JPOST 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FukataMus Musculus (mouse)https://repository.jpostdb.org/entry/JPST002419Nagoya University Graduate School of MedicinejPOSTthe brain, MGC130048, FLN-B, SCT, SGCG_HUMAN, Laboratory, Ly-6A.2/Ly-6E.1, Peptidomics, Protein USO1 homolog, Mus domesticus, Abcb2, A4, Ly-6A|E, Stem cell antigen 1, House Mouse, 1300013M05Rik, TYPE, Ham1, DAGA4, Ly6, TAP1, House, hTAP, Mex67h, 35DAG, Mus musculus domesticus, AW536553, synganglion, MAM, gamma-SG, SCG3, Mice, Ham-1, gamma sarcoglycan, ABP-280-like protein, Ly-6A/E, Sca1, Swiss, Swiss Mice, suprasegmental levels of nervous system, ABC17, MCP-IV, TABP, Vdp, VDP, LRS1, sample, CG7659, gamma-sarcoglycan, stem cell antigen 1, house mouse, Ly-6A.2|Ly-6E.1, 115kDa, Squalene transfer protein, Ly6a, FLN1L, C22orf6, TAP, Tap, ABP-278, suprasegmental structures, Spf, SPF, Ly-6A.2, 35 kDa dystrophin-associated glycoprotein, bHLHc9, SG-gamma, mouse, Ly-6E.1, Sca-1, encephalon, SGCG, LGMD2C, RING4, tap, Mus, Tap-1, FH1, sarcoglycan, Mvb1, ABP-280, Mus musculus, DmelCG7659, DMDA1, Alpha-tocopherol-associated protein, FLN3, PSF1, mice, AI256582, Encephalon, Swiss Mouse, APT1, LFQ., House Mice, gamma (35kDa dystrophin-associated glycoprotein), AOI, Supernatant protein factor, MEX67, sample population, domesticus, Laboratory Mice, P115, bps, SCA-1, DMDA, Transcytosis-associated protein, 35kD dystrophin-associated glycoprotein, MTP1, Mex67, SCARMD2, Mouse, Vesicle-docking protein, Y3, Laboratory Mouse, l(3)01658, T-cell-activating protein, dipeptide chemoreceptor proteinl(2)k04204, MGC130048, FLN-B, SCT, Affinity Purification, ion, Tandem, determination, Laboratory, Mus domesticus, Abcb2, classic hairy cell leukaemia, Ly-6A|E, A4, Monohydrate, Guanidine Sulfate (2:1), LFQ, Hydrogenchlorid, 6330543G17Rik, protein, House Mouse, prevention, trichloro-, Ham1, 2-Propanone, peptide, Polypeptides, hydrogen chloride, Ly6, peptido, hTAP, B1, 1, 2, Software Engineering, protein aggregate, prevention and control, methyl ketone, Computer Program, (R*, gamma sarcoglycan, Sca1, reference sample, peptides, Rubidium, Open, hypoplasia, Su(Mir)1, Computer Programs and Programming, Swiss Mice, Phosphate, ABC17, Trichloroacetic, preventive measures, Tandem Affinity, Wasserstoffchlorid, LRS1, column, Propanon, Sputolysin, D1, Chromatography, CG7659, gamma-sarcoglycan, Sodium Trichloroacetate, l(2)CA4, stem cell antigen 1, Guanidine Sulfate (1:1), house mouse, 115kDa, Prx3, preventive therapy, Monohydrochloride, Ly-6A.2, Tandem Affinity Chromatographies, bHLHc9, dimethylketone, SG-gamma, mouse, Liquid Chromatography, Guanidine Monohydroiodine, 4-dimercapto-2, classic hairy cell leukemia, Aceton, Source Softwares, shortstop/kakapo, Software Tools, Programs, RING4, Program, Computer Applications, Pyroacetic ether, Cleland, Ionen, Tap-1, DmelCG18076, Computer Applications Software, Chlorwasserstoff, Computer Applications Softwares, sarcoglycan, Softwares, Acetic acid, Mus musculus, Acid, DmelCG7659, Software Applications, Grv, FLN3, AI256582, mice, Source Software, HCl, Swiss Mouse, APT1, Guanidine Phosphate, Guanidine Monohydrochloride, 3-Butanediol, gamma (35kDa dystrophin-associated glycoprotein), beta-Ketopropane, beta Trypsin, MEX67, SHOT, domesticus, R*)-1, Applications, DMDA, Acide, grv, 35kD dystrophin-associated glycoprotein, Acetamide, Mex67, Mouse, Polypeptide, chlorane, Y3, 4-Dimercapto-2, l(3)01658, T-cell-activating protein, dipeptide chemoreceptor protein, 42/4, Proteomes, Affinity Chromatography, Computer Software Applications, Cleland Reagent, rac-Dithiothreitol, OG12X, Acide trichloracetique, SGCG_HUMAN, Ly-6A.2/Ly-6E.1, chlorure