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Ishihama"],"species":["Hela Cell"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST001715"],"submitter_affiliation":["jPOST"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"name_synonyms":["SNF1-RELATED PROTEIN KINASE 2-2, count in organism, SRK2D, SNRK2-2, SPK-2-2, SNF1-related protein kinase 2.2, Kinases, Protein, number, sequence, primary structure of sequence macromolecule., Kinase, PROTEIN KINASE, Protein Kinase, ATP-protein phosphotransferase, presence"],"description_synonyms":["glycogen synthase kinase activity, liquid chromatography tandem mass spectroscopy, 6353, Dmel_CG3263, protein kinase A activity, Activity, Product, determination, L-Threonine, CG18677, phosphorylation, brl, CG12452, Prp4 protein kinase activity, 2-Amino-3-hydroxypropionic acid, ATP-protein transphosphorylase activity, cAMP-dependent protein kinase, Pharmaceutical Product, ATP:protein phosphotransferase (non-specific) activity, Dpck, mitogen-activated protein kinase activity, Kinase, RI, Threonin, C, LCMSMS, pre-mortem, CG4379, Pk72A, 3', 5' cAMP-dependent protein kinase complex, CG15862, Raf kinase activity, project description., serine kinase activity, DmelCG6117, 5' cAMP-dependent protein kinase activity, 2-amino-3-hydroxypropanoic acid, atypical protein kinase C activity, medicine, Pharmaceutical, signaling process, mitogen-activated S6 kinase activity, protein-aspartyl kinase activity, SIMPLE, single organism signaling, p82 kinase activity, LC-MS2, betaIIPKC, cyclic AMP-dependent protein kinase complex, LC-MS/MS, CG42341, pka-C1, Expanded, M phase-specific cdc2 kinase activity, Wee-kinase activity, pka-R1, 3-Hydroxyalanine, MAPK, PIG7, 1270, 5'-cAMP-dependent protein kinase complex, Cos-1, RII[[DR]], Pharmaceutic, pka-c1, serine protein kinase activity, region, 5-23, PKA C, phosphorylase B kinase kinase activity, Epistemology, protein glutamyl kinase activity, expanded, CG6117, non-specific serine/threonine protein kinase activity, Ex, Dmel_CG12452, pka-RII, living, SNF1-RELATED PROTEIN KINASE 2-2, hydroxyalkyl-protein kinase activity, WEE1Hu, DmelCG4379, ATP:protein phosphotransferase (cAMP-dependent) activity, PKA RII, antagonists and inhibitors, enlarged, SNF1-related protein kinase 2.2, serine-specific protein kinase activity, liquid chromatography-tandem mass spectroscopy, dPKA, epsilon PKC, PkaC1, PKAcA, HIPK2, cytidine 3', l(2)ey, RII, L Serine, Epa, Wee 1-like kinase activity, L-Serine, Protein Kinase, Dcpk, adenosine 3', PKAC1, big, ribosomal S6 protein kinase activity, calcium/phospholipid-dependent protein kinase activity, biological signaling, glycogen synthase A kinase activity, glycogen synthase kinase 3 activity, Pk?5, PKA-C3, PKA-C-3, PKA-C1, BcDNA:GM01761, phosphorylase b kinase kinase activity, LC-MS-MS, large, DmelCG4114, Pka-RI, LC-MSMS, l(2)cos[[1]], serine(threonine) protein kinase activity, BG02142, AMPK, ribosomal protein S6 kinase II activity, 18304, T-antigen kinase activity, Drugs, galactosyltransferase-associated kinase activity, study, protein-serine kinase activity, Pka-C, anatomical systems, PKAR, PkaR, STK32, inhibiteur, PKa-R2, PKa-R1, L Threonine, PKAc, ATP-protein phosphotransferase, protein kinase (phosphorylating) activity, PKA-R1, DmelCG15862, 5'-cyclophosphate-dependent protein kinase activity, PKA-R2, drugs, SNRK2-2, Cos, inhibidor, CdkA, protein-cysteine kinase activity, Raf-1, great, Hpr kinase activity, Dc0, STK22, site, Preparation, casein kinase (phosphorylating) activity, DRI, PKA-RI, TP53I7, Pharmaceuticals, PKA Cl, inhibitors, Products, Cos1, 5'-cyclophosphate-dependent protein kinase complex, l(2)s4402, drug, DC0, CG4114, DC2, Phosphorylations, dPKA-RI, inhibitor, CG3263, CdkR, protein serine kinase activity, Cell, protein phosphokinase activity, cos1, Serin, twitchin kinase activity, LC/MS/MS, SRK2D, PKAi, cyclic AMP-dependent protein kinase activity, pkA, PKA, Pka, Phosphopeptide, PKAr, PKC, Protein, AP50 kinase activity, chemical analysis, dcO, DCO, Dco, intrinsic catalyst activity, protein kinase p58 activity, Pka-RII, serine/threonine protein kinase activity, antagonists, threonine-specific protein kinase activity, SPK-2-2, pka, pka C1, pKA, dr[[1]], PKA C3, Pharmaceutic Preparations, Kinases, dco, 5'-cAMP-dependent protein kinase activity, dsk1, PKA-RII, 5'-cyclic monophosphate-responsive protein kinase activity, atypical PKC activity, signalling, DmelCG42341, Drug, l(2)01270, PKA CI, Preparations, signalling process, l(2)01272, liquid chromatography tandem mass spectrometry, assay, Pharmaceutical Products, PROTEIN KINASE, protein serine-threonine kinase activity, General activity, A-kinase activity, Pharmaceutical Preparation"],"additional_accession":[]},"is_claimable":false,"name":"JPST000234-1 Identifications of protein kinase A substrates with quantitative phosphoproteomics and primary-sequence based scoring.(H-89) [Reanalysis: JPST000234]","description":"Protein kinase A (PKA) is a serine/threonine kinase who plays essential roles in the complex signalling pathways. To better understand the functionalities, it is necessary to elucidate direct interplays between PKA and their substrates in living system. In order to screen them in a high-throughput manner, we firstly quantified the change of phosphoproteome in the cells of which PKA activity was perturbed by drag stimulations. LC-MS/MS analysis identified 2,755 and 3,191 phosphopeptides from either experiments with activator or inhibitor of PKA. To exclude indirect targets of PKA, we built the simple model to characterize the kinase sequential specificity to its substrate recognitions based on the known kinase-substrate relationships. Finally, by applying the metric to the sites of which phosphorylation were changed due to the drug stimulations, we identified 29 reliable candidates of PKA targeting residues in living cells including 8 known substrates. Moreover, 18 sites out of them were confirmed to be site-specifically phosphorylated in vitro. Altogether this study proposed confident list of PKA substrate and expanded the knowledge of PKA signalling network. [Original project description]","dates":{"publication":"Sat Mar 25 00:00:00 GMT 2023"},"accession":"RPXD034682","cross_references":{"TAXONOMY":["CLO:0003684"]}}