JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST003738/files/11-HCC_32.wiff.scanhttps://storage.jpostdb.org/JPST003738/files/2-HCC_2.wiffhttps://storage.jpostdb.org/JPST003738/files/11-HCC_33.wiffhttps://storage.jpostdb.org/JPST003738/files/13-HCC_4.wiff.scanhttps://storage.jpostdb.org/JPST003738/files/13-HCC_4.wiff2https://storage.jpostdb.org/JPST003738/files/11-HCC_33.wiff.scanhttps://storage.jpostdb.org/JPST003738/files/2-HCC_2.wiff.scanhttps://storage.jpostdb.org/JPST003738/files/13-HCC_4.wiffhttps://storage.jpostdb.org/JPST003738/files/11-HCC_33.wiff2https://storage.jpostdb.org/JPST003738/files/1-HCC_1.wiffhttps://storage.jpostdb.org/JPST003738/files/11-HCC_3.wiffhttps://storage.jpostdb.org/JPST003738/files/15-HCC_5.wiff2https://storage.jpostdb.org/JPST003738/files/11-HCC_32.wiff2https://storage.jpostdb.org/JPST003738/files/11-HCC_3.wiff2https://storage.jpostdb.org/JPST003738/files/15-HCC_5.wiff.scanhttps://storage.jpostdb.org/JPST003738/files/1-HCC_1.wiff2https://storage.jpostdb.org/JPST003738/files/11-HCC_32.wiffhttps://storage.jpostdb.org/JPST003738/files/1-HCC_1.wiff.scanhttps://storage.jpostdb.org/JPST003738/files/11-HCC_3.wiff.scanhttps://storage.jpostdb.org/JPST003738/files/2-HCC_2.wiff2primaryOK200ProteomicsSumio OhtsukiHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST003738Kumamoto UniversityjPOSTfalseFFPE proteomics for hepatocellular carcinomaFormalin-fixed paraffin-embedded (FFPE) tissues are crucial clinical archives linked with long-term follow-up data, yet the suitability of deep proteomic analysis on samples stored over 30 years remains largely unexplored. This study aimed to verify the feasibility of deep proteomic analysis on extremely long-term stored FFPE samples. We employed adaptive focused acoustics (AFA) technology for efficient protein extraction, combined with SP3 cleanup and data-independent acquisition (DIA) mass spectrometry using a ZenoTOF 7600, to analyze FFPE samples of hepatocellular carcinoma (HCC) and adjacent non-tumor liver (NTL) tissues from 19 HCC patients from 1988-1992.Wed Apr 01 00:00:00 GMT+01:00 2026PXD0624569606