d'hydrogene, threo-1, Tandem Affinity Chromatography, Protein USO1 homolog, Reagent, Cleland's reagent, Gene, 4-dimercapto-, Computer, Stem cell antigen 1, R*)-, protein-containing complex, 1300013M05Rik, TYPE, Buffer, DAGA4, l(2)k05821, propanone, Guanidium, TAP1, reduced, l(2)k15606, House, Mex67h, 35DAG, dimethylcetone, Gene Products, Mus musculus domesticus, AW536553, tiny, MAM, gamma-SG, trichloracetique, SCG3, Mice, cloruro de hidrogeno, Ham-1, Shot, Application, Guanidium Chloride, Open Source Softwares, ABP-280-like protein, Ly-6A/E, Monohydroiodine, l(2)k10821, Hydrogen chloride, Swiss, iones, Software Application, beta-Trypsin, Open Source Software, Sciences, MCP-IV, ions, TABP, Computer Software Application, Vdp, VDP, Monohydrobromide, Guanidinium Chloride, Tools, peptidos, Ly-6A.2|Ly-6E.1, Azeton, Dimethyl ketone, Squalene transfer protein, Guanidine Hydrochloride, Ly6a, 2-iodo-, statistical analysis, FLN1L, PTHB1, C22orf6, Controlled, small, 3-butanediol, Applications Software, TAP, Tap, Open Source, ABP-278, Controlling, Dimethylketon, Guanidine Monohydrate, Guanidine Nitrate, Spf, SPF, Computer Software, 35 kDa dystrophin-associated glycoprotein, protein complex, kop, Proteins, CG18076, Clelands Reagent, total expressed protein, Ly-6E.1, Sca-1, chloridohydrogen, CG18637, buffer, Guanidinium, Dithiothreitol, Peptide, SGCG, LGMD2C, Tool, Dithiotreitol, tap, polypeptide, [HCl], Software Tool, native protein, Mus, C18, Protein, FH1, chemical analysis, DL-threo-1, Trichloroacetate, Hydrochloride, Guanidine Sulfite (1:1), Nitrate, Sodium, Rubidium Trichloroacetate, Software, Mvb1, ABP-280, DTL, Cleland's, Guanidine Monohydrobromide, l(2)k05434, DTT, Guanidine, PRSS, Guanidine Sulfate, DMDA1, Alpha-tocopherol-associated protein, underdeveloped, PSF1, Protein., prophylaxis, l(2)k03010, Engineering, Tripcellim, Chloride, kak, House Mice, AOI, Supernatant protein factor, OG12, Laboratory Mice, Protein Gene Products, P115, Cleland's Reagent, Computer Programs, Gene Proteins, Trypure, bps, SCA-1, Transcytosis-associated protein, Applications Softwares, Ion, control, MTP1, SCARMD2, Peptid, assay, Og12x, Vesicle-docking protein, Laboratory Mouse, Sulfate, hydrochloric acidfalsewild-type and LGI3-FH KI mouse brain TAP sample, Shotgun proteomics with label-free quantificationThe proteins in the tandem affinity purification (TAP) eluates from the wild-type control and Lgi3-FH KI mouse brains were concentrated by Trichloroacetic acid/acetone precipitation and redissolved in the denaturing buffer containing 7 M guanidine hydrochloride, 0.5M Tris-HCl (pH 8.5), and 10 mM EDTA. Then, proteins were reduced with dithiothreitol and alkylated with iodoacetamide, concentrated, and digested with trypsin at 37°C overnight (in-solution digestion). The obtained peptides were separated via nano-flow liquid chromatography (EASY-nLC1000, Thermo Fisher Scientific) using a reverse-phase C18 column (0.075 Å~ 125 mm; Nikkyo Technos). The liquid chromatography eluent was coupled to a nano ion spray source attached to an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). For shot-gun analysis including protein identification, label-free quantification, and volcano plot analyses, the Mascot2.6.1 (Matrix Science) and Proteome Discoverer2.2 software (Thermo Fisher Scientific) were used. Peptide and protein identifications were calculated with protein false discovery rate (FDR) < 0.01. No filtering was applied and obtained protein lists with a high FDR confidence (< 0.01) from control and LGI3-FH KI replicates were used for statistical analysis to compare protein abundances based on peak intensity.Thu Dec 14 00:00:00 GMT 2023PXD04779010090