{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Raw":["https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240511_MSQC_2.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240509_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240505_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240501_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240511_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_B1_CD8.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240510_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_B1_CD5.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240428_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240511_MSQC_1.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240504_MSQC_2.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240511_MSQC_3.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240503_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_B1_CD2.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240508_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240429_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_B1_CD4.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240512_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240502_MSQC.raw","https://storage.jpostdb.org/JPST003880/files/liulei_OE480_2024HCC_20240430_MSQC.raw"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Lei Liu; Ming Liang Ye"],"species":["Homo Sapiens (human)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST003880"],"submitter_affiliation":["Dalian Institute of Chemical Physics Chinese Academy of Sciences, Dalian 116023, China"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"pubmed_abstract":["Dysregulated protein glycosylation is a hallmark of cancer, and systematic investigation of glycosylation patterns is crucial for identifying biomarkers. However, current glycoproteomic studies are constrained by the limited quantification power of single MS files and focus on dysregulated glycopeptides while neglecting the underlying glycoproteins. To address this, we proposed a protein-centric strategy to prioritize proteins susceptible to aberrant glycosylation, aiming to uncover previously overlooked cancer-associated proteins. In this study, we analyzed 200 samples via quantitative glycoproteomics on an integrated platform. Notably, the Glyco-Decipher software's new match-between-run scheme was applied in a large-scale serum-based HCC cohort study to enhance single-shot intact glycopeptide profiling, boosting detection of significantly dysregulated site-specific glycans 4.8-fold compared to conventional method. The protein-centric strategy identified 26 glycoproteins, with Fibronectin emerging as a top diagnostic performer. Specifically, the N1007_H5N4S2 on Fibronectin exhibited excellent diagnostic performance for HCC, achieving an AUC value of 0.917. Furthermore, a machine learning model integrating N1007_H5N4S2 on Fibronectin and N107_H9N3 on Alpha-1-antitrypsin yielded AUC values of 0.950/0.973 (HCC), 0.976/0.922 (TNM-I HCC), and 0.948/0.867 (AFP-negative HCC) in two cohorts, respectively. These findings demonstrated the effectiveness of the protein-centric strategy in identifying robust biomarkers, highlighting the potential of site-specific glycans for improving HCC diagnosis."],"pubmed_title":["A Protein-Centric Strategy Coupled with Match-Between-Run Glycoproteomics Enables Discovery of Robust Site-Specific Glycan Biomarkers for Hepatocellular Carcinoma."],"pubmed_authors":["Liu Lei L, Ma Taiheng T, Liu Qi Q, Zhu He H, Fang Zheng Z, Ma Jiahong J, Yu Ting T, Wang Yan Y, Zhou Jiahua J, Liu Xiaoyan X, Li Yaqian Y, Guo Zhimou Z, Liang Xinmiao X, Dong Mingming M, Sun Deguang D, Ye Mingliang M"],"additional_accession":[]},"is_claimable":false,"name":"A protein-centric glycoproteomic strategy identifies site-specific glycans as robust diagnostic markers for hepatocellular carcinoma","description":" In this study, we achieved the quantitative glycoproteomics analysis of a large cohort of clinical specimens including 100 Hepatocellular carcinoma (HCC) and 100 healthy control human serums by using an integrated platform.","dates":{"publication":"Fri Jun 26 00:00:00 BST 2026"},"accession":"PXD065283","cross_references":{"TAXONOMY":["9606"],"pubmed":["41674345"]}}