JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R2_F5.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R1_F5.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R2_F7.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R2_F3.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R1_F3.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R1_F2.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R1_F7.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R2_F2.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R2_F4.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R1_F6.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R2_F6.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R1_F4.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R2_F1.rawhttps://storage.jpostdb.org/JPST003714/files/20230716_Eclipse_VQ_ZS_ZL_HPG_dss_tmt6_R1_F1.rawhttps://storage.jpostdb.org/JPST003714/files/20210705_Uniprot_human_reviewed_isoforms.fastahttps://storage.jpostdb.org/JPST003714/files/20230724_ZS_ZL_MaxQuant_HPG_dss_tmt6_7fractions_set1.ziphttps://storage.jpostdb.org/JPST003714/files/20230724_ZS_ZL_MaxQuant_HPG_dss_tmt6_7fractions_labelswap.zipprimaryOK200ProteomicsZhewang LinHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST003714IMCB A-STAR SingaporejPOSTfalseGlobal profiling of nascent chain interactors reveals TRIM25 as a novel co-translational E3 ubiquitin ligaseNascent polypeptide chains emerging from the ribosome engage a range of co-translational factors at distinct phases of translation. These co-translational interactions are crucial for proper protein biogenesis and quality control pathways to maintain protein homeostasis. Hence, systematic identification of these co-translational interactors provides insights into how distinct polypeptide fates are determined. Here, we developed Nascent Chain Interactor Profiling (NCIP), a metabolic labelling and chemical crosslinking enabled proteomics method to identify proteins interacting with nascent polypeptide chains at a proteome-wide scale. Results from NCIP support a widespread mechanism of co-translational assembly of protein complexes and reveal TRIM25 as a novel co-translational E3 ubiquitin ligase. TRIM25 ubiquitinates misfolded nascent polypeptide chains for quality control at the ribosome. Our results provide a generalisable framework to systematically profile nascent polypeptide chains interactors. Thu Mar 19 00:00:00 GMT 2026PXD0620199606