JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST003779/files/211216AM03_aMb_RT_Src(+)_1.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM14_aMb_enrich_52C_2.rawhttps://storage.jpostdb.org/JPST003779/files/211216AM30_aMb_RT_Src(-)_3.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM11_aMb_enrich_47C_3.rawhttps://storage.jpostdb.org/JPST003779/files/211216AM16_aMb_RT_Src(-)_2.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM17_aMb_enrich_57C_1.rawhttps://storage.jpostdb.org/JPST003779/files/211216AM17_aMb_RT_Src(+)_2.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM05_aMb_enrich_42C_1.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM02_aMb_enrich_37C_2.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM19_aMb_enrich_57C_3.rawhttps://storage.jpostdb.org/JPST003779/files/211216AM02_aMb_RT_Src(-)_1.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM06_aMb_enrich_42C_2.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM03_aMb_enrich_37C_3.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM15_aMb_enrich_52C_3.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM18_aMb_enrich_57C_2.rawhttps://storage.jpostdb.org/JPST003779/files/211216AM31_aMb_RT_Src(+)_3.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM01_aMb_enrich_37C_1.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM09_aMb_enrich_47C_1.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM10_aMb_enrich_47C_2.rawhttps://storage.jpostdb.org/JPST003779/files/220312AM07_aMb_enrich_42C_3.rawprimaryOK200ProteomicsYasushi IshihamaHomo Sapiens (human)Equus Caballushttps://repository.jpostdb.org/entry/JPST003779Kyoto UniversityjPOSTfalseDetecting protein higher-order structural changes using kinase as a phospho-labelerWe developed an in vitro kinase-based approach to detect proteome-wide protein conformational changes. By combining residue-specific phosphorylation with phosphopeptide enrichment, this method enables sensitive, site-specific structural profiling. We demonstrated that phosphorylation efficiency reflected structural differences and revealed conformational changes upon RNA digestion across the proteome.Mon Apr 27 00:00:00 BST 2026PXD06336997969606