JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_4h_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_AnExSP_18h_2.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_SP3_18h_2.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Tetramer_IGD_1.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Tetramer_PEPPI-AnExSP_2.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Dimer_PEPPI-AnExSP_2.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Dimer_IGD_2.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_AnExSP_18h_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_18h_2.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_4h_2.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_AnExSP_18h_3.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Dimer_IGD_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_SP3_18h_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_SP3_18h_3.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_AnExSP_4h_3.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Tetramer_PEPPI-AnExSP_1.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Tetramer_IGD_2.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Dimer_PEPPI-AnExSP_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_18h_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_4h_3.rawprimaryOK200ProteomicsNobuaki TakemoriHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST001370Kazusa DNA Research InstitutejPOSTWe introduce a simple single-column protein digestion method for low-microgram-level samples containing sodium dodecyl sulfate and Coomassie dye that can be completed within a few hours.Bottom-up/cross-linking mass spectrometry <i>via</i> simplified sample processing on anion-exchange solid-phase extraction spin column.Takemori Ayako A, Kawashima Yusuke Y, Takemori Nobuaki NMass Spectrum Analysis, Mass Spectrum, aniones, single-organism developmental process, Analyses, postnatal development, postnatal growth, discus, Spectrometry, growth and development, Spectrum Analyses, study protocol, CG9908, Anion, Spectrum Analysis, sample population, anions, plan specification, Anionen, Spectroscopy, development, method, MS, DISC protein complex, DmelCG9908, sample, method used in an experiment, Mass, Mass., anion, disk, death receptor-induced signalling complex, death-inducing signalling complex, Disco, DISC, death receptor-induced signaling complex, Analysis, growth, disc, Mass Spectrum Analyses, Mass SpectroscopySulfuric acid monododecyl ester sodium salt, Dyes, teinture, Degradations, Sodium Dodecyl, An4, cou, Organic Pigment, Tissue Stain., Procedures, Sodium Lauryl, Degradation, Dodecyl Sulfate, ug, Pigments, Procedure, Protein Digestion, Tl3, Tl2, Digestions, Agent, Sodium Lauryl Sulfate, method, Techniques, Lr, Organic, Proteolyses, colorantes, Digestion, Method, PIG7, Organic Pigments, dyes, method used in an experiment, Protein, Studies, Pigment, Sodium, Low, Technique, Coloring Agent, Inorganic Pigments, me75, Tissue Stains, Protein Digestions, Dye, Tissue, µg, Lauryl Sulfate, Methodological, Farbstoff, colorante, Protein Degradations, Methodological Study, Protein Degradation, D17Mit170, Stains, T1, plan specification, Coloring, Study, xnup93, Irium, Stain, column, Methodological Studies, Bra, dye, SIMPLE, Sulfate, Inorganic, teintures, Farbstoffe, TP53I7Mass Spectrum Analysis, Spectroscopy, Anionen, Mass Spectrum, aniones, MS, Analyses, sample, Mass, anion, Spectrometry, Analysis, Spectrum Analyses, anions., Mass Spectrum Analyses, Spectrum Analysis, Anion, Mass Spectroscopy, sample populationhuman being, protein complex, Trypsin/K, Modern, Proteins, Gene, protein, Spectrum Analyses, protein-containing complex, Spectrum Analysis, Human, Anionen, Spectroscopy, method, MS, DISC protein complex, native protein, Homo sapiens, method used in an experiment, Protein, Gene Products, Mass, anion, DGS, disk, death-inducing signalling complex, Disco, DISC, Analysis, protein aggregate, disc, Man, Mass Spectrum Analyses, Mass Spectroscopy, Mass Spectrum Analysis, Mass Spectrum, aniones, PRSS, Man (Taxonomy), Analyses, gel, discus, beta-Trypsin, Tripcellim, Spectrometry, study protocol, CG9908, beta Trypsin, Anion, anions, DIA, human, CG1106, plan specification, Protein Gene Products, Gene Proteins, Trypure, DmelCG9908, DmelCG1106, Modern Man, Mass., death receptor-induced signalling complex, death receptor-induced signaling complexfalseDevelopment of sequential sample processing protocol on a single anion-exchange solid-phase extraction disc for bottom-up/cross-linking mass spectrometryIn this project, we developed a rapid enzymatic digestion protocol within an anion exchange StageTip named AnExSP (anion-exchange disc-assisted sequential sample preparation) and, using data-independent acquisition, analyzed its digestion performance of human cellular protein extracts (HCPE) using trypsin and trypsin/Lys-C. The outstanding effectiveness and simplicity of AnExSP in sample preparation for gel-based cross-linking mass spectrometry was also verified.Wed Apr 20 00:00:00 BST 2022PXD029444960634897310