{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Txt":["https://storage.jpostdb.org/JPST001031/files/evidence.txt","https://storage.jpostdb.org/JPST001031/files/libraryMatch.txt"],"Raw":["https://storage.jpostdb.org/JPST001031/files/191014_C19_029_QHF1_PTM_Rev01_%2319_042_PAP300.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP261.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP259.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP265.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP254.raw","https://storage.jpostdb.org/JPST001031/files/191014_C19_029_QHF1_PTM_Rev01_%2319_042_PAP296.raw","https://storage.jpostdb.org/JPST001031/files/191014_C19_029_QHF1_PTM_Rev01_%2319_042_PAP298.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP262.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP256.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP260.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP264.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP258.raw","https://storage.jpostdb.org/JPST001031/files/191014_C19_029_QHF1_PTM_Rev01_%2319_042_PAP299.raw","https://storage.jpostdb.org/JPST001031/files/191014_C19_029_QHF1_PTM_Rev01_%2319_042_PAP295.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP255.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP257.raw","https://storage.jpostdb.org/JPST001031/files/190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP263.raw","https://storage.jpostdb.org/JPST001031/files/191014_C19_029_QHF1_PTM_Rev01_%2319_042_PAP297.raw"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Philip Kaenel"],"species":["Homo Sapiens (human)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST001031"],"submitter_affiliation":["University of Münster - WWU - Institute for Biology and Biotechnology of Plants"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"pubmed_abstract":["Proteostasis reflects the well-balanced synthesis, trafficking and degradation of cellular proteins. This is a fundamental aspect of the dynamic cellular proteome, which integrates multiple signaling pathways, but it becomes increasingly error-prone during aging. Phosphatidylethanolamine-binding proteins (PEBPs) are highly conserved regulators of signaling networks and could therefore affect aging-related processes. To test this hypothesis, we expressed PEPBs in a heterologous context to determine their ectopic activity. We found that heterologous expression of the tobacco (<i>Nicotiana tabacum</i>) PEBP NtFT4 in <i>Drosophila melanogaster</i> significantly increased the lifespan of adult flies and reduced age-related locomotor decline. Similarly, overexpression of the Drosophila ortholog CG7054 increased longevity, whereas its suppression by RNA interference had the opposite effect. In tobacco, NtFT4 acts as a floral regulator by integrating environmental and intrinsic stimuli to promote the transition to reproductive growth. In Drosophila, NtFT4 engaged distinct targets related to proteostasis, such as HSP26. In older flies, it also prolonged <i>Hsp26</i> gene expression, which promotes longevity by maintaining protein integrity. In NtFT4-transgenic flies, we identified deregulated genes encoding proteases that may contribute to proteome stability at equilibrium. Our results demonstrate that the expression of NtFT4 influences multiple aspects of the proteome maintenance system via both physical interactions and transcriptional regulation, potentially explaining the aging-related phenotypes we observed."],"pubmed_title":["The tobacco phosphatidylethanolamine-binding protein NtFT4 increases the lifespan of <i>Drosophila melanogaster</i> by interacting with the proteostasis network."],"pubmed_authors":["Känel Philip P, Noll Gundula A GA, Schroedter Katrin K, Naffin Elke E, Kronenberg Julia J, Busswinkel Franziska F, Twyman Richard M RM, Klämbt Christian C, Prüfer Dirk D"],"pubmed_title_synonyms":["Life Span, fruit fly, lifespan, Lifespans, Life Spans, Homeostasis, entire lifespan, entire life cycle, Length of Life, life, Protein Homeostases, Nicotiana tabacum