{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Csv":["https://storage.jpostdb.org/JPST003237/files/Peptides.csv","https://storage.jpostdb.org/JPST003237/files/Protein-Groups.csv"],"Raw":["https://storage.jpostdb.org/JPST003237/files/NA-12_p53OK_Tet-on_p53WT_Normal_2.raw","https://storage.jpostdb.org/JPST003237/files/NA-15_p53OK_Tet-on_p53R280K_Low-Choleterol_1.raw","https://storage.jpostdb.org/JPST003237/files/NA-09_p53OK_Tet-on_p53R280K_Normal_1.raw","https://storage.jpostdb.org/JPST003237/files/NA-16_p53OK_Tet-on_p53R280K_Low-Choleterol_2.raw","https://storage.jpostdb.org/JPST003237/files/NA-11_p53OK_Tet-on_p53WT_Normal_1.raw","https://storage.jpostdb.org/JPST003237/files/NA-10_p53OK_Tet-on_p53R280K_Normal_2.raw","https://storage.jpostdb.org/JPST003237/files/NA-21_p53OK_Tet-on_p53R280K_High-Choleterol_1.raw","https://storage.jpostdb.org/JPST003237/files/NA-22_p53OK_Tet-on_p53R280K_High-Choleterol_2.raw"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Tomoaki Tanaka"],"species":["Homo Sapiens (human)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST003237"],"submitter_affiliation":["Kazusa DNA Research Institute"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Proteomics analysis of differentially expressed proteins by p53 status and cholesterol concentration of culture medium in on-top 3D culture using MDA-MB-231","description":"Tumor cells had p53 knocked down by CRISPR/Cas9. In addition, Doxycycline-induced p53WT or p53R280K was incorporated into the p53KO cells by the Tet-on system. Furthermore, we created a high cholesterol condition in that Cholesterol was added to the culture medium while p53R280K was being expressed, and a low cholesterol condition in which Cholesterol was reduced by reducing serum.　The cells were cultured for 7 days in on-top 3D with respect to these four conditions. Whole cell lysates were processed for non-targeted proteomic analysis.","dates":{"publication":"Sun Sep 14 00:00:00 BST 2025"},"accession":"PXD054373","cross_references":{"TAXONOMY":["9606"]}}