPST001025/files/recal_B201123_09_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf11.mgfhttps://storage.jpostdb.org/JPST001025/files/ODA-MS1_26protein.fastaprimaryOK200ProteomicsJuri RappsilberTetrahymenahttps://repository.jpostdb.org/entry/JPST001025Technische Universität BerlinjPOSTThe main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate <i>Tetrahymena</i> to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.Shulin packages axonemal outer dynein arms for ciliary targeting.Mali Girish R GR, Ali Ferdos Abid FA, Lau Clinton K CK, Begum Farida F, Boulanger Jérôme J, Howe Jonathan D JD, Chen Zhuo A ZA, Rappsilber Juri J, Skehel Mark M, Carter Andrew P APMass Spectrum Analysis, Mass Spectrum, Analyses, determination, ODA, Spectrometry, Spectrum Analyses, Spectrum Analysis, Spectroscopy, CG16747, MS, Tetrahymenas, oda, chemical analysis, Mass, assay, Analysis, guf, DmelCG16747, AZ, l(2)02833, l(2)k09037., Mass Spectrum Analyses, Mass Spectroscopy, D-AZForms, Activity, conformation, Motor Activity, dhc64C, ODA, P62, Physical, Cilium, l(3)64Ca, sci, rhabdomyosarcoma, Locomotor, Protoplasm, Light Chain, Primary Cilia, Cdhc, Motor, Fs(3)Laborc, dhc64c, DmelCG7507, oda, motor activity, cDhc64C, symptoms, HOW, How, Dhc64c, Dynein Adenosinetriphosphatase, Dynein Light Intermediate Chains, guf, AZ, Upper Arm, l(3)j5D5, Arm, Dhc, DHC, Dynein Light Chain, 24B, Chain, axonemal motor activity, l(3)s2612, Motor Activities, ligand, l(2)k09037, Nodal Cilium, stru, Brachium, l(3)S053606, Locomotor Activity, Intermediate Chains, CG10293, dhc, Upper, CD, l(3)j5B5, cilium formation, ATPase, Adenosinetriphosphatase, cilium organization, Lab, DmelCG10293, Dynein Intermediate, Nodal, alveolar, relational structural quality, Intermediate Chain, l(2)02833, Dynein Light, D-AZ, Fs(3)Lab, dynein ATPase activity, screening, Upper Arms, 0904/17, Flagellum, findings, ARMS, Heavy Chains, clone 2.39, Dynein Heavy Chain, Cytoplasms, Dynein Light Chains, Dynein Intermediate Chains, Dhc46C, cilium biogenesis, Primary, Dynein Heavy Chains, Fs(3)Sz18, qkr, l(3)S090417, RMS2, Nodal Cilia, microtubule-based flagellum assembly, CG7507, kinetochore motor activity, rhabdomyosarcoma alveolar, Electron Microscopy, Arms, SZ1, Tetrahymenas, rhabdomyosarcoma 2, KH93F, Light Chains, Amplification Refractory Mutation System, Dynein, DHC64C, Heavy Chain, who, Dynein Adenosine Triphosphatase, Motile, Adenosine Triphosphatase, Chains, Dynein ATPase, cilium morphogenesis, Locomotor Activities, Tails, Motile Cilia, cDhc, Dhc64, signs, ciliogenesis, Who/How, FCP-A, Dynein Intermediate Chain, Cilia, Dynein Light Intermediate Chain, kinesin motor activity, Activities, CG16747, Physical Activities, Su(Gl)77, Brachiums, Protoplasms, Primary Cilium, HMW MAP, qkr[93F], anon-EST:Liang-2.39, Motile Cilium, Dynein Heavy, DmelCG16747, Dynein ATP phosphohydrolase (tubulin-translocating), Nodal Cilia.dynein ATPase activity, Upper Arms, ARMS, Heavy Chains, Dynein Heavy Chain, dhc64C, Dynein Light Chains, Dynein Intermediate Chains, Dhc46C, l(3)64Ca, Dynein Heavy Chains, Fs(3)Sz18, RMS2, rhabdomyosarcoma, CG7507, Light Chain, kinetochore motor activity, rhabdomyosarcoma alveolar, Cdhc, Arms, Fs(3)Laborc, dhc64c, DmelCG7507, rhabdomyosarcoma 2, cDhc64C, Light Chains, Amplification Refractory Mutation System, Dhc64c, Dynein, Dynein Adenosinetriphosphatase, Dynein Light Intermediate Chains, DHC64C, Heavy Chain, Upper Arm, Arm, Dhc, DHC, Dynein Light Chain, Dynein Adenosine Triphosphatase, Chain, Adenosine Triphosphatase, axonemal motor activity, Chains, Dynein ATPase, cDhc, Dhc64, Brachium, Intermediate Chains, FCP-A, Dynein Intermediate Chain, dhc, Dynein Light Intermediate Chain, kinesin motor activity, Upper, CD, ATPase, Adenosinetriphosphatase, Su(Gl)77, Brachiums, Lab, Dynein Intermediate, HMW MAP, alveolar., Dynein Heavy, Intermediate Chain, Dynein Light, Dynein ATP phosphohydrolase (tubulin-translocating), Fs(3)Labdhc64C, ODA, l(3)64Ca, Cilium, Spectrum Analyses, rhabdomyosarcoma, Light Chain, Primary Cilia, Cdhc, Fs(3)Laborc, dhc64c, DmelCG7507, oda, cDhc64C, Mass, Dhc64c, Analysis, Dynein Adenosinetriphosphatase, Dynein Light Intermediate Chains, guf, AZ, Upper Arm, Arm, Mass Spectroscopy, Dhc, DHC, Dynein Light Chain, Mass Spectrum Analysis, Chain, axonemal motor activity, Analyses, l(2)k09037, Nodal Cilium, Brachium, Intermediate Chains, dhc, Upper, CD, ATPase, Adenosinetriphosphatase, Lab, Dynein Intermediate, Nodal, alveolar, Intermediate Chain, l(2)02833, Dynein Light, D-AZ, Fs(3)Lab, dynein ATPase activity, Upper Arms, ARMS, Heavy Chains, Dynein Heavy Chain, Dynein Light Chains, Dynein Intermediate Chains, Dhc46C, Dynein Heavy Chains, Primary, Fs(3)Sz18, RMS2, Spectrum Analysis, Nodal Cilia, CG7507, Spectroscopy, kinetochore motor activity, rhabdomyosarcoma alveolar, MS, Arms, rhabdomyosarcoma 2, Light Chains, Amplification Refractory Mutation System, Dynein, DHC64C, Heavy Chain, Mass Spectrum Analyses, Dynein Adenosine Triphosphatase, Mass Spectrum, Adenosine Triphosphatase, Motile, Chains, Dynein ATPase, Motile Cilia, cDhc, Spectrometry, Dhc64, FCP-A, Dynein Intermediate Chain, Dynein Light Intermediate Chain, kinesin motor activity, Cilia, CG16747, ligand., Su(Gl)77, Brachiums, Primary Cilium, HMW MAP, Motile Cilium, Dynein Heavy, DmelCG16747, Dynein ATP phosphohydrolase (tubulin-translocating)falseCrosslinking/mass spectrometry (CLMS) analysis of Tetrahymena ODA-Q22MS1 complexThe outer dynein arms (ODAs) are the main force generators in cilia. Crosslinking/mass spectrometry (CLMS) was applied to investigate the interaction between ODA and its binding factor Q22MS1. Tue Dec 07 00:00:00 GMT 2021PXD022936589033632841