JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs2_SWATH.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs1_SWATH.wiff.scanhttps://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont3_IDA.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont3_SWATH.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs2_IDA.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs3_IDA.wiff.scanhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs2_IDA.wiff.scanhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs1_SWATH.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs1_IDA.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont1_IDA.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs3_SWATH.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs1_IDA.wiff.scanhttps://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont2_SWATH.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs3_SWATH.wiff.scanhttps://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont1_SWATH.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont3_SWATH.wiff.scanhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs2_SWATH.wiff.scanhttps://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont3_IDA.wiff.scanhttps://storage.jpostdb.org/JPST001830/files/210623_Sa_Cont2_IDA.wiffhttps://storage.jpostdb.org/JPST001830/files/210623_Sa+PaOMVs3_IDA.wiffprimary200OKProteomicsKrisana AsanoStaphylococcus Aureushttps://repository.jpostdb.org/entry/JPST001830Hirosaki UniversityjPOST<i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> are well-known opportunistic pathogens that frequently coexist in chronic wounds and cystic fibrosis. The exoproducts of <i>P. aeruginosa</i> have been shown to affect the growth and pathogenicity of <i>S. aureus,</i> but the detailed mechanisms are not well understood. In this study, we investigated the effect of extracellular vesicles from <i>P. aeruginosa</i> (PaEVs) on the growth of <i>S. aureus</i>. We found that PaEVs inhibited the <i>S. aureus</i> growth independently of iron chelation and showed no bactericidal activity. This growth inhibitory effect was also observed with methicillin-resistant <i>S. aureus</i> but not with <i>Acinetobacter baumannii, Enterococcus faecalis, S.</i> Typhimurium, <i>E. coli, Listeria monocytogenes</i>, or <i>Candida albicans</i>, suggesting that the growth inhibitory effect of PaEVs is highly specific for <i>S. aureus</i>. To better understand the detailed mechanism, the difference in protein production of <i>S. aureus</i> between PaEV-treated and non-treated groups was further analyzed. The results revealed that lactate dehydrogenase 2 and formate acetyltransferase enzymes in the pyruvate fermentation pathway were significantly reduced after PaEV treatment. Likewise, the expression of <i>ldh2</i> gene for lactate dehydrogenase 2 and <i>pflB</i> gene for formate acetyltransferase in <i>S. aureus</i> was reduced by PaEV treatment. In addition, this inhibitory effect of PaEVs was abolished by supplementation with pyruvate or oxygen. These results suggest that PaEVs inhibit the growth of <i>S. aureus</i> by suppressing the pyruvate fermentation pathway. This study reported a mechanism of PaEVs in inhibiting <i>S. aureus</i> growth which may be important for better management of <i>S. aureus</i> and <i>P. aeruginosa</i> co-infections.Extracellular vesicles of <i>Pseudomonas aeruginosa</i> downregulate pyruvate fermentation enzymes and inhibit the initial growth of <i>Staphylococcus aureus</i>.Ishiai Takahito T, Subsomwong Phawinee P, Narita Kouj K, Kawai Noriaki N, Teng Wei W, Suzuki Sachio S, Sukchawalit Rojana R, Nakane Akio A, Asano Krisana KfalseProteomic analysis of Staphylococcus aureus after treating with extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)For proteomic analysis of PaOMV-treated S. aureus, S. aureus (5 × 103 CFU/mL) was incubated with and without 1 µg/mL PaOMVs for 6 h at 37℃ under static condition and proteomic analysis was performed as described above. The identified proteins were searched against S. aureus ATCC1718 (NCBI Genebank: NC_007795.1). In comparison with non-PaOMV treatment, differential protein expression in PaOMV-treated S. aureus was assessed by quantitative proteomics using SWATH analysis.Mon May 08 00:00:00 GMT+01:00 2023PXD036381128037131486