{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Tabular":["https://storage.jpostdb.org/JPST002083/files/20210926_result_peak_transfer.pr_matrix_LH_ProteinRatio_med_adlib.tsv","https://storage.jpostdb.org/JPST002083/files/20210926_result_peak_transfer.pr_matrix_adlib.tsv"],"Raw":["https://storage.jpostdb.org/JPST002083/files/KK0442_41.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_04.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_43.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_03.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_42.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_02.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_06.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_05.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_01.raw","https://storage.jpostdb.org/JPST002083/files/KK0442_44.raw"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Shinya Kuroda"],"species":["Mus Musculus (mouse)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST002083"],"submitter_affiliation":["University of Tokyo"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"name_synonyms":["Mus musculus, determination, Laboratory, Swiss, mice, Mus domesticus, mouse, Swiss Mouse, total expressed protein, House Mice, Swiss Mice, House Mouse, domesticus, Laboratory Mice, jecur, iecur., Livers, House, Mus, chemical analysis, Mus musculus domesticus, Mouse, assay, house mouse, Mice, Proteomes, Laboratory Mouse"],"description_synonyms":["Water, MGC130048, Sodium Dodecyl, E 920, determination, Laboratory, AI326280, Mus domesticus, classic hairy cell leukaemia, Dodecyl Sulfate, H(2)O, A4, BOUND WATER, CASP-14, SDH, Hydrogenchlorid, Basodexan, protein, 2-(indol-3-yl)ethanoic acid, oxidane, mg/ml, House Mouse, CG11121, WATER, HOH, Bca, Alkylations, peptide, 2-Propanone, SDS, Polypeptides, old, hydrogen chloride, peptido, IAA, BANF, congenital, B1, Min, AGM4, 2-amino-3-mercaptopropanoic acid, Cystein, protein aggregate, methyl ketone, DmelCG42273, insoluble, bca, gamma sarcoglycan, 3-hydroxy-5-estrane-17-carbonitrile, C, C79325, peptides, K, cisteina, E927b, 2610036I19Rik, Tissue, 2610510L13Rik, iecur, epsilon-diaminocaproic acid, min, Swiss Mice, Lauryl Sulfate, mAPC, achromobacter beta-lytic protease (blp), purification, SO, lipomatosis of pancreas, AI047805, isolation and purification, fSAP152, Wasserstoffchlorid, LYS, Lys, SOLO DANCERS, Propanon, scientific observation, anatomical tube, Lysin, ACN, D1, Acn, gamma-sarcoglycan, dihydridooxygen, Shwachman-Bodian syndrome, Lyw-57, TEAB, Half Cystine, dihydrogen oxide, So, Lzm, Lzp, Tube, Bonin Islands, Zinc Cysteinate, dimethylketone, acid, SG-gamma, CASP14, mouse, aqua, Dmel_CG7826, AI836084, classic hairy cell leukemia, Cys, ur, Aceton, SLP65, TUBE, carbamide, H2O, Ly-57, (Indol-3-yl)acetate, Pyroacetic ether, Dm1, Mini-ICE, Chlorwasserstoff, lysyl endoprotease, sarcoglycan, Lzm-s1, IES, (2R)-2-amino-3-sulfanylpropanoic acid, Dmel_CG7835, Hydrogen Oxide, Mnb, MNB, Mus musculus, Acid, SLP-65, Caspase-14 subunit p10, BASH, LY57, acide., mice, HCl, Swiss Mouse, Shwachman-Diamond type metaphyseal dysplasia, hydrogen hydroxide, acids, Caspase-14 subunit p19, L-Cysteine, alpha, Shwachman syndrome, acido, AW124434, gamma (35kDa dystrophin-associated glycoprotein), MICE, beta-Ketopropane, acqua, L-Cystein, domesticus, Irium, Lysm, H2NC(O)NH2, DMDA, heteroauxin, 35kD dystrophin-associated glycoprotein, Acetamide, duct, Mouse, Wasser, Polypeptide, chlorane, 1728, glutamic acid side-chain, 3.4.22.