{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xml":["https://storage.jpostdb.org/JPST003984/files/F008311%202025.1.29%C3%A5%E2%80%A0%C2%8D%C3%A8%C2%A7%C2%A3%C3%A6%C5%BE%C2%90%C3%A5%C2%BE%C5%92%20shOrai1.xml"],"Raw":["https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_13_shOrai_IF_1_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_15_shOrai_IF_3_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_16_shOrai_IF_4_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_3_shCTRL_3_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_12_shOrai_4_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_11_shOrai_3_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_6_shCTRL_IF_2_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_7_shCTRL_IF_3_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_2_shCTRL_2_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_9_shOrai_1_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_5_shCTRL_IF_1_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_8_shCTRL_IF_4_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_10_shOrai_2_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_4_shCTRL_4_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_14_shOrai_IF_2_re.raw","https://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_1_shCTRL_1_re.raw"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Masanari Umemura"],"species":["Homo Sapiens (human)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST003984"],"submitter_affiliation":["Yokohama city University"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"shCTRL and shOrai1, IFN-γ 6 hr","description":"We investigated whether store-operated Ca²⺠entry (SOCE) contributes to PD-L1 regulation in oral squamous cell carcinoma (OSCC) cells. Proteomic analysis revealed IFN-γ stimulation activates calcium-related signaling. Pharmacological SOCE inhibition with Synta66 suppressed IFN-γ-induced PD-L1 protein expression dose-dependently. Knockdown of Orai1 or STIM1 had no effect under basal conditions but significantly reduced PD-L1 induction under IFN-γ stimulation. Inhibition of CaMKII and CaMKKII also suppressed PD-L1 expression at both mRNA and protein levels. Furthermore, Orai1 or STIM1 knockdown OSCC cells exhibited reduced proliferation when co-cultured with CD8⺠T cells, suggesting SOCE inhibition enhances immune-mediated tumor suppression.","dates":{"publication":"Wed Aug 05 00:00:00 BST 2026"},"accession":"PXD066968","cross_references":{"TAXONOMY":["9606"]}}