{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fasta":["https://storage.jpostdb.org/JPST004613/files/Homo%20sapiens%20(SwissProt%20TaxID=9606).fasta"],"Other":["https://storage.jpostdb.org/JPST004613/files/raw.zip","https://storage.jpostdb.org/JPST004613/files/nn015_hase_139samples.pdResult"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Tetsunari Hase"],"species":["Homo Sapiens (human)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST004613"],"submitter_affiliation":["Nagoya University Hospital"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Serum proteome profiling of patients with non-small cell lung cancer treated with immune checkpoint blockade monotherapy","description":"Shotgun proteomic profiling of serum from patients with non-small cell lung cancer (NSCLC) treated with immune checkpoint blockade (ICB) monotherapy. Top-14 abundant proteins were depleted (Pierce High-Select, Thermo Fisher Scientific), and tryptic digests were spiked with the Pierce Peptide Retention Time Calibration Mixture as an internal standard. Peptides were analyzed by LC-MS/MS on an EASY-nLC 1200 coupled to an Orbitrap Eclipse Tribrid mass spectrometer with a FAIMS Pro interface (compensation voltages −40, −60, −80 V), using data-dependent acquisition with Orbitrap MS1 and ion-trap-detected HCD MS2. Raw files were searched in Proteome Discoverer 2.4 (SEQUEST HT with Percolator validation) against UniProt Swiss-Prot human (release 2017-10-25), and label-free protein abundances (Minora Feature Detector) were normalized to internal standard peptides. See the associated publication for full experimental details and clinical information.","dates":{"publication":"Wed Jun 10 00:00:00 BST 2026"},"accession":"PXD078247","cross_references":{"TAXONOMY":["9606"]}}