{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Wiff":["https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs2_SWATH.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs1_SWATH.wiff.scan","https://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont3_IDA.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont3_SWATH.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs2_IDA.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs3_IDA.wiff.scan","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs2_IDA.wiff.scan","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs1_SWATH.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs1_IDA.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont1_IDA.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs3_SWATH.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs1_IDA.wiff.scan","https://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont2_SWATH.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs3_SWATH.wiff.scan","https://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont1_SWATH.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont3_SWATH.wiff.scan","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs2_SWATH.wiff.scan","https://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont3_IDA.wiff.scan","https://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont2_IDA.wiff","https://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs3_IDA.wiff"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Krisana Asano"],"species":["Staphylococcus Aureus"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST001830"],"submitter_affiliation":["Hirosaki University"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"pubmed_abstract":["<i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> are well-known opportunistic pathogens that frequently coexist in chronic wounds and cystic fibrosis. The exoproducts of <i>P. aeruginosa</i> have been shown to affect the growth and pathogenicity of <i>S. aureus,</i> but the detailed mechanisms are not well understood. In this study, we investigated the effect of extracellular vesicles from <i>P. aeruginosa</i> (PaEVs) on the growth of <i>S. aureus</i>. We found that PaEVs inhibited the <i>S. aureus</i> growth independently of iron chelation and showed no bactericidal activity. This growth inhibitory effect was also observed with methicillin-resistant <i>S. aureus</i> but not with <i>Acinetobacter baumannii, Enterococcus faecalis, S.</i> Typhimurium, <i>E. coli, Listeria monocytogenes</i>, or <i>Candida albicans</i>, suggesting that the growth inhibitory effect of PaEVs is highly specific for <i>S. aureus</i>. To better understand the detailed mechanism, the difference in protein production of <i>S. aureus</i> between PaEV-treated and non-treated groups was further analyzed. The results revealed that lactate dehydrogenase 2 and formate acetyltransferase enzymes in the pyruvate fermentation pathway were significantly reduced after PaEV treatment. Likewise, the expression of <i>ldh2</i> gene for lactate dehydrogenase 2 and <i>pflB</i> gene for formate acetyltransferase in <i>S. aureus</i> was reduced by PaEV treatment. In addition, this inhibitory effect of PaEVs was abolished by supplementation with pyruvate or oxygen. These results suggest that PaEVs inhibit the growth of <i>S. aureus</i> by suppressing the pyruvate fermentation pathway. This study reported a mechanism of PaEVs in inhibiting <i>S. aureus</i> growth which may be important for better management of <i>S. aureus</i> and <i>P. aeruginosa</i> co-infections."],"pubmed_title":["Extracellular vesicles of <i>Pseudomonas aeruginosa</i> downregulate pyruvate fermentation enzymes and inhibit the initial growth of <i>Staphylococcus aureus</i>."],"pubmed_authors":["Ishiai Takahito T, Subsomwong Phawinee P, Narita Kouj K, Kawai Noriaki N, Teng Wei W, Suzuki Sachio S, Sukchawalit Rojana R, Nakane Akio A, Asano Krisana K"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic analysis of Staphylococcus aureus after treating with extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)","description":"For proteomic analysis of PaOMV-treated S. aureus, S. aureus (5 × 103 CFU/mL) was incubated with and without 1 µg/mL PaOMVs for 6 h at 37℃ under static condition and proteomic analysis was performed as described above. The identified proteins were searched against S. aureus ATCC1718 (NCBI Genebank: NC_007795.1). In comparison with non-PaOMV treatment, differential protein expression in PaOMV-treated S. aureus was assessed by quantitative proteomics using SWATH analysis.","dates":{"publication":"Mon May 08 00:00:00 GMT+01:00 2023"},"accession":"PXD036381","cross_references":{"TAXONOMY":["1280"],"pubmed":["37131486"]}}