JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST001103/files/G_ECL01372-2.rawhttps://storage.jpostdb.org/JPST001103/files/N_ECL01417.rawhttps://storage.jpostdb.org/JPST001103/files/I_ECL01414.rawhttps://storage.jpostdb.org/JPST001103/files/O_ECL01376-2.rawhttps://storage.jpostdb.org/JPST001103/files/L_ECL01374-2.rawhttps://storage.jpostdb.org/JPST001103/files/D_ECL01369-2.rawhttps://storage.jpostdb.org/JPST001103/files/H_ECL01373-2.rawhttps://storage.jpostdb.org/JPST001103/files/P_ECL01377-3.rawhttps://storage.jpostdb.org/JPST001103/files/M_ECL01416.rawhttps://storage.jpostdb.org/JPST001103/files/C_ECL01368-2.rawhttps://storage.jpostdb.org/JPST001103/files/E_ECL01370-2.rawhttps://storage.jpostdb.org/JPST001103/files/A_ECL00924.rawhttps://storage.jpostdb.org/JPST001103/files/J_ECL01415.rawhttps://storage.jpostdb.org/JPST001103/files/B_ECL01367-2.rawhttps://storage.jpostdb.org/JPST001103/files/K_ECL01375-2.rawhttps://storage.jpostdb.org/JPST001103/files/F_ECL01371-2.rawhttps://storage.jpostdb.org/JPST001103/files/K_ECL01375.rawhttps://storage.jpostdb.org/JPST001103/files/C_ECL01368-2.mgfhttps://storage.jpostdb.org/JPST001103/files/A_ECL00924.mgfhttps://storage.jpostdb.org/JPST001103/files/B_ECL01367-2.mgfprimaryOK200ProteomicsEmilio Gutierrez-BeltranArabidopsis Thaliana (mouse-ear Cress)https://repository.jpostdb.org/entry/JPST001103University of SevillajPOSTisolation and purification, purification, isolation.2610315D21Rik, land plants., 2410041A17Rik, determination, ACTG, ACTE, GFP cell, REHF-1, protein, fs(1)M34, Organelle, csp2, Act5, Protoplasm, l(1)G0420, DmelCG12051, tudor staphylococcus/micrococcal nuclease, Roles, BCLF-1, SND1, HEL-176, Concepts, CG4601, RAFT1, protein aggregate, Arabidopsis thaliana TUDOR-SN protein 2, Arabidopsis thaliana TUDOR-SN protein 1, imprinted and ancient gene protein, A, C, TSN2, TSN1, cyt5C, TUDOR-SN protein 1, TUDOR-SN protein 2, DHO, TBRBP, C3PO, MAC9.14, CG18572, tudor-SN, cell sheath, plants, purification, TUDOR-SN, isolation and purification, set, act 42A, Role Concepts, MAC9_14, Ac5C, CG4027, TSPAN-2, ACT, NET3, Act, NICR1047, l(1)G0330, Actin/BAP47, CTE-II, Cytoplasms, AACT, land plants, lamina, CTE-IIa, act, Ach1, hBACH, ACH1, LACH1, act42A, component 3 of promoter of RISC, GIG25, Role Concept, Su(b), Alpha-1-antichymotrypsin His-Pro-less, AFFX-Dros-ACTIN_M_r_at, GIG24, Role, DFNA26, actin, shell, LACH, GAT, DFNA20, RAPT1, bclf-1, AU040286, DmelCG4027, l(1)G0117, FRAPs (Fluorescence Recovery After Photobleaching), Plant, Actin, beta-actin/Bap47, l(1)G0486, l(1)G0245, actin5C, higher plants, l(1)G0009, ACTL3, Lach1, E430016J11Rik, BACH, Proteomes, Gruppe, l(1)Ab, Act5c, l(1)G0010, conformation, TRSLN, ACT5C, Gene, CG12051, Serpin A3, protein-containing complex, Bach, Ximpact, mug90, l(1)G0025, isolation, Gene Products, GFP 细胞, Cell growth-inhibiting gene 24|25 protein, T2I1.60, study, act5C, T2I1_60, layer, BRWS2, TB-RBP, Fluorescence Photobleaching Recovery, GFP, CG7008, TSN-1, flat, rchf1, fs(1)829, Act42a, anon-EST:fe2D2, 1700027G07Rik, grupos, DmelCG18572, trsln, T11, Frap1, relational structural quality, Act-5C, Cte-II, l(1)G0177, RCHF1, tbrbp, 42A, CPS, grupo, AtTudor1, AtTudor2, protein complex, Proteins, dJ393D12.2, total expressed protein, FRAP1, FRAP2, rehf-1, impact-a, group, Concept, dP100, CAD, native protein, beta-actin, Protein, TSN, chemical analysis, M32055, FRAP (Fluorescence Recovery After Photobleaching), core, Act42, p100, IMPACT, imprinted and ancient gene protein homolog, ACTA3, l(1)G0079, ensemble, PYR1, tsn, VSCM, Rest, act 5C, AI327068, FRAP, Protein Gene Products, Gene Proteins, 2610034C24Rik, DmelCG7008, DEL, Protoplasms, sheath of cells, layer of cells, DRORUD, BAP47, ACTSG, assay, groupe, Bap47, RWDD5, Actin5CfalseIsolation of RBP47 interactomeStress granules are dynamic structures, in which components belonging to the shell structure can exchange rapidly while components in the core layer may be less dynamic. The last group of proteins has been suggested to act as a scaffold to which other SG components are recruited. In a previous study, we observed that TSN does not exchange between the cytoplasm and foci upon a fluorescence recovery after photobleaching (FRAP) analysis, suggesting a role as a scaffold protein. Deletion of scaffold-like molecules has been suggest to have a high impact in membraneles organelles composition. Accordingly, and to better understand the role of TSN as a platform for SG components in plants, we characterized the SG proteome by isolation of GFP-tagged RBP47 protein from WT and double tsn1 tsn2 knock-out expressing plants.Fri Jun 11 00:00:00 BST 2021PXD0249623702