{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xlsx":["https://storage.jpostdb.org/JPST002084/files/PK20053_result_adlib.xlsx"],"Raw":["https://storage.jpostdb.org/JPST002084/files/051_41-4.raw","https://storage.jpostdb.org/JPST002084/files/033_3-3.raw","https://storage.jpostdb.org/JPST002084/files/052_43-4.raw","https://storage.jpostdb.org/JPST002084/files/067_42-5.raw","https://storage.jpostdb.org/JPST002084/files/018_5-2.raw","https://storage.jpostdb.org/JPST002084/files/002_4-1.raw","https://storage.jpostdb.org/JPST002084/files/068_44-5.raw","https://storage.jpostdb.org/JPST002084/files/017_2-2.raw","https://storage.jpostdb.org/JPST002084/files/034_6-3.raw","https://storage.jpostdb.org/JPST002084/files/001_1-1.raw"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Shinya Kuroda"],"species":["Mus Musculus (mouse)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST002084"],"submitter_affiliation":["University of Tokyo"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"name_synonyms":["Mus musculus, determination, Laboratory, Swiss, mice, Mus domesticus, mouse, Swiss Mouse, House Mice, Swiss Mice, House Mouse, domesticus, Laboratory Mice, jecur, iecur., Livers, House, Mus, chemical analysis, Mus musculus domesticus, Mouse, assay, house mouse, Mice, Laboratory Mouse"],"description_synonyms":["Water, sodium salt, MGC130048, IPP2A2, DmelCG8566, ammonium formate, Sodium Dodecyl, 13C-labeled, E 920, cadmium salt, MeCN, NCMe, determination, Laboratory, Mus domesticus, classic hairy cell leukaemia, Dodecyl Sulfate, H(2)O, zinc formate, A4, BOUND WATER, lead salt, CASP-14, SDH, Hydrogenchlorid, Basodexan, 2-(indol-3-yl)ethanoic acid, protein, magnesium formate, DmKlp53D, oxidane, mg/ml, House Mouse, zinc salt, CG11121, WATER, HOH, Bca, Alkylations, 5730420M11Rik, 2-Propanone, SDS, Polypeptides, 1H-imidazoleacetic acid, old, ammonium tetraformate, hydrogen chloride, IAA, peptido, cobalt(II) formate dihydrate, congenital, azanium, CH3-C#N, Min, AGM4, 2-amino-3-mercaptopropanoic acid, Cystein, protein aggregate, methyl ketone, DmelCG42273, insoluble, bca, gamma sarcoglycan, C, SET, peptides, TAF-I, cisteina, E927b, Tissue, iecur, min, Swiss Mice, Lauryl Sulfate, mAPC, copper, SO, lipomatosis of pancreas, EG:196F3.2, AI047805, DmelCG4299, IGAAD, set, IMAC, Wasserstoffchlorid, lithium salt, column, SOLO DANCERS, Propanon, DmelCG10574, scientific observation, gamma-sarcoglycan, dihydridooxygen, Shwachman-Bodian syndrome, Lyw-57, Half Cystine, dihydrogen oxide, imac, 1-(14)C-labeled., ammonium (2:1) salt, So, Ammonium(1+), nickel salt, phapii, Bonin Islands, Zinc Cysteinate, cobalt (+2) salt, dimethylketone, TSC-1, SG-gamma, CASP14, mouse, aqua, Dmel_CG7826, AI836084, StF-IT-1, classic hairy cell leukemia, KIF1B, Cys, ur, Aceton, SLP65, carbamide, H2O, Ly-57, NH4(+), (Indol-3-yl)acetate, sodium (4:1:1) salt, Pyroacetic ether, Dm1, magnesium salt, Mini-ICE, Chlorwasserstoff, sarcoglycan, IES, CG8566, CG14784, (2R)-2-amino-3-sulfanylpropanoic acid, nickel (+2) salt, Dmel_CG7835, Hydrogen Oxide, CG14783, Mnb, MNB, Mus musculus, imidazole-4-acetic acid hydrochloride, SLP-65, ammonium salt, HLA-DR-associated protein II, Caspase-14 subunit p10, BASH, DI-2, LY57, mice, I-2Dm, HCl, Swiss Mouse, formate, Shwachman-Diamond type metaphyseal dysplasia, hydrogen hydroxide, cobaltous formate, cromium (+3), Caspase-14 subunit p19, L-Cysteine, Shwachman syndrome, CG4299, AW124434, gamma (35kDa dystrophin-associated glycoprotein), MICE, beta-Ketopropane, beta Trypsin, acqua, L-Cystein, domesticus, I-2PP1, Irium, H2NC(O)NH2, DmCG8566, DMDA, heteroauxin, SCYA26, TAF-IBETA, 35kD dystrophin-associated glycoprotein, Acetamide, copper salt, ammonium (4:1) salt, Mouse, TAF-Ibeta, Wasser, Polypeptide, methyl cyanide, chlorane, DmelCG32810, lead (+2) salt, i2pp2a, 1728, glutamic acid side-chain, 3.4.22.