JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST004764/files/BY4741-vps35%20vs%20wt.xlsxhttps://storage.jpostdb.org/JPST004764/files/BY4741-vps35.xlsxhttps://storage.jpostdb.org/JPST004764/files/BY4741-wt.xlsxhttps://storage.jpostdb.org/JPST004764/files/BY4741-vps35.rawhttps://storage.jpostdb.org/JPST004764/files/BY4741-wt.rawprimaryOK200ProteomicsProf. Jieqiong GaoSaccharomyces Cerevisiae (baker's Yeast)https://repository.jpostdb.org/entry/JPST004764HKUSTjPOSTfalseLabel-free vacuolar proteomics of wild-type and vps35Δ yeast using Orbitrap EclipseVacuoles were isolated from wild-type and vps35Δ S. cerevisiae cells grown to stationary phase. Peptides were digested using Preomics iST kit, analyzed by Thermo Orbitrap Eclipse in DDA mode with FAIMS (-45V/-65V). Data were searched against UniProt yeast database using Proteome Discoverer 2.4.1.15 with 10 ppm precursor and 0.02 Da fragment tolerances, filtered at 1% FDR. This dataset was used to identify retromer cargoes accumulating in vps35Δ vacuoles.Mon Jul 13 00:00:00 BST 2026PXD0809264932