JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%239.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%237.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%235.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%2311.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%232.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%234.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%236.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%231.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%2310.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%238.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%233.rawhttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%238.dathttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%2310.dathttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%231.dathttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%233.dathttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%239.dathttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%237.dathttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%232.dathttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%234.dathttps://storage.jpostdb.org/JPST003818/files/orb_151013_A-MM-209_%2311.datprimaryOK200ProteomicsTakeya NakagawaMus Musculus (mouse)https://repository.jpostdb.org/entry/JPST003818Nagasaki UniversityjPOSTfalseIdentification of SOX2 associated protein complexes in mouse ES cellsTo uncover novel components of the SOX2-associated protein complex, we established E14 mouse embryonic stem (ES) cells stably expressing Flag-HA-tagged SOX2. Whole-cell extracts were prepared and subjected to tandem affinity purification, sequentially using anti-Flag M2 and anti-HA agarose resins . The purified SOX2 complex was separated by SDS-PAGE and visualized by silver staining . Distinct protein bands specifically enriched in the SOX2 immunoprecipitate were excised and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) . Sat May 23 00:00:00 BST 2026PXD06422310090