JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST003237/files/Peptides.csvhttps://storage.jpostdb.org/JPST003237/files/Protein-Groups.csvhttps://storage.jpostdb.org/JPST003237/files/NA-12_p53OK_Tet-on_p53WT_Normal_2.rawhttps://storage.jpostdb.org/JPST003237/files/NA-15_p53OK_Tet-on_p53R280K_Low-Choleterol_1.rawhttps://storage.jpostdb.org/JPST003237/files/NA-09_p53OK_Tet-on_p53R280K_Normal_1.rawhttps://storage.jpostdb.org/JPST003237/files/NA-16_p53OK_Tet-on_p53R280K_Low-Choleterol_2.rawhttps://storage.jpostdb.org/JPST003237/files/NA-11_p53OK_Tet-on_p53WT_Normal_1.rawhttps://storage.jpostdb.org/JPST003237/files/NA-10_p53OK_Tet-on_p53R280K_Normal_2.rawhttps://storage.jpostdb.org/JPST003237/files/NA-21_p53OK_Tet-on_p53R280K_High-Choleterol_1.rawhttps://storage.jpostdb.org/JPST003237/files/NA-22_p53OK_Tet-on_p53R280K_High-Choleterol_2.rawprimaryOK200ProteomicsTomoaki TanakaHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST003237Kazusa DNA Research InstitutejPOSTfalseProteomics analysis of differentially expressed proteins by p53 status and cholesterol concentration of culture medium in on-top 3D culture using MDA-MB-231Tumor cells had p53 knocked down by CRISPR/Cas9. In addition, Doxycycline-induced p53WT or p53R280K was incorporated into the p53KO cells by the Tet-on system. Furthermore, we created a high cholesterol condition in that Cholesterol was added to the culture medium while p53R280K was being expressed, and a low cholesterol condition in which Cholesterol was reduced by reducing serum.　The cells were cultured for 7 days in on-top 3D with respect to these four conditions. Whole cell lysates were processed for non-targeted proteomic analysis.Sun Sep 14 00:00:00 BST 2025PXD0543739606