JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST004380/files/250217_sfc_holo_MS2_MS2_MS3_1500ng.csvhttps://storage.jpostdb.org/JPST004380/files/251022_sfc_holo_carD2_1500ng_MS2_MS2_MS3_IT05.csvhttps://storage.jpostdb.org/JPST004380/files/250217_sfc_holo_card__MS2_MS2_MS3_1500ng.csvhttps://storage.jpostdb.org/JPST004380/files/251023_sfc_holo2_1500ng_MS2_MS2_MS3_IT05.csvhttps://storage.jpostdb.org/JPST004380/files/251010_sfc_holo_carD2_1500ng_MS2_MS2_MS3_IT05.rawhttps://storage.jpostdb.org/JPST004380/files/250217_sfc_holo_MS2_MS2_MS3_1500ng.rawhttps://storage.jpostdb.org/JPST004380/files/250217_sfc_holo_card__MS2_MS2_MS3_1500ng.rawhttps://storage.jpostdb.org/JPST004380/files/251010_sfc_holo2_1500ng_MS2_MS2_MS3_IT05.rawhttps://storage.jpostdb.org/JPST004380/files/250217_sfc_holo_card__MS2_MS2_MS3_1500ng.fastahttps://storage.jpostdb.org/JPST004380/files/251023_sfc_holo2_1500ng_MS2_MS2_MS3_IT05.fastahttps://storage.jpostdb.org/JPST004380/files/251022_sfc_holo_carD2_1500ng_MS2_MS2_MS3_IT05.fastahttps://storage.jpostdb.org/JPST004380/files/250217_sfc_holo_MS2_MS2_MS3_1500ng.fastaprimary200OKProteomicsGeorgiy A. BelogurovSpirochaeta Africanahttps://repository.jpostdb.org/entry/JPST004380University of TurkujPOSTfalseSpirochaeta africana RNAP holoenzymeCrosslinking mass spectrometry was applied to investigate the localization of σR1.1 domain in the structure of Spirochaeta africana RNA polymerase holoenzyme. RNA polymerase holoenzymes were assembled using polymerase core subunits and σ factor heterologously expressed in Escherichia coli. Two complexes were assembled in the absence of S. africana CarD activator and two in the presence of CarD activator. The resulting four complexes were crosslinked using disuccinimidyl sulfoxide (DSSO). Crosslinked protein complexes were analyzed by LC-MS/MS, and crosslinked residue pairs were identified in the form of crosslinked peptides.Fri May 01 00:00:00 BST 2026PXD07404646355