JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST002440/files/20230627_sample2c-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample2b-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample2a-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample3a-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample3b-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample3c-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample3b-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample1c-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample4a-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample1b-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample1a-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample2c-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample2a-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample1c-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample4a-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample3a-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample2b-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample3c-t2.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample1b-t1.rawhttps://storage.jpostdb.org/JPST002440/files/20230627_sample1a-t1.rawprimaryOK200ProteomicsItaru HamachiHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST002440Kyoto UniversityjPOSTliquid chromatography tandem mass spectroscopy, LC-MS2, cuivre, Co-Immunoprecipitations, other disease, tx, Probe, Atp7a, Kupfer, 29Cu, cobre, Proteins, LC-MS/MS, disorders, Cu, Gene, LFQ, medical condition, cuprum, Copper, Probes, Cell, Cun, MK, LC-MS-MS, Impact, Co Immunoprecipitation, Environmental, Copper 63, LC/MS/MS, diseases, Cu(0), Protein, LC-MSMS, CG9063, Gene Products, DSMAX, Immune Precipitations, disease or disorder, Impacts, condition, diseases and disorders, Environmental Impacts, Immune Precipitation, non-neoplastic., human disease, PRSS, LCMSMS, MNK, Mnk, Molecular, WND, BcDNAGH03694, Co-Immunoprecipitation, beta-Trypsin, Precipitation, Tripcellim, labeling, copper, BcDNA:GH03694, beta Trypsin, 3L6, Protein Gene Products, Gene Proteins, Environmental Impact, Trypure, disease, Immune, rich, liquid chromatography-tandem mass spectroscopy, Environments, liquid chromatography tandem mass spectrometry, disorder, Homo sapiens disease, Copper-63, Molecular Probe, SMAX3, Precipitations, DmelCG9063tx, reactivo, pre-mortem, MNK, Mnk, protein complex, WND, Reagent, Atp7a, Proteins, total expressed protein, Gene, reagent, labeling, protein, protein-containing complex, Indicators, MK, Protein Gene Products, Gene Proteins, living, native protein, Protein, Indicator, Gene Products, DSMAX, Cell., Reagents, SMAX3, protein aggregate, reactif, Proteomes, Reagents and Indicators, reagentsfalseProteome labeled by a cuprous ion-responsive protein labeling reagent in the ATP7A WT and ATP7A KO living cellsWe have developed a new molecular probe called CuR. This probe is selectively activated by labile Cu(I), hence enabling labeling of the proteins in Cu(I)-rich cellular environments. We applied CuR to ATP7A-WT and ATP7A-KO cells that were pretreated with or without Cu(gtsm). Next, we enriched the labeled proteins by immunoprecipitation, digested them with trypsin, and analyzed them using liquid chromatography-tandem mass spectrometry. We used label-free quantification in the mass spectrometry analysis to detect changes in labeled proteins caused by exogenous copper compared to the non-copper-treated condition.Sun Jan 05 00:00:00 GMT 2025PXD0482679606