{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Txt":["https://storage.jpostdb.org/JPST001725/files/150528aki_bw5.maxq.txt","https://storage.jpostdb.org/JPST001725/files/150528aki_bw5.txt","https://storage.jpostdb.org/JPST001725/files/rawfiles.txt"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Yasushi Ishihama"],"species":["Mus Musculus (mouse)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST001725"],"submitter_affiliation":["jPOST"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"description_synonyms":["Food Patterns, BODYFAT, CKIIb, Activity, methionine aminopeptidase activity, CK II, CKIIa, CK-IIbeta, Metabolisms, CK-2, Bioenergetics, Body fails to respond to insulin, phosphorylation, temporal, Adiposis, dCKII beta, Obese, unspecified, Abc8, response to cyclic AMP, Heat, 2, multicellular organism lipid catabolic process, Kinase, Expenditure, fatty tissue, animal, fat tissue, peptidase M activity, adenomas, signal transduction by protein phosphorylation, rabGAPLP, CK-II alpha, L-methionine aminopeptidase activity, bg, Occidental, adipose system, dCKII, anon-WO02059370.53, CK2-beta, hypoplasia, RabGAP-5, Food, project description., DmelCG17520, genetic, b, 5'-cAMP, DmelCG15224, RUSC3, Lipocytes, 5'-cyclophosphate, Nonshivering Thermogenesis, TRP-1, beige, CKIIalpha, isa, Tik, ATP Phosphotransferases, Insulin Sensitivity, Facultative Thermogenesis, ATP, CKII alpha, CKII beta, CK-II beta, AW413978, MYH-associated polyposis, CK2beta, familial, autosomal recessive familial adenomatous polyposis, signal transduction by conformational transition, Maps, white, Adaptive, results, DmCK2alpha, signal transduction by trans-phosphorylation, Fat Cells, Programs, multicellular organismal lipid catabolic process, signaling cascade, Body Fat, Ck2, brown, European, adp, CKII-alpha, Obesity [Ambiguous], Obese (finding), OBESITY, CK2-alpha, activation, autosomal recessