JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_4h_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_AnExSP_18h_2.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_SP3_18h_2.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Tetramer_IGD_1.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Tetramer_PEPPI-AnExSP_2.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Dimer_PEPPI-AnExSP_2.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Dimer_IGD_2.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_AnExSP_18h_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_18h_2.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_4h_2.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_AnExSP_18h_3.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Dimer_IGD_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_SP3_18h_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_SP3_18h_3.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-1ug_AnExSP_4h_3.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Tetramer_PEPPI-AnExSP_1.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Tetramer_IGD_2.rawhttps://storage.jpostdb.org/JPST001370/files/XL-MS_Hemoglobin_Dimer_PEPPI-AnExSP_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_18h_1.rawhttps://storage.jpostdb.org/JPST001370/files/DIA_HCPE-10ug_AnExSP_4h_3.rawprimaryOK200ProteomicsNobuaki TakemoriHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST001370Kazusa DNA Research InstitutejPOSTWe introduce a simple single-column protein digestion method for low-microgram-level samples containing sodium dodecyl sulfate and Coomassie dye that can be completed within a few hours.Bottom-up/cross-linking mass spectrometry <i>via</i> simplified sample processing on anion-exchange solid-phase extraction spin column.Takemori Ayako A, Kawashima Yusuke Y, Takemori Nobuaki NMass Spectrum Analysis, Spectroscopy, Anionen, Mass Spectrum, aniones, Analyses, sample, solid., Mass, anion, Spectrometry, Analysis, Spectrum Analyses, Mass Spectrum Analyses, Spectrum Analysis, Anion, Mass Spectroscopy, sample population, anionsSulfuric acid monododecyl ester sodium salt, me75, Degradations, Sodium Dodecyl, cou, Protein Digestions, Sodium Lauryl, Degradation, Dodecyl Sulfate, ug, µg, Lauryl Sulfate, An4., Protein Degradations, Protein Digestion, Tl3, Tl2, Digestions, Protein Degradation, D17Mit170, T1, plan specification, xnup93, Irium, Sodium Lauryl Sulfate, method, Lr, column, Proteolyses, Digestion, PIG7, method used in an experiment, Protein, Bra, dye, Sodium, Low, SIMPLE, Sulfate, TP53I7Mass Spectrum Analysis, Mass Spectrum, aniones, single-organism developmental process, Analyses, discus, Spectrometry, Spectrum Analyses, study protocol, CG9908, Anion, Spectrum Analysis, sample population, anions, plan specification, Anionen, Spectroscopy, development, solid, method, DISC protein complex, DmelCG9908, sample, method used in an experiment, Mass, Mass., anion, disk, death receptor-induced signalling complex, death-inducing signalling complex, Disco, DISC, death receptor-induced signaling complex, Analysis, disc, Mass Spectrum Analyses, Mass Spectroscopyhuman being, protein complex, Trypsin/K, protein, Spectrum Analyses, protein-containing complex, Spectrum Analysis, Anionen, Spectroscopy, method, protein polypeptide chains, DISC protein complex, native protein, natural protein, polypeptide chain, method used in an experiment, Protein, Mass, anion, DGS, disk, death-inducing signalling complex, Disco, DISC, Analysis, protein aggregate, disc, Mass Spectrum Analyses, Mass Spectroscopy, Mass Spectrum Analysis, Mass Spectrum, aniones, Analyses, gel, discus, beta-Trypsin, Tripcellim, Spectrometry, proteins, study protocol, CG9908, man, beta Trypsin, Anion, anions, DIA, human, CG1106, plan specification, Trypure, DmelCG9908, DmelCG1106, Mass., death receptor-induced signalling complex, death receptor-induced signaling complexfalseDevelopment of sequential sample processing protocol on a single anion-exchange solid-phase extraction disc for bottom-up/cross-linking mass spectrometryIn this project, we developed a rapid enzymatic digestion protocol within an anion exchange StageTip named AnExSP (anion-exchange disc-assisted sequential sample preparation) and, using data-independent acquisition, analyzed its digestion performance of human cellular protein extracts (HCPE) using trypsin and trypsin/Lys-C. The outstanding effectiveness and simplicity of AnExSP in sample preparation for gel-based cross-linking mass spectrometry was also verified.Wed Apr 20 00:00:00 BST 2022PXD029444960634897310