{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Raw":["https://storage.jpostdb.org/JPST002156/files/AS20200226S1-BS3.raw","https://storage.jpostdb.org/JPST002156/files/AS20200123S1-DSG.raw","https://storage.jpostdb.org/JPST002156/files/DID-DAD-BS3-S2.raw","https://storage.jpostdb.org/JPST002156/files/AS20200226S2-BS(PEG)5.raw"],"Fasta":["https://storage.jpostdb.org/JPST002156/files/DID_DAD.Fasta"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Alexander Shekhtman"],"species":["Homo Sapiens (human)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST002156"],"submitter_affiliation":["SUNY Albany"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"name_synonyms":["DFNA1, D18Wsu154e, Diaph1, hDIA1, Activity, p140mDia, General activity., DIA1, Dia1, DRF1, Drf1, mKIAA4062, LFHL1, results"],"description_synonyms":["sodium salt, Diaphanous-Related Formin Homology Proteins, ammonium formate, 13C-labeled, cadmium salt, MeCN, nickel formate dihydrate, NCMe, lead formate, determination, region or site annotation, Diaphanous-Related Formins, aluminum salt, cesium salt, zinc formate, Gene, lead salt, DIAPH Protein 1, Gel Electrophoresis, DIAPH Protein 2, magnesium formate, Diaphanous Related Formin Homology Proteins, SDS polyacrylamide gel electrophoresis of proteins, zinc salt, formic acid, FMN Proteins, Polypeptides, Sodium Dodecyl Sulfate PAGE, potassium salt, ammonium tetraformate, peptido, cobalt(II) formate dihydrate, 14C-labeled, Formin 2 Protein, CH3-C#N, Polyacrylamide Gel Electrophoresis, Gene Products, SDS-PAGE, Formin Homology Proteins, positional, Polyacrylamide, SDS-PAGE electrophoresis, peptides, thallium (+1) salt, MS2, M, beta-Trypsin, rubidium salt, DIAPH Proteins, chemical analysis., copper, calcium formate, methanoic acid, Formins, Diaphanous-Related, geographical area, lithium salt, FMN Protein 2, peptidos, cromium (+3) salt, FMN Protein 1, ammonium (2:1) salt, sodium formate, SDS PAGE, nickel salt, nickel formate, 1-(14)C-labeled, 3H-labeled, Molars, cobalt (+2) salt, molar, Proteins, strontium formate, potassium formate, copper (+2) salt, strontium salt, acetonitrile, motif, results, Peptide, sodium (4:1:1) salt, dens molaris, magnesium salt, cupric formate, Protein, sequence, aluminum formate, nickel (+2) salt, ACETONITRILE, tandem MS, cyanomethane, ammonium salt, lithium formate, PRSS, positional polypeptide feature, Sodium Dodecyl Sulfate-PAGE, MS/MS, Formin, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, formate, Tripcellim, cobaltous formate, SDS polyacrylamide gel electrophoresis, cromium (+3), ethanenitrile, beta Trypsin, primary structure of sequence macromolecule, chromic formate, Protein Gene Products, Gene Proteins, Trypure, concentration, copper salt, ammonium (4:1) salt, calcium salt, Diaphanous Related Formins, Formin 1 Protein, Peptid, Polypeptide, assay, methyl cyanide, Sodium Dodecyl Sulfate-PAGEs, lead (+2) salt"],"additional_accession":[]},"is_claimable":false,"name":"Disruption of the Productive Encounter Complex Results in Dysregulation of DIAPH1 Activity","description":"Formins are characterized by a N-terminal regulatory domain, which binds to the C-terminal auto-inhibitory domain, yielding an inactive complex.\n\nCross-Linking and tandem MS was used to characterized the disordered \"RRKR\" motif of the C-terminus, which is notably absent from solved crystallographic structures. These results were used with NMR spectroscopy to locate and understand the dynamics of this disordered region.\n\nThe proteins were synthesized using recombinant techniques and purified using chromatographic techniques. Proteins were mixed in equi-molar concentrations with excess concentration of cross-linkers. Samples were analyzed using SDS-PAGE gels, excised for chemical processing, and subjected to Trypsin digestion. Resulting peptides were extracted using formic acid and acetonitrile, lyophilized, and stored until MS analysis","dates":{"publication":"Thu May 11 00:00:00 BST 2023"},"accession":"PXD042130","cross_references":{"TAXONOMY":["9606"]}}