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Sobota"],"species":["Homo Sapiens (human)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST001385"],"submitter_affiliation":["IMCB A-STAR Singapore"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"name_synonyms":["skin benign neoplasm, dermis plus epidermis plus hypodermis, the integument, region of skin, Oct-11a, skin, pelt, Otf-11, Skn-li, vertebrate integument, dermoid system, entire skin, skin zone, integumental organ, Skn-1a., SKIN, mKIAA0493, Epoc-1, epidermis, skin organ, portion of skin, skin plus hypodermis, vertebrate epidermis, Oct11, skin region, integument, BC033609, Fam91a1, Otf11, Skin-1a, entire integument, tegument, AV220772, Skin, dermal system, skin and subcutaneous tissue, integumentum commune"],"description_synonyms":["CDA, Forms, type 2, EP0647, PhrB photolyase activity, ban, Allergic Contact Dermatitides, bat, Progress Reports, CG17228, Strepavidin, 1135/09, combined granular-lattice corneal dystrophies, 1135/07, Polypeptides, corneal dystrophy, Biotin, Method, corneal dystrophy Avellino type, Summary Report, 0451/09, 3, NUP96, 2-amino-3-mercaptopropanoic acid, epithelium, WMS, integumentum commune, Eczematous Dermatitis, Animalia, strong, C, 0244/09, molecules, Progress Report, T-Cells, Allergic, Abp, ABP, DROPROSA, cis-Hexahydro-2-oxo-1H-thieno(3, entire skin, TINT1, T, Molekuel, procedures, Allergic Contact, SUPPRESSOR OF AUXIN RESISTANCE 3, allergic reaction, 671/2, ACD, Field Reports, scientific observation, Fam91a1, Otf11, endoplasmic reticulum auxin binding protein 1, Half Cystine, AV220772, Thymus Dependent Lymphocytes, skin and subcutaneous tissue, SGS, anatomical protrusion, Rombellin, Zinc Cysteinate, animalia, Procedure, not genetically inherited, (3aS-(3aalpha, ACMICD, DmIKKgamma, BANYULS, soluble, dIKK, Ba(0), 1316/02, dipyrimidine photolyase (photosensitive), BcDNA:HL08040, (2R)-2-amino-3-sulfanylpropanoic acid, CG5738, 1H-Thieno(3, whole organism, IKK-gamma, Field, 1167/13, AT-ERABP1, Adverse Outcome Pathway, Methodological, AVELLINO type, T-lymphocyte, allergic contact dermatitis, Report, T cell, Cells, Koerper, T Cell, l(1)16Fg, School-Age Population, entire integument, 0989/01, D-(+)-biotin, Biokur, pds, region of skin, multi-cellular organism, T4I9.14, Deacura, Otf-11, Dermatitis, number, L-Zystein, Medebiotin, Organelle., protein-containing complex, 0763/13, dIKK-gamma, anon-WO0172774.186, DmelCG17228, sensitive, DmIKK-gamma, tough, Gene Products, Allergic Eczematous, Allergic Reactions, T4I9_14, Prosp, Half-Cystine, allergic form of contact dermatitis, Gelfert, Technique, Skn-1a, LOLAL, Eczematous Dermatitides, School-Age Populations, F15E12_6, Biotin Hermes, skin, L Cysteine, E-920, F23A5.3, Hcys, Population, SKIN, Sal-1, l(3)rH013, Study, skin organ, 4-d]imidazole-4-valeric acid, Prodos, CYSTEINE, mature T cell, FREE CYSTEINE, peptidos, Lolal, (2R)-2-amino-3-mercaptopropanoic acid, Investigative Reports, TPP1, granular corneal dystrophy, 0671/02, l(3)j12C8, measuring, Oct-11a, body, Proteins, skin zone, Roche, whole body, Cell, Contact-Sensitizing, IKKgamma, skin plus hypodermis, l(3)rL433, l(2)k02512, Contact Dermatitis, l(3)rI160, native protein, 0441/16, Research Reports, chemical analysis, MFS1, KAO, Skin, ATCMPG1, MET, ATCMPG2, PIP1, skin benign neoplasm, hypersensitivity, RPE, allergic hypersensitivity disease, Gabunat, underdeveloped, Dmikkgamma, Magnetic, (R)-2-amino-3-mercaptopropanoic acid, Allergic Eczematous Dermatitides, 4)imidazole-4-valeric acid, CG16910, School Age Populations, ABP1, Abp1, 2-Amino-3-mercaptopropionic acid, Epoc-1, p. pigmentosa retinae, hypersensitivity response, l(3)rK204, plan specification, Gene Proteins, Contact Dermatitides, Reports, Abpa, E920, deoxyribonucleate pyrimidine dimer lyase (photosensitive), General activity, Allergen, l(3)rK137, the integument, E 920, DmelCG5738, pelt, Activity, vitamin B7, determination, Skn-li, T-Lymphocyte, protein, neutral molecular compounds, pigmented epithelium, School-Age, Techniques, Tier, peptido, T Lymphocyte, Hapten, 6aR)-Hexahydro-2-oxo-1H-thieno[3, Cystein, protein aggregate, animal, immature T cell, molecule, Summary Reports, IKKg, KEY, Key, Hypersensitivities, molecula, peptides, MUB3_18, cisteina, (3aS, hypoplasia, dermoid system, pigmented retina, biotina, Contact-Sensitizing Agents, biotine, MUB3.18, l(3)j6E2, DNA cyclobutane dipyrimidine photolyase activity, Progress, T Cells, 0320/10, DMPROSPER, Hermes, Methodological Studies, Reaction, biotinum, GPHYSD2, PRE, Medobiotin, l(3)rO534, PRO, Pro, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, l(2)kO2512, integumental