JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%233-1.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%231-2.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%232-4.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%232-2.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%231-6.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%233-5.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%234-1.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%231-5.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%232-5.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%231-3.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%233-2.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%234-2.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%232-3.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%233-4.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%231-1.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%232-1.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%233-6.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%233-3.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%231-4.rawhttps://storage.jpostdb.org/JPST001493/files/orb_190624_AE-MF-7_%232-6.rawprimaryOK200ProteomicsNakai AkiraHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST001493Department of Biochemistry and Molecular Biology, Graduate School of Medicine, Yamaguchi UniversityjPOSTl(2)EMS34-12, CG33554, Shock, Temperature, TRRAP, NipA, Circulatory, Proteins, Nipped[A], l(2)41Ah, DmelCG33554, Gene, Nip-A, proteins, tra1, Nipped A, TR-AP, dTra1, 41Ah, Failure, Protein Gene Products, Hypovolemic Shock, Circulatory Collapse, Collapse, Circulatory Failure., STAF40, Gene Proteins, PAF350|400, Hypovolemic, CG2905, CG10549, PAF400, Temperatures, Tra1, l(2)C123, AI481500, Hot, Protein, dmTRRAP, Gene Products, PAF350/400, Heat, Hot Temperatures, Tra-1, dTRRAP, GroupVCG33554, DYKDDDDK peptide, TRRAP, l(1)VE614, MeCN, NCMe, determination, Extract, Circulatory, instrument configuration, DmelCG33554, Nip-A, protein, Spectrum Analyses, Nipped A, protein-containing complex, SDS polyacrylamide gel electrophoresis of proteins, TR-AP, Failure, CG7826, Circulatory Collapse, STAF40, peptide, Trifluoroacetate, PAF350|400, Cut, Polypeptides, 4.3.2.5, protein polypeptide chains, CG10549, peptido, Peptidylamidoglycolate lyase, Tra1, polypeptide chain, Trypsinogen (human pancreas isoenzyme 2 activation peptide), l(1)7Ba, l(1)7Bb, Hot, CH3-C#N, Mass, CG7835, CG42273, Extracts, DGS, Heat, PAL2, Min, PGL, Analysis, protein aggregate, dPAL2., Mass Spectroscopy, DmelCG42273, Mass Spectrum Analysis, Shock, Cesium Trifluoroacetate, HGAD, anon-WO0149856.5, CG5472, SDS-PAGE electrophoresis, peptides, Analyses, gel, HeLa, Nipped[A], beta-Trypsin, min, PHM, mAPC, proteins, kf, CG11387, Solution, Hypovolemic Shock, AI047805, 1.14.17.3, Hypovolemic, DmelCG11387, AI481500, DmelCG1106, sample, trifluoro-, dmTRRAP, PAF350/400, CT, peptidos, GroupV, PAL, Pal, PAM, DYRK1, peptidylamidoglycolate peptidylamide-lyase activity, 1-(14)C-labeled, 3H-labeled, study description, protein complex, Ct, Dmel_CG7826, Circulatory Failure, acetonitrile, Spectrum Analysis, 41Ah, Cell, Peptide, Spectroscopy, polypeptide, CG2905, PAF400, HeLa Cell, Temperatures, native protein, Dm1, l(2)C123, natural protein, 1-L-aspartic acid-2-L-tyrosine-3-L-lysine-, chemical analysis, Protein, peptidylamidoglycolate peptidylamide-lyase (glyoxylate-forming), Tra-1, Dyrk1, dTRRAP, HTC, BcDNA:GH10590, ACETONITRILE, Mass Spectrum Analyses, Dmel_CG7835, Acetic acid, cyanomethane, Mnb, MNB, l(2)EMS34-12, AU020952, Mass Spectrum, Temperature, Acid, NipA, instrument, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, CC1, Trifluoroacetic, Spectrometry, Tripcellim, l(2)41Ah, SDS polyacrylamide gel electrophoresis, FIGLER9, ethanenitrile, project description, tra1, alpha-hydroxyglycine amidating dealkylase activity, AW124434, dTra1, beta Trypsin, Peptidylglycine alpha-hydroxylating monooxygenase, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase, sample population, DmelCG5472, CG1106, ME-IV, Collapse, incised, Trypure, Cell Extract, Cesium, LRRC21, Cells, Hot Temperatures, Peptid, peptidyl-alpha-hydroxyglycine alpha-amidating lyase activity, assay, Polypeptide, methyl cyanide, Lrrc21falseTRRAP-interacting proteins during heat shockProject description: HeLa cells and cells stably expressing hTRRAP-3xFlAG were subjected to mass spectrometry (MS) analysis. Briefly, Cells were treated or untreated with heat shock at 42°C for 30 min, and TRRAP was immunoprecipitated from these cell extracts with 60 ul anti-FLAG M2 affinity gels by rotating at 4°C for 3 h. Immunoprecipitated sample was eluted by 40 ul FLAG peptide at 4°C for 30 min. Protein samples were loaded on 12% SDS-PAGE for preparation of gel slices. Gel bands were cut-out and subjected to in-gel digestion with trypsin. Resulting peptides were dissolved in a solution containing 0.1% trifluoroacetic acid and 2% acetonitrile and analyzed by an LTQ Orbitrap Velos Pro mass spectrometer coupled with a nanoLC instrument and HTC-PAL autosampler.Sun Jun 19 00:00:00 BST 2022PXD0318229606