JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST002154/files/Table%20S1.xlsxhttps://storage.jpostdb.org/JPST002154/files/C_3.mzXMLhttps://storage.jpostdb.org/JPST002154/files/D_2.mzXMLhttps://storage.jpostdb.org/JPST002154/files/C_2.mzXMLhttps://storage.jpostdb.org/JPST002154/files/B_3.mzXMLhttps://storage.jpostdb.org/JPST002154/files/B_2.mzXMLhttps://storage.jpostdb.org/JPST002154/files/A_3.mzXMLhttps://storage.jpostdb.org/JPST002154/files/D_1.mzXMLhttps://storage.jpostdb.org/JPST002154/files/C_1.mzXMLhttps://storage.jpostdb.org/JPST002154/files/A_2.mzXMLhttps://storage.jpostdb.org/JPST002154/files/B_1.mzXMLhttps://storage.jpostdb.org/JPST002154/files/A_1.mzXMLhttps://storage.jpostdb.org/JPST002154/files/D_3.mzXMLhttps://storage.jpostdb.org/JPST002154/files/D_1.mgfhttps://storage.jpostdb.org/JPST002154/files/B_2.mgfhttps://storage.jpostdb.org/JPST002154/files/C_3.mgfhttps://storage.jpostdb.org/JPST002154/files/C_1.mgfhttps://storage.jpostdb.org/JPST002154/files/B_3.mgfhttps://storage.jpostdb.org/JPST002154/files/C_2.mgfhttps://storage.jpostdb.org/JPST002154/files/A_3.mgfprimaryOK200ProteomicsDr. Amit PrasadTaenia Soliumhttps://repository.jpostdb.org/entry/JPST002154Indian Institute of Technology MandijPOSTThe excretory-secretory proteome plays a pivotal role in both intercellular communication during disease progression and immune escape mechanisms of various pathogens including cestode parasites like Taenia solium. The cysticerci of T. solium causes infection in the central nervous system known as neurocysticercosis (NCC), which affects a significant population in developing countries. Extracellular vesicles (EVs) are 30-150-nm-sized particles and constitute a significant part of the secretome. However, the role of EV in NCC pathogenesis remains undetermined. Here, for the first time, we report that EV from T. solium larvae is abundant in metabolites that can negatively regulate PI3K/AKT pathway, efficiently internalized by macrophages to induce AKT and mTOR degradation through auto-lysosomal route with a prominent increase in the ubiquitination of both proteins. This results in less ROS production and diminished bacterial killing capability among EV-treated macrophages. Due to this, both macro-autophagy and caspase-linked apoptosis are upregulated, with a reduction of the autophagy substrate sequestome 1. In summary, we report that T. solium EV from viable cysts attenuates the AKT-mTOR pathway thereby promoting apoptosis in macrophages, and this may exert immunosuppression during an early viable stage of the parasite in NCC, which is primarily asymptomatic. Further investigation on EV-mediated immune suppression revealed that the EV can protect the mice from DSS-induced colitis and improve colon architecture. These findings shed light on the previously unknown role of T. solium EV and the therapeutic role of their immune suppression potential.Taenia solium cysticerci's extracellular vesicles Attenuate the AKT/mTORC1 pathway for Alleviating DSS-induced colitis in a murine model.Rawat Suraj Singh SS, Keshri Anand Kumar AK, Arora Naina N, Kaur Rimanpreet R, Mishra Amit A, Kumar Rajiv R, Prasad Amit AfalseInvestigating the proteomic content of extracellular vesicles from Taenia solium cysticerciWe have employed the stack gel method to analyze the proteomic content of EVs. Briefly, 50 ug of EVs sample was lysed using RIPA buffer with 1 mM PMSF for 10 minutes in ice with occasional vortexing. peptide solution was transferred to the tube containing 50 mM ammonium bicarbonate and the step with 50% v/v acetonitrile, 5% formic acid was repeated again. Following this, the peptide solution was dried using a vacuum centrifuge. The peptide pellet was then resuspended in peptide loading buffer (0.1% v/v formic acid, pH 3.5) which was then subjected to peptide identification using a 6560 Ion Mobility LC/Q-TOF (Bruker Daltonics Inc.). Germany). Peptide fingerprint was analyzed using Spectrum Mill software (Release year 2017).Wed Aug 26 00:00:00 BST 2026PXD042112620438779712