{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Raw":["https://storage.jpostdb.org/JPST001108/files/S.raw","https://storage.jpostdb.org/JPST001108/files/T.raw"],"Mzid":["https://storage.jpostdb.org/JPST001108/files/T.mzid","https://storage.jpostdb.org/JPST001108/files/S.mzid"],"Mgf":["https://storage.jpostdb.org/JPST001108/files/T.mgf","https://storage.jpostdb.org/JPST001108/files/S.mgf"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Genki Horiguchi"],"species":["Cellular Organisms"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST001108"],"submitter_affiliation":["Toyo University"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"pubmed_abstract":["Hygrophila polysperma is a heterophyllous amphibious plant. The growth of H. polysperma in submerged conditions is challenging due to the low CO2 environment, increased resistance to gas diffusion, and bicarbonate ion (HCO3 -) being the dominant dissolved inorganic carbon source. The submerged leaves of H. polysperma have significantly higher rates of underwater photosynthesis compared with the terrestrial leaves. 4,4'-Diisothiocyanatostilbene-2,2'-disulfonate (DIDS), an anion exchanger protein inhibitor, and ethoxyzolamide (EZ), an inhibitor of internal carbonic anhydrase, repressed underwater photosynthesis by the submerged leaves. These results suggested that H. polysperma acclimates to the submerged condition by using HCO3 - for photosynthesis. H. polysperma transports HCO3 - into the leaf by a DIDS-sensitive HCO3 - transporter and converted to CO2 by carbonic anhydrase. Additionally, proteome analysis revealed that submerged leaves accumulated fewer proteins associated with C4 photosynthesis compared with terrestrial leaves. This finding suggested that H. polysperma is capable of C4 and C3 photosynthesis in the terrestrial and submerged leaves, respectively. The ratio of phosphoenol pyruvate carboxylase to ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) in the submerged leaves was less than that in the terrestrial leaves. Upon anatomical observation, the terrestrial leaves exhibited a phenotype similar to the Kranz anatomy found among C4 plants; however, chloroplasts in the bundle sheath cells were not located adjacent to the vascular bundles, and the typical Kranz anatomy was absent in submerged leaves. These results suggest that H. polysperma performs proto-Kranz type photosynthesis in a terrestrial environment and shifts from a proto-Kranz type in terrestrial leaves to a HCO3 - use photosynthesis in the submerged environments."],"pubmed_title":["Transition From Proto-Kranz-Type Photosynthesis to HCO3 - Use Photosynthesis in the Amphibious Plant Hygrophila polysperma."],"pubmed_authors":["Horiguchi Genki G, Matsumoto Kaori K, Nemoto Kyosuke K, Inokuchi Mayu M, Hirotsu Naoki N"],"pubmed_title_synonyms":["AT1G11140, Photosynthetic, FON1, Pflanze, Photosynthesis Dark Reaction, STRUBBELIG, A4, Reductive Pentose Phosphate Cycle, SUPERMAN, Calvin Benson Cycle, Synaptosomal-associated 25 kDa protein, FLORAL ORGAN NUMBER 1, TYPE, Photosynthetic Carbon Fixation, LGMD2C, CG12298, viridiplantae, DAGA4, STRUBBELIG-RECEPTOR FAMILY 9, SUB, DmelCG12298, Photosynthesis Dark Reactions, Cycle, SUP, GENA70, Calvin-Benson Cycle, SCRAMBLED, MAM, SCG3, Carbon Fixation, KIF20A, plantae, DMDA1, mei-1794, Dub, Bdr, Plant, Miramar-weed, Calvin Benson Bassham Cycle, FLO10, Indian swampweed., Calvin-Benson-Bassham, Super protein, HERP, sp, SNAP-25, reaction, Mif1, DMDA, Calvin Cycle, SCARMD2, Calvin-Benson-Bassham Cycle, Calvin, SRF9, Dark Reactions of Photosynthesis, Calvin-Benson, T19D16.8, SCM, FLORAL DEFECTIVE 10"],"pubmed_abstract_synonyms":["(erythro-D)-isomer, ion, determination, increased resistance, FON1, Pflanze, postnatal development, A4, growth and development, protein, FLORAL ORGAN NUMBER 1, Photosynthetic Carbon Fixation, viridiplantae, Photosynthetic Carbon