-, F10B6_15, Ammonium, Dunc104, Cysteine Hydrochloride, SGCG_HUMAN, nickel formate dihydrate, lead formate, chlorure d'hydrogene, Sodium Lauryl, aluminum salt, cesium salt, L-Zystein, Gene, mini-ICE, Carbamide, protein-containing complex, TYPE, [NH4](+), F10B6.15, CG7826, PHAPII, agua, jecur, formic acid, DAGA4, Karbamid, ammonium cation, propanone, potassium salt, DmelCG11121, House, Ly57, 14C-labeled, Harnstoff, 35DAG, dimethylcetone, CG7835, Gene Products, Mus musculus domesticus, CG42273, Half-Cystine, Carmol, MAM, gamma-SG, SCG3, Mice, PH3, cloruro de hidrogeno, somda, anatomical systems, Hydrogen chloride, thallium (+1) salt, Swiss, L Cysteine, E-920, ammonium ion, ipp2a2, beta-Trypsin, rubidium salt, phosphane, imidazolyl-4-acetic acid, 4733401P19Rik, blocked, 2pp2a, Indoleacetic acid, Hcys, eau, calcium formate, methanoic acid, CG10574, Solution, lysate, 2PP2A, (indol-3-yl)acetic acid, MIP-4a, CYSTEINE, taf-ibeta, congenital lipomatosis of pancreas, dSET, dSet, FREE CYSTEINE, peptidos, cromium (+3) salt, Azeton, (2R)-2-amino-3-mercaptopropanoic acid, Dimethyl ketone, 2-iodo-, sodium formate, TCEP, BLNK-S, Drl, measuring, Sulfuric acid monododecyl ester sodium salt, DYRK1, Dimethylketon, nickel formate, 35 kDa dystrophin-associated glycoprotein, 3H-labeled, protein complex, Proteins, imidazole-4-acetic acid, igaad, strontium formate, NH4+, chloridohydrogen, potassium formate, copper (+2) salt, strontium salt, acetonitrile, Peptide, SGCG, Mdu, LGMD2C, group, [HCl], Sodium Lauryl Sulfate, Phosphopeptide, Temperatures, native protein, Mus, carbonyldiamide, I-2PP2A, mda, Unc104, water, cupric formate, chemical analysis, Protein, Dm I-2, I2PP2A, Hydrochloride, Sodium, Dyrk1, aluminum formate, ACETONITRILE, ami, Zystein, cyanomethane, AU020952, lithium formate, PRSS, ammonium, DMDA1, ensemble, uree, 2-amino-3-sulfanylpropanoic acid, med, CC1, 3-Indolylessigsaeure, Tripcellim, SWDS, L-2-Amino-3-mercaptopropionic acid, hydrogen phosphorus, House Mice, Shwachman-Bodian-Diamond syndrome, MIP-4alpha, (R)-2-amino-3-mercaptopropanoic acid, Klp53D, ethanenitrile, CGI-97, 2-Amino-3-mercaptopropionic acid, Unc-104, chromic formate, Laboratory Mice, ME-IV, Protein Gene Products, Livers, Gene Proteins, Trypure, dSET/TAF-Ibeta, 2610030F17Rik, hydrogen phosphide, concentration, Pancreatic insufficiency and bone marrow dysfunction, 2-carboxyethyl, SCARMD2, calcium salt, E920, Peptid, assay, [OH2], AA407739, Laboratory Mouse, Sulfate, CG32810, hydrochloric acid"],"additional_accession":[]},"is_claimable":false,"name":"Phosphoproteome analysis of mouse liver at ad libitum feeding","description":"The liver samples were collected from ad libitum feeding 10-week-old wild-type and ob/ob mice. Tissues were lysed with 0.5 mL of a solution containing 50 mM Tris-HCl pH8.8, 2% sodium dodecyl sulfate (SDS), and 7 M urea, and then homogenized by ultrasonication using a Bioruptor (Digaenode, Denville, NJ, USA). After dilution with equal amount of water, the samples were centrifuged at 15,000 g for 15 min at 4°C to exclude the insoluble fractions. Bicinchoninic acid (BCA) assay (Thermo Fisher Scientific) was used to quantify the protein concentration of the lysates, and lysate concentration was adjusted to 1 mg/mL. Cysteine residues were blocked using 2 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Thermo Fisher Scientific) at 37 °C for 30 min followed by alkylation with 10 mM 2-iodoacetamide (IAA) at room temperature for 30 min. Proteins were precipitated by addition of acetone. The resulting protein pellet was digested with trypsin in 100 mM ammonium bicarobonate. To enrich the phosphopeptides, the resulting peptides (1 mg) were subjected to Fe3+-IMAC. All samples were analyzed with Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) equipped with the UltiMate 3000 RSLCnano LC system (Thermo Fisher Scientific) via a nano-electrospray source with a column oven set at 60°C (AMR Inc.). Peptides were directly injected onto a 75 μm × 120 mm column (Nikkyo Technos Co.,Ltd., Tokyo, Japan) with a linear gradient of 5–32% B for 70 min and 32–65% B for 10 min at a flow rate of 200 nL/min, where A is 0.1% formic acid and B is 0.1% formic acid and 80% acetonitrile.","dates":{"publication":"Fri Jan 12 00:00:00 GMT 2024"},"accession":"PXD048480","cross_references":{"TAXONOMY":["10090"]}}