var. Samsun, Protein Homeostasis, Homeostases, Raf Kinase Inhibitor Protein, Sophophora melanogaster, tobacco, melanogaster, D. melanogaster, Drosophila, Hippocampal Cholinergic Neurostimulating Peptide Precursor, Phosphatidylethanolamine-Binding Protein, Drosophila melangaster, Nicotania tabacum, common tobacco, Drosophila melanogasters, Protein, Proteostases., HCNP Precursor Protein, American tobacco, Lifespan"],"description_synonyms":["Eph-like tyrosine kinase 1, protein translation, d230, 293tsA1609neo, Activity, postnatal development, number, Gene, ETK1, dTAFII250, growth and development, protein, B-cell receptor complex, protein-containing complex, EfW1, supernumerary, Migrant Workers, Cell Growth in Number, 293T, protrusion, Productivity, Human, dmTAF[[II]]230, antibody, Human cell, Tier, BCR complex, HEK4, dmTAF1, Taf230, Gene Products, Line, Cell Number Growth, Animal, Number Growth, protein aggregate, Human embryo kinase, immunoglobulin complex, TYRO4, Tyrosine-protein kinase TYRO4, RCB2202, TAF250, Animalia, HEK293T, increased, Taf200, doubled, Growth, anatomical systems, dTAF[[II]]250, Migrant Worker, 293-T, TFIID TAF250, cel, cell, membrane bound, non-developmental growth of a unicellular organism, Transients, EK4, Nonmigrant, General activity., Nonmigrants, Taf1p, metabolic process resulting in cell growth, metazoa, Worker, Transient, free, Squatter, Hek 293T, dTAF250, cell proliferation, 2.7.10.1, HEK-293T, Suspension, Squatters, TAF, B-lymphocyte receptor complex, Transfections, ETK, Multiplication, Human Embryonic Kidney 293T, protein anabolism, dTAF[[II]]230, TAF[[II]]250, anatomical protrusion, Cellular Proliferation, protein biosynthetic process, protein complex, body, animalia, metabolism resulting in cell growth, Proteins, Cell Lines, TAF200, Nicotiana tabacum var. Samsun, HEK 293 T, l(3)84Ab, whole body, BG:DS00004.13, TAFII-250, tobacco, TAF250/230, antibodies, Cell, dTAF230, development, cell expansion, TAFII250, transfect, native protein, p230, Migrant, non-developmental cell growth, Nicotania tabacum, Protein, Metazoa, Nomad, EPH-like kinase 4, protein formation, TAF[[II]]250/230, Cellular, TFIID, protein biosynthesis, Workers, American tobacco, NICR1066, Migrants and Transients, Lines, opsonin activity, Taf[[II]]250, TAF[[II]]230, whole organism, growth pattern, Engineerings, increased number, non-developmental growth, postnatal growth, hEK4, TAF[II]250, CG17603, 293 T, TAF[[II]], Protein Gene Products, Gene Proteins, present in greater numbers in organism, immunoglobulin, DmelCG17603, B cell receptor accessory molecule complex, Taf250, protein synthesis, spine, Proliferation, SR3-5, Human 细胞, Nomads, common tobacco, Cell Multiplication, Koerper, cardinality, B lymphocyte receptor complex, HEK, Cell Number, B cell receptor activity, Migrants, Tyrosine-protein kinase receptor ETK1, growth, HEK-293-T, TAF230, accessory, TAF1"],"name_synonyms":["Human, Protein Gene Products, Gene Proteins, human being, Man (Taxonomy), Homo sapiens, Modern Man, Nicotania tabacum, Protein, Modern, Gene Products, Proteins, Nicotiana tabacum var. Samsun, Gene, American tobacco, tobacco, common tobacco., Man, human"],"pubmed_abstract_synonyms":["biochemical pathways, subgenus>, fruit fly, Materials, Activity, HCNPpp, adult stage, postnatal development, growth and development, protein, Aging, melanogaster, Social Controls, hsp28, hsp27, hsp26, Biological, Alleviating interaction, Post Transcriptional, AA959943, 1, cellular degradation, protein aggregate, Formal Social Controls, adult, Flies, multicellular organismal biosynthetic process, Cephalin, Senescence, increased, single-organism biosynthetic process, 26, Life Span, 27, 28, Gene Expressions, catabolism, Moods, entire life cycle, hsp-27, Length of Life, Drosophilas, Fly, hypoplasia, Homeostases, quelling, Acts, DmelCG4183, Social, Biological Aging, Gene Silencings, reaction, biotransformation, TG, fruit flies, hsp27/26, single organism signaling, hsp 27, Acta-2, hsp 26, anatomical protrusion, Lifespans, 26K, Actsk-1, tobacco, results, PBP, mislocalized, 