Fixation., countertransporter activity, SUB, solute:solute exchange, protein polypeptide chains, exact), diseases, DmelCG12298, Carbon-12, SUP, Impacts, SCRAMBLED, diseases and disorders, germacrene A synthase activity, Environmental Impacts, exchanger, protein aggregate, Carbon Fixation, oxidoreductase activity, KIF20A, (erythro-L)-isomer, C, miscellaneous, aniones, me75, human disease, ribulose, Bdr, acting on single donors with incorporation of molecular oxygen, proteins, plants, Super protein, Calvin-Benson-Bassham, Diffusions, D17Mit170, T1, sp, SNAP-25, Chloroplast, PA1, allergic reaction, Environmental Impact, Carbon, C16orf53, Calvin, Homo sapiens disease, associated, accumulated, ratio, (+)-germacrene A synthase activity, close to, exchange transporter activity, land plants, STRUBBELIG, Calvin Benson Cycle, Carboxylase, Tl3, GAST, Tl2, results, Anionen, Vitreous, Ionen, DL-erythro-pentulose, GENA70, GAS, 6-trans-farnesyl-diphosphate diphosphate-lyase (germacrene-A-forming) activity, absence, growth pattern, non-developmental growth, 6C, proportionality, Miramar-weed, Plant, Calvin Benson Bassham Cycle, rate, gas, solute:solute antiporter activity, 葉 (Japanese, anions, Phenotypes, disease, high resistance to, Vitreous Carbon, higher plants, DMDA, Pyruvate, carbonium, hypersensitivity reaction disease, Proteomes, FLORAL DEFECTIVE 10, AT1G11140, other disease, 2-trans, Gene, SUPERMAN, carbon, Reductive Pentose Phosphate Cycle, protein-containing complex, TYPE, CG12298, 維管束鞘 (Japanese, vaina del haz (Spanish, DAGA4, polypeptide chain, sensitive, Photosynthesis Dark Reactions, Cycle, Indian swampweed, Gene Products, anion, disease or disorder, Low, MAM, SCG3, sensitivity, plantae, oxygenase, proportion, iones, mei-1794, absent from organism, inhibiteur, ions, ion antiporter activity, Anion, Etioplast, non-neoplastic, Mif1, Kohlenstoff, Anatomies, inhibidor, Calvin-Benson-Bassham Cycle, disorder, SRF9, hypersensitivity reaction, Dark Reactions of Photosynthesis, inhibitors, cou, protein complex, Photosynthetic, 6-trans-farnesyl-diphosphate diphosphate-lyase [(+)-germacrene-A-forming] activity, Calvin cycle, Proteins, Photosynthesis Dark Reaction, disorders, total expressed protein, inhibitor, Synaptosomal-associated 25 kDa protein, medical condition, Etioplasts, (+)-(10R)-germacrene A synthase activity, carbone, Cell, porter, LGMD2C, Impact, STRUBBELIG-RECEPTOR FAMILY 9, carbono, near to, development, Environmental, Lr, native protein, natural protein, Protein, chemical analysis, Oxygenase, condition, Calvin-Benson Cycle, Pyruvate:carbon-dioxide ligase (ADP-forming), hypersensitivity, hypersensitive, DMDA1, antiport, Dub, postnatal growth, FLO10, HERP, C3 photosynthesis, hoja (Spanish, Carbon 12, Protein Gene Products, Gene Proteins, Ion, Calvin Cycle, approaches, vicinity of, SCARMD2, Bra, Environments, quotient, assay, Calvin-Benson, growth, T19D16.8, SCM"],"name_synonyms":["plantae, disease, other disease, human disease, exact), diseases, Pflanze, disease or disorder, disorders, Plant, condition, disorder, Homo sapiens disease, diseases and disorders, medical condition, hoja (Spanish, 葉 (Japanese, viridiplantae, non-neoplastic."],"description_synonyms":["disease, other disease, human disease, Leave, diseases, determination, Peptidomics, Plant Leave, chemical analysis, Leaves, disease or disorder, disorders, Plant, condition, disorder, Homo sapiens disease, diseases and disorders, assay, medical condition, Plant Leaf, Leaf, non-neoplastic."],"additional_accession":[]},"is_claimable":false,"name":"Leaf of amphibious plant developed under terrestrial or submerged condition","description":"Proteomics analysis of amphibious plant leaves. Leaves developed under terrestrial or submerged condition were used.","dates":{"publication":"Tue Jun 15 00:00:00 BST 2021"},"accession":"PXD024779","cross_references":{"TAXONOMY":["131567"],"pubmed":["34220895"]}}