JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST003777/files/Rawfile_list_NuclearProteome_DIA.xlsxhttps://storage.jpostdb.org/JPST003777/files/report.pr_matrix.tsvhttps://storage.jpostdb.org/JPST003777/files/report.pg_matrix.tsvhttps://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_8Cnuc_02.rawhttps://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_8Cnuc_01.rawhttps://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_2Cnuc_01.rawhttps://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_2Cnuc_02.rawhttps://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_4Cnuc_02.rawhttps://storage.jpostdb.org/JPST003777/files/20220623_EVO40SPD_FAIMS45_15uDIA_4Cnuc_01.rawprimaryOK200ProteomicsMasaki MatsumotoMus Musculus (mouse)https://repository.jpostdb.org/entry/JPST003777Niigata UniversityjPOSTfalseNuclear proteome analysis of mouse preimplantation embryosMouse embryos at the 2-cell to 8-cell stages were collected and treated with acidic Tyrode’s solution to remove the zona pellucida. Subsequently, embryos were split into single blastomeres, and nuclei were isolated from each blastomere using a micromanipulator. Proteomic samples were then prepared using the in situ digestion of alcohol-fixed cells (iSDAC) method with 50 (2-cell), 216 (4-cell), and 280 (8-cell) nuclei per sample. The nuclear proteome during mouse preimplantation development was identified by label-free quantification.Fri Apr 24 00:00:00 BST 2026PXD06327110090