-, F10B6_15, Cysteine Hydrochloride, SGCG_HUMAN, chlorure d'hydrogene, Sodium Lauryl, L-Zystein, Gene, mini-ICE, Carbamide, ACINUS, protein-containing complex, 17alpha)-isomer, TYPE, F10B6.15, CG7826, agua, jecur, DAGA4, Karbamid, propanone, DmelCG11121, House, isolation, Ly57, Harnstoff, 35DAG, dimethylcetone, CG7835, Gene Products, Mus musculus domesticus, CG42273, Half-Cystine, Carmol, MAM, gamma-SG, SCG3, Mice, PH3, cloruro de hidrogeno, somda, 2310061K05Rik, Acinus, Hydrogen chloride, Swiss, L Cysteine, E-920, phosphane, 4733401P19Rik, blocked, Indoleacetic acid, Hcys, eau, Solution, acinusL, (indol-3-yl)acetic acid, CYSTEINE, Suspension, acinusS, congenital lipomatosis of pancreas, FREE CYSTEINE, peptidos, Azeton, (2R)-2-amino-3-mercaptopropanoic acid, Dimethyl ketone, Saeure, 2-iodo-, TCEP, PTHB1, BLNK-S, Drl, Lyzs, measuring, Sulfuric acid monododecyl ester sodium salt, DYRK1, Dimethylketon, 35 kDa dystrophin-associated glycoprotein, protein complex, Proteins, chloridohydrogen, Peptide, mKIAA0670, SGCG, Mdu, LGMD2C, polypeptide, [HCl], Sodium Lauryl Sulfate, native protein, Temperatures, Mus, carbonyldiamide, mda, C18, water, chemical analysis, Protein, AI551343, Hydrochloride, 5alpha, Sodium, Dyrk1, (3alpha, dJ142L7.2, ami, Zystein, Achromobacter protease I, AU020952, DMDA1, uree, 2-amino-3-sulfanylpropanoic acid, Saeuren, med, CC1, 3-Indolylessigsaeure, SWDS, L-2-Amino-3-mercaptopropionic acid, hydrogen phosphorus, House Mice, Shwachman-Bodian-Diamond syndrome, (R)-2-amino-3-mercaptopropanoic acid, CGI-97, 2-Amino-3-mercaptopropionic acid, Laboratory Mice, ME-IV, Protein Gene Products, achromopeptidase, Livers, Gene Proteins, hydrogen phosphide, concentration, Pancreatic insufficiency and bone marrow dysfunction, 2-carboxyethyl, SCARMD2, E920, Peptid, assay, [OH2], SCH, Laboratory Mouse, Sulfate, hydrochloric acid, 3-OHECN"],"additional_accession":[]},"is_claimable":false,"name":"Proteome analysis of mouse liver at ad libitum feeding","description":"The liver samples were collected from ad libitum feeding and Lys(6)-SILAC (Silantes, München, Germany) 10-week-old wild-type and ob/ob mice. Tissues were lysed with 0.5 mL of a solution containing 50 mM Tris-HCl pH8.8, 2% sodium dodecyl sulfate (SDS), and 7 M urea, and then homogenized by ultrasonication using a Bioruptor (Digaenode, Denville, NJ, USA). After dilution with water, the samples were centrifuged at 15,000 g for 15 min at 4°C to exclude the insoluble fractions. By calibrating to 1 mg/mL, bicinchoninic acid (BCA) assay (Thermo Fisher Scientific) was used to quantify the protein concentration of the lysates. Then, 50 µg of non-labeled and labeled protein extracts were combined in another tube for peptide preparation. We blocked cysteine residues using 2 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Thermo Fisher Scientific) at 37 °C for 30 min followed by alkylation with 10 mM 2-iodoacetamide (IAA) at room temperature for 30 min. By ultrasonically treating the pellet three times for 30 s with intervals of 30 s with a Bioruptor (Diagenode), the proteins precipitated with acetone for 3 h at −30 °C were dispersed in 50 mM triethylammonium bicarbonate. Lysyl endopeptidase (Wako, Osaka, Japan) was added to the protein suspension and digested at 37 °C for 16 h. Prior to MS analysis, the resulting peptides were purified using C18-StageTip purification after centrifuging at 15,000 g for 15 min at 4°C. Before MS analysis, peptides were resolved in 50 µL 3% ACN-0.1% foromic acid.","dates":{"publication":"Fri Jan 12 00:00:00 GMT 2024"},"accession":"PXD048481","cross_references":{"TAXONOMY":["10090"]}}