JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D_BP_MK_009_5_T2_5_protein.tsvhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D_BP_MK_009_2_C2_2_protein.tsvhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D_BP_MK_009_1_C1_1_protein.tsvhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D_BP_MK_009_6_T3_6_protein.tsvhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D_BP_MK_009_4_T1_4_protein.tsvhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D_BP_MK_009_3_C3_3_protein.tsvhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009_6-T3.mzMLhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009_2-C2.mzMLhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009_3-C3.mzMLhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009_4-T1.mzMLhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009_5-T2.mzMLhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009_1-C1.mzMLhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009%233.wiff.scanhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009%236.wiffhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009%234.wiffhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009%234.wiff.scanhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009%235.wiffhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009%235.wiff.scanhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009%236.wiff.scanhttps://storage.jpostdb.org/JPST003579/files/20230227_TT5600_D-BP-MK-009%233.wiffprimaryOK200ProteomicsMasaru KakuHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST003579Niigata UniversityjPOSTfalseChemical Digestion-Assisted Proteomic Analysis of Extracellular Matrix Profile of Human Periodontal LigamentThe extracellular matrix (ECM) plays a crucial role in tissue structure and function and serves as an integral component of diverse biological systems. However, the comprehensive characterization of ECM components is challenging because of the insoluble nature of highly cross-linked and glycosylated ECM proteins. This study introduces a chemical digestion-assisted proteomic approach to overcome challenges in ECM profiling, offering an in-depth analysis of the human periodontal ligament (PDL), a tissue critical for oral function and regeneration. Furthermore, we investigated alterations in the ECM composition of cultured PDL-derived cells to provide insights relevant to tissue engineering applications. Our protocol combined chemical digestion and deglycosylation to enhance the identification of ECM proteins. Sun Jul 12 00:00:00 GMT+01:00 2026PXD0603459606