{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xlsx":["https://storage.jpostdb.org/JPST003777/files/Rawfile_list_NuclearProteome_DIA.xlsx"],"Tabular":["https://storage.jpostdb.org/JPST003777/files/report.pr_matrix.tsv","https://storage.jpostdb.org/JPST003777/files/report.pg_matrix.tsv"],"Raw":["https://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_8Cnuc_02.raw","https://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_8Cnuc_01.raw","https://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_2Cnuc_01.raw","https://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_2Cnuc_02.raw","https://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_4Cnuc_02.raw","https://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_4Cnuc_01.raw"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Masaki Matsumoto"],"species":["Mus Musculus (mouse)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST003777"],"submitter_affiliation":["Niigata University"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Nuclear proteome analysis of mouse preimplantation embryos","description":"Mouse embryos at the 2-cell to 8-cell stages were collected and treated with acidic Tyrode’s solution to remove the zona pellucida. Subsequently, embryos were split into single blastomeres, and nuclei were isolated from each blastomere using a micromanipulator. Proteomic samples were then prepared using the in situ digestion of alcohol-fixed cells (iSDAC) method with 50 (2-cell), 216 (4-cell), and 280 (8-cell) nuclei per sample. The nuclear proteome during mouse preimplantation development was identified by label-free quantification.","dates":{"publication":"Fri Apr 24 00:00:00 GMT+01:00 2026"},"accession":"PXD063271","cross_references":{"TAXONOMY":["10090"]}}