{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xlsx":["https://storage.jpostdb.org/JPST002032/files/Result_KKU055_PTS_global_protein%20and%20peptide.xlsx"],"Raw":["https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_SP_72h_PTS_2.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_Dif_72h_PTS_4.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_P_72h_PTS_1.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_Dif_72h_PTS_2.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_P_72h_PTS_3.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_SP_72h_PTS_1.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_SP_72h_PTS_3.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_Dif_72h_PTS_3.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_P_72h_PTS_2.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_P_72h_PTS_4.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_SP_72h_PTS_4.raw","https://storage.jpostdb.org/JPST002032/files/20210206_Easy_0118Nikkyo_240min_2ug_KKU055_Dif_72h_PTS_1.raw"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Norie Araki"],"species":["Homo Sapiens (human)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST002032"],"submitter_affiliation":["Kumamoto University"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"pubmed_abstract":["Cholangiocarcinoma (CCA) is an aggressive malignant tumor of bile duct epithelia. Recent evidence suggests the impact of cancer stem cells (CSC) on the therapeutic resistance of CCA; however, the knowledge of CSC in CCA is limited due to the lack of a CSC model. In this study, we successfully established a stable sphere-forming CCA stem-like cell, KKU-055-CSC, from the original CCA cell line, KKU-055. The KKU-055-CSC exhibits CSC characteristics, including: (1) the ability to grow stably and withstand continuous passage for a long period of culture in the stem cell medium, (2) high expression of stem cell markers, (3) low responsiveness to standard chemotherapy drugs, (4) multilineage differentiation, and (5) faster and constant expansive tumor formation in xenograft mouse models. To identify the CCA-CSC-associated pathway, we have undertaken a global proteomics and functional cluster/network analysis. Proteomics identified the 5925 proteins in total, and the significantly upregulated proteins in CSC compared with FCS-induced differentiated CSC and its parental cells were extracted. Network analysis revealed that high mobility group A1 (HMGA1) and Aurora A signaling through the signal transducer and activator of transcription 3 pathways were enriched in KKU-055-CSC. Knockdown of HMGA1 in KKU-055-CSC suppressed the expression of stem cell markers, induced the differentiation followed by cell proliferation, and enhanced sensitivity to chemotherapy drugs including Aurora A inhibitors. In silico analysis indicated that the expression of HMGA1 was correlated with Aurora A expressions and poor survival of CCA patients. In conclusion, we have established a unique CCA stem-like cell model and identified the HMGA1-Aurora A signaling as an important pathway for CSC-CCA."],"pubmed_title":["Establishment and characterization of a novel cancer stem-like cell of cholangiocarcinoma."],"pubmed_authors":["Panawan Orasa O, Silsirivanit Atit A, Chang Chih-Hsiang CH, Putthisen Siyaporn S, Boonnate Piyanard P, Yokota Taro T, Nishisyama-Ikeda Yuki Y, Detarya Marutpong M, Sawanyawisuth Kanlayanee K, Kaewkong Worasak W, Muisuk Kanha K, Luang Sukanya S, Vaeteewoottacharn Kulthida K, Kariya Ryusho R, Yano Hiromu H, Komohara Yoshihiro Y, Ohta Kunimasa K, Okada Seiji S, Wongkham Sopit S, Araki Norie N"],"pubmed_abstract_synonyms":["NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR D, Product, determination, Laboratory, Heterograft, Feature, Mus domesticus, Intrahepatic Cholangiocarcinomas, TRANSPL HETEROL, Tumor, Intrahepatic, type 9, House Mouse, Cell Growth in Number, dmTAF[[II]]230, Background, DSmurf, congenital, Cultural, Pharmaceutical Product, Mother Cells, Line, Cell Number Growth, Scanning Transmission Electron Microscopy, 3, HMGA1A, primary stem, contractural arachnodactyly, multicellular organismal biosynthetic process, arachnodactyly, imprinted and ancient gene protein, Hmga1a, Ear anomalies-contractures-dysplasia of bone with kyphoscoliosis, ethnicity, single-organism biosynthetic process, me75, Growth, 8-[(E)-2-(3-chlorophenyl)ethenyl]-1, TFIID TAF250, cel, long, Extrahepatic, Swiss Mice, D17Mit170, T1, Hmga1b, allergic reaction, intrahepatic bile duct cancer (cholangiocarcinoma), contractures, 6-dione, Xenotransplantations, malignant neoplasm, medicine, Smurf, Pharmaceutical, Cholangiocarcinomas, Therapies, xenotransplant, Malignancies, 7-dihydro-1H-purine-2, house mouse, associated, transcription from bacterial-type RNA polymerase promoter, 7-trimethyl-3, CG4943, single organism signaling, Tumors, D-smurf, Therapy, Carcinoma, dTAF[[II]]230, HMGIY, high mobility group, mouse, TAF200, Lack, TAFII-250, TAF250/230, Tl3, Cholangiocellular, Tl2, Electron