organ, CYS, Cys, 4S, 4-d)imidazole-4-pentanoic acid, anon-EST:fe3D3, organism, PROS-1, PROS-2, Investigative Report, retinal pigment, T-Cell, School Age, pro, BC033609, Kenny, Biotin Ratiopharm, 4-d)imidazoline-4-valeric acid, and GLY protein 2, retinal pigment layer, D-Biotin, T13M11_8, PTOP, Populations, T-cell, and GLY protein 1, Biotine Roche, Voila, Thymus-Dependent, 0664/07, common, L-Cysteine, MASS, Methodological Study, TAF[[II]], Thymus-Dependent Lymphocyte, mKIAA0493, L-Cystein, DAO1, Dermatitides, phr A photolyase activity, vertebrate epidermis, DmelCG16910, DNA-photoreactivating enzyme, Agents, spine, cis-(+)-Tetrahydro-2-oxothieno[3, Polypeptide, dermal system, dermis plus epidermis plus hypodermis, 4-d]imidazol-4-yl)pentanoic acid, Allergic Eczematous Dermatitis, Thymus-Dependent Lymphocytes, Cysteine Hydrochloride, Procedures, Biotine, FBN, Gene, Vitamin H, photoreactivating enzyme activity, epidermis, protrusion, l(3)10419, dmTAF8, method, Investigative, reduced, resilient, ECTOL1, method used in an experiment, 4beta, HL-VIII, Studies, anon-WO0140519.15, Abpa27, F15E12.6, stratum pigmentosa retinae, tiny, dmIKKgamma, Animal, IKK[[gamma]], 6aalpha))-, sensitivity, PROS, MOS3, 0585/13, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, PRECOCIOUS, Coenzyme R, T13M11.8, weak, CG7128, Allergic Contact Dermatitis, metazoa, granular-lattice (Avellino) corneal dystrophy, EP647, OCTD, prod, granular and lattice corneal dystrophies, IKK, (+)-cis-Hexahydro-2-oxo-1H-thieno[3, DAO, School Age Population, TAF, 0563/18, T Lymphocytes, F23A5_3, Lymphocyte, small, Allergy, Contact Sensitizing Agents, protein complex, Biotin Gelfert, 5-(2-oxohexahydro-1H-thieno[3, Peptide, 4]imidazole-4-valeric acid, l(3)rJ806, Pros, MODIFIER OF SNC1, cis-Tetrahydro-2-oxothieno(3, Oct11, skin region, 4]imidazoline-4-valeric acid, biotin, hypersensitivity reaction type I disease, Allergies, Protein, Metazoa, deoxyribonucleic photolyase activity, TFIID, 25/12, Skin-1a, techniques, tegument, T lymphocyte, Zystein, WMS2, Biodermatin, Tcp, Biotin-Ratiopharm, Allergic Reaction, Reactions, photolyase activity, 2-amino-3-sulfanylpropanoic acid, vertebrate integument, hexahydro-2-oxo-, L-2-Amino-3-mercaptopropionic acid, Lymphocytes, Protein Gene Products, portion of skin, DmelCG7128, integument, Ban, SSKS, cardinality, Contact, Peptid, assay, TAF8, Summary, Field Report, methodology"],"additional_accession":[]},"is_claimable":false,"name":"BiotinSwitch Profiling of skin sensitizers","description":"Allergic contact dermatitis (ACD) is one of the most common form of allergies. It affects about 20% of the population.  It is a T-cell mediated hypersensitivity against contact allergens, haptens. The haptens are small electrophilic molecules that can covalently bind to nucleophilic peptides of the skin proteins. This process is called haptenation and it is the first key molecular event in the adverse outcome pathway. Due to the ban on animal testing in EU, alternative testing methods are needed to accurately access the sensitisation potential of compounds. Currently there are no efficient strategies to discover the target proteins or the pathways involved in haptenation. We report activity-based profiling (ABP) screening to identify the proteins involved in haptenation during ACD. Our approach is applicable to both haptens and pre-/pro-haptens compounds. Using this method, we have identified proteins that are potentially targeted by 17 electrophilic compounds with three different sensitizing potentials: strong, moderate and weak/non-sensitizer. The compound treated cells are harvested, lysed and proteins extracted. The soluble fraction is biotinylated with cysteine reactive biotin probe. The biotinylated proteins are enriched using streptavidin coated magnetic beads. The proteins are digested, desalted and subjected to high pH fractionation into 4 fractions. The fractionated peptides are injected into Thermo Fischer Scientific Orbitrap HF-X mass analyser coupled with EasynLC 1200. The mass spectra is acquired in data dependent acquisition mode. The acquired spectra are searched in Proteome Discoverer. The sensitizing potential of a compound can be evaluated by the number of protein targets that are identified using biotin switch assay. The common proteins that are repeatedly targeted by different sensitizers are identified and the corresponding enriched pathways and organelles were identified. ","dates":{"publication":"Thu Apr 20 00:00:00 BST 2023"},"accession":"PXD033313","cross_references":{"TAXONOMY":["9606"]}}