27K, RENBP, Genetic Materials, secretion, RNA Silencing, regulator, Adults, Genetic Material, Post-Transcriptional Gene, 1823, Drosophila <basidiomycete fungi>, Hsp 27, True Flies, length of life, CG4183, growth pattern, non-developmental growth, life, Plant, fruit fly <Drosophila>, Control, Controls, Co Suppression, small hsp locus 67B, Post Transcriptional Gene Silencing, AGE, phosphatidylethanolamines, Drosophila, Gene Silencing, spine, Material, common tobacco, Drosophila Fruit Fly, Cistron, Co-Suppression, Regulation, Proteomes, accessory, Drosophila <flies, Drosophila Fruit Flies, Regulations, post-transcriptional gene silencing by RNA, RKIP, lifespan, Drosophila Fallen, ectopic, Protein Homeostasis, Gene, Sophophora melanogaster, protein-containing complex, supernumerary, cellular catabolism, protrusion, PEBP, reduced, Plants, Drosophila melangaster, Gene Products, HEL-210, Tobacco, Mood, tiny, O-(1-beta-acyl-2-acyl-sn-glycero-3-phospho)-ethanolamine, DmelCG4466, anatomical systems, Genetic, breakdown of chemical, entire lifespan, ligand, True Fly, Maintenances, suppressive genetic interaction (sensu inequality), Cosuppression, Expressions, Proteostases, DmelCG7054, Drosophila Fruit, Dipteras, Expression, Tobacco Plants, O-(1-beta-Acyl-2-acyl-sn-glycero-3-phospho)ethanolamine, Lifespan, small, flies, cellular breakdown, RNA, Hsp28, PEBP-1, Life Spans, True, degradation, 2-diacyl-sn-glycero-3-phosphoethanolamine, HEL-S-34, Formal Social Control, RnBP, protein complex, synthesize, Affects, Protein Homeostases, Proteins, posttranscriptional gene silencing by siRNA, Phenotypes., total expressed protein, Nicotiana tabacum var. Samsun, GlcNAc 2-epimerase, anon-WO0140519.69, genus>, cosuppression, Cistrons, (3-Phosphatidyl)-ethanolamine, D. melanogaster, RNAi, development, N-acetyl-D-glucosamine 2-epimerase, Interference, native protein, Social Control, PtdEtn, Nicotania tabacum, Protein, HSP26, HSP28, HSP27, American tobacco, Silencing, breakdown of molecule, Post-Transcriptional Gene Silencings, Homeostasis, biodegradation, distinct, underdeveloped, Nicotiana tabacum, Posttranscriptional, increased number, Posttranscriptional Gene Silencing, postnatal growth, (3-Phosphatidyl)ethanolamine, Tobacco Plant, HCNP, Fruit Flies, Protein Gene Products, Gene Proteins, present in greater numbers in organism, breakdown of substance, DmHSP27, Post-Transcriptional, signalling process, Drosophila melanogasters, renin-binding protein, CG4466, Fruit Fly, DmHsp26, regulation, PE, DmHsp27, 1-Acyl-2-acyl-sn-glycero-3-phosphoethanolamine, Post-Transcriptional Gene Silencing, growth, General activity, hypothesis, Dhsp27"],"additional_accession":[]},"is_claimable":false,"name":"Co-immunoprecipitated human proteins with tobacco NtFT4","description":"Biopharmaceutical proteins are produced in mammalian cells by transient expression or stable transformation, but both processes require robust and viable cells. Cell line engineering must therefore balance improved cell growth and viability with high productivity. We tested the expression of non-mammalian phosphatidylethanolamine binding proteins to enhance cell proliferation. The tobacco protein NtFT4 improved the proliferation and viability of HEK-293T cells in animal component-free suspension cultures. Viable cell density is usually impaired by efficient transfection, but we found that the number of HEK-293TNtFT4 cells doubled at the peak of protein expression and the antibody yield increased by approximately one third. The improved growth and viability were observed in different cell backgrounds, suggesting the system is transferable. Furthermore, high densities of viable HEK-293TNtFT4 cells will allow further modifications to boost protein synthesis. The established HEK-293TNtFT4 cell line provides a new human cell platform that increases cell proliferation without sacrificing translational activity.","dates":{"publication":"Fri Dec 10 00:00:00 GMT 2021"},"accession":"PXD023020","cross_references":{"TAXONOMY":["9606"],"pubmed":["35396341"]}}