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https://storage.jpostdb.org/JPST003984/files/F008311%202025.1.29%C3%A5%E2%80%A0%C2%8D%C3%A8%C2%A7%C2%A3%C3%A6%C5%BE%C2%90%C3%A5%C2%BE%C5%92%20shOrai1.xmlhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_13_shOrai_IF_1_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_15_shOrai_IF_3_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_16_shOrai_IF_4_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_3_shCTRL_3_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_12_shOrai_4_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_11_shOrai_3_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_6_shCTRL_IF_2_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_7_shCTRL_IF_3_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_2_shCTRL_2_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_9_shOrai_1_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_5_shCTRL_IF_1_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_8_shCTRL_IF_4_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_10_shOrai_2_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_4_shCTRL_4_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_14_shOrai_IF_2_re.rawhttps://storage.jpostdb.org/JPST003984/files/250114HF_Yamashita_1_shCTRL_1_re.rawprimaryOK200ProteomicsMasanari UmemuraHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST003984Yokohama city UniversityjPOSTfalseshCTRL and shOrai1, IFN-Î³ 6 hrWe investigated whether store-operated CaÂ²âº entry (SOCE) contributes to PD-L1 regulation in oral squamous cell carcinoma (OSCC) cells. Proteomic analysis revealed IFN-Î³ stimulation activates calcium-related signaling. Pharmacological SOCE inhibition with Synta66 suppressed IFN-Î³-induced PD-L1 protein expression dose-dependently. Knockdown of Orai1 or STIM1 had no effect under basal conditions but significantly reduced PD-L1 induction under IFN-Î³ stimulation. Inhibition of CaMKII and CaMKKII also suppressed PD-L1 expression at both mRNA and protein levels. Furthermore, Orai1 or STIM1 knockdown OSCC cells exhibited reduced proliferation when co-cultured with CD8âº T cells, suggesting SOCE inhibition enhances immune-mediated tumor suppression.Wed Aug 05 00:00:00 BST 2026PXD0669689606