{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xlsx":["https://storage.jpostdb.org/JPST004713/files/26-034.xlsx"],"Raw":["https://storage.jpostdb.org/JPST004713/files/26-034.raw"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Hirotaka Watada"],"species":["Mus Musculus (mouse)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST004713"],"submitter_affiliation":["Laboratory of Proteomics and Biomolecular Science, Biomedical Research Core Facilities, Juntendo University Graduate School of Medicine"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Lipotoxicity impairs proinsulin processing by inhibiting the acidification of secretory granules by V-ATPase.","description":"ATP6V0d1 is a subunit of the vacuolar H+-ATPase (V-ATPase) complex and plays an important role in the regulation of secretory granule acidification and insulin processing in pancreatic β-cells. To identify the S-palmitoylation site of ATP6V0d1, proteomic analysis was performed using MIN6 cells.\nProtein S-palmitoylation was labeled using the RapidSPALM kit (BioDynamics), in which palmitoylated cysteine residues were substituted with MfTag. Proteins were separated by SDS-PAGE, and a gel band exhibiting an approximately 5 kDa upward shift, corresponding to MfTag-labeled ATP6V0d1, was excised and subjected to in-gel digestion followed by LC-MS/MS analysis. During sample preparation, MfTag-modified cysteine residues were converted to carbamidomethylated cysteines by iodoacetamide treatment. Peptides containing the modified cysteine residues were used to identify candidate S-palmitoylation sites.\nLC-MS/MS analysis identified a peptide containing modified Cys39, suggesting that Cys39 is a candidate S-palmitoylation site of ATP6V0d1.","dates":{"publication":"Thu Oct 01 00:00:00 GMT+01:00 2026"},"accession":"PXD080413","cross_references":{"TAXONOMY":["10090"]}}