Microscopy, Menstruation, TAFII250, Cultural Background, Benign, Colony-Forming Unit, Stem Cell, Cultures, Cholangiocellular Carcinomas, Chemotherapy, Cellular, Pharmaceutic, Heterologous Transplantations, Hmgiy, Intrahepatic., Lines, Carcinomas, Mother Cell, Xenograft, Mus musculus, Epistemology, Xenotransplantation, Pharmacotherapy, Cultural Beliefs, death rate, DmelCG4943, Transplantation, mice, Stem, Swiss Mouse, Benign Neoplasms, Colony Forming Units, Xenografts, CG17603, Features, TAF[[II]], Treatments, Malignant Neoplasms, domesticus, animal stem cell, Hmgi, primary axis, Taf250, Specificity and Sensitivity, antagonists and inhibitors, Patient, SR3-5, Proliferation, bacterial transcription, Cells, STEM, Cell Number, distal, Mouse, Hmgy, E430016J11Rik, other neoplasm, TAF230, d230, Extrahepatic Cholangiocarcinoma, Peptidomics, chemotherapy, Neoplasms, dSmurf1, Colony-Forming Units, Benign Neoplasm, Progenitor Cell, Gene, stalk, dTAFII250, pharmacotherapy, Pharmacotherapies, Transplantations, Ximpact, Malignant, EfW1, Chemotherapies, heterologous transplantation, dmTAF1, House, multiple with arachnodactyly, Taf230, sensitive, resistance, Gene Products, Mus musculus domesticus, Low, Number Growth, Mice, Progenitor, Drug Therapies, sensitivity, HMG-R, Backgrounds, TAF250, Cholangiocellular Carcinoma, Drugs, study, Taf200, network topology analysis, dTAF[[II]]250, Malignancy, cell, Swiss, Transducer, Heterografts, inhibiteur, Mother, Taf1p, Cultural Relativisms, DA9, culm, Neoplasias, dTAF250, Intrahepatic Cholangiocarcinoma, cell proliferation, drugs, d-smurf, inhibidor, time of survival, Clients, Customs, Sensitivity, ATHMG, Characteristics, culture, Preparation, TAF, Heterologous, Cancer, Pharmaceuticals, inhibitors, Products, Multiplication, Colony Forming Unit, TAF[[II]]250, Cellular Proliferation, Malignant Neoplasm, cou, bile duct cancer, axis, NFD, Progenitor Cells, arthrogryposis, Proteins, Cell Lines, l(3)84Ab, inhibitor, Scanning Transmission, BG:DS00004.13, Cultural Backgrounds, Medications, Dsmurf, Client, impact-a, Cell, dTAF230, contractural Beals type, dSmurf, Lr, MT, Characteristic, XENOTRANSPL, survival, AL023995, Period, Mus, p230, Smurf ubiquitin ligase, Protein, chemical analysis, Neoplasm, TAF[[II]]250/230, imprinted and ancient gene protein homolog, IMPACT, TFIID, Extrahepatic Cholangiocarcinomas, antagonists, Taf[[II]]250, primary cancer, TAF[[II]]230, Pharmaceutic Preparations, CSC, Specificity, stem cell, HETEROL TRANSPL, House Mice, TAF[II]250, Cancers, malignant tumor, Cholangiocarcinoma, Laboratory Mice, CCA, Drug, Protein Gene Products, EOMD, Gene Proteins, Preparations, DmelCG17603, signalling process, Therapeutic, SSRP1, Cell Multiplication, Bra, Relativisms, Treatment, pharmacologic therapy, assay, Relativism, Cultural Relativism, Pharmaceutical Products, Laboratory Mouse, RWDD5, Neoplasia, Pharmaceutical Preparation, TAF1"],"name_synonyms":["Cholangiocellular Carcinoma, Carcinomas, Mother Cell, Peptidomics., Carcinoma, Colony Forming Unit, Extrahepatic Cholangiocarcinoma, bile duct cancer, Stem, Progenitor Cells, Colony-Forming Units, Intrahepatic Cholangiocarcinomas, Extrahepatic, Progenitor Cell, Mother, Colony Forming Units, Intrahepatic, Cholangiocellular, Cell, Cholangiocarcinoma, intrahepatic bile duct cancer (cholangiocarcinoma), Intrahepatic Cholangiocarcinoma, Colony-Forming Unit, Stem Cell, Cholangiocellular Carcinomas, Cholangiocarcinomas, Cells, Mother Cells, Extrahepatic Cholangiocarcinomas, Progenitor"],"description_synonyms":["Mother Cell, Cholangiocellular Carcinoma, Carcinomas, Carcinoma, Colony Forming Unit, Extrahepatic Cholangiocarcinoma, bile duct cancer, Stem, Progenitor Cells, Colony-Forming Units, Intrahepatic Cholangiocarcinomas, Progenitor Cell, Extrahepatic, total expressed protein, Mother, Colony Forming Units, Intrahepatic, Cholangiocellular, Cell, Cholangiocarcinoma, intrahepatic bile duct cancer (cholangiocarcinoma), Intrahepatic Cholangiocarcinoma, Colony-Forming Unit, Stem Cell, Cholangiocellular Carcinomas, Cells, Cholangiocarcinomas, Mother Cells, Extrahepatic Cholangiocarcinomas, Proteomes, Progenitor, Intrahepatic."],"pubmed_title_synonyms":["Cholangiocellular Carcinoma, Carcinomas, intrahepatic bile duct cancer (cholangiocarcinoma), Intrahepatic Cholangiocarcinoma, Carcinoma, Extrahepatic Cholangiocarcinoma, bile duct cancer, Cholangiocellular Carcinomas, Cholangiocarcinomas, Intrahepatic Cholangiocarcinomas, Extrahepatic, Krebsstammzelle, CSC cell, cancer stem-like cell, Extrahepatic Cholangiocarcinomas, Intrahepatic, Cholangiocellular, Intrahepatic., Cholangiocarcinoma"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of the cholangiocarcinoma stem cells by proteomics","description":"Comparison of global proteome between stem cells, differentiating stem cells, and parental cells of cholangiocarcinoma.","dates":{"publication":"Thu Feb 08 00:00:00 GMT 2024"},"accession":"PXD039953","cross_references":{"TAXONOMY":["9606"],"pubmed":["37026527"]}}