{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xlsx":["https://storage.jpostdb.org/JPST004087/files/240325_Murano_PNUTS_Imputation_data.xlsx"],"Raw":["https://storage.jpostdb.org/JPST004087/files/143_PJ934_Murano_2_PNUTS25_plus_HA_IPMS_QEp_DIA.raw","https://storage.jpostdb.org/JPST004087/files/145_PJ934_Murano_3_PNUTS28_minus_HA_IPMS_QEp_DIA.raw","https://storage.jpostdb.org/JPST004087/files/141_PJ934_Murano_1_PNUTS25_minus_HA_IPMS_QEp_DIA.raw","https://storage.jpostdb.org/JPST004087/files/147_PJ934_Murano_4_PNUTS28_plus_HA_IPMS_QEp_DIA.raw"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Kensaku Murano"],"species":["Drosophila Melanogaster (fruit Fly)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST004087"],"submitter_affiliation":["Tokushima University"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"pubmed_abstract":["Transcriptional silencing of transposable elements (TEs) is guided by Piwi and PIWI-interacting RNAs (piRNAs) through the SFiNX complex to induce heterochromatin formation. We previously reported that members of the SFiNX complex can induce TE silencing prior to heterochromatin formation, suggesting that Piwi-mediated silencing can be separated into two steps: initiation and maintenance. While many studies have investigated the heterochromatin-dependent maintenance step of TE silencing, the mechanisms underlying silencing initiation remain elusive. In this study, we found that SFiNX can induce silencing independent of H1 and HP1a through an association between the SFiNX-interaction partner Sov and two proteins involved in transcription termination, phosphatase 1 nuclear targeting subunit (PNUTS) and Senataxin. Mechanistically, PNUTS and Senataxin affect the elongation speed or stalling of RNA polymerase II (RNA Pol II) to induce repression. Our findings suggest that piRNAs act as transcription termination signals in the initiation step of piRNA-guided transcriptional repression preceding heterochromatinization of TEs."],"pubmed_title":["The Piwi-piRNA complex initiates transposon silencing via transcription termination factors PNUTS and Senataxin."],"pubmed_authors":["Wu Yaning Y, Imai Rin R, Kosako Hidetaka H, Nakahara Toshiya T, Solberg Therese T, Siomi Haruhiko H, Murano Kensaku K"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic analysis of PNUTS-associated proteins immunopurified with an anti-HA antibody","description":"Immunoprecipitated proteins on beads were washed once and resuspended in 50 mM ammonium bicarbonate, followed by the addition of 200 ng trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, acidified, and desalted with GL-Tip SDB. Then, the eluates were evaporated and dissolved in 0.1% trifluoroacetic acid and 3% acetonitrile (ACN). The resultant peptides were analyzed by an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer in data-independent acquisition mode. Raw data were analyzed against an in silico spectral library generated from the UniProt database restricted to Drosophila melanogaster using DIA-NN 1.8.1. Missing values were imputed with random values sampled from the lower five percent of detected values.","dates":{"publication":"Fri Sep 19 00:00:00 BST 2025"},"accession":"PXD068513","cross_references":{"TAXONOMY":["7227"],"pubmed":["41167190"]}}