{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xlsx":["https://storage.jpostdb.org/JPST004764/files/BY4741-vps35%20vs%20wt.xlsx","https://storage.jpostdb.org/JPST004764/files/BY4741-vps35.xlsx","https://storage.jpostdb.org/JPST004764/files/BY4741-wt.xlsx"],"Raw":["https://storage.jpostdb.org/JPST004764/files/BY4741-vps35.raw","https://storage.jpostdb.org/JPST004764/files/BY4741-wt.raw"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Prof. Jieqiong Gao"],"species":["Saccharomyces Cerevisiae (baker's Yeast)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST004764"],"submitter_affiliation":["HKUST"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Label-free vacuolar proteomics of wild-type and vps35Δ yeast using Orbitrap Eclipse","description":"Vacuoles were isolated from wild-type and vps35Δ S. cerevisiae cells grown to stationary phase. Peptides were digested using Preomics iST kit, analyzed by Thermo Orbitrap Eclipse in DDA mode with FAIMS (-45V/-65V). Data were searched against UniProt yeast database using Proteome Discoverer 2.4.1.15 with 10 ppm precursor and 0.02 Da fragment tolerances, filtered at 1% FDR. This dataset was used to identify retromer cargoes accumulating in vps35Δ vacuoles.","dates":{"publication":"Mon Jul 13 00:00:00 BST 2026"},"accession":"PXD080926","cross_references":{"TAXONOMY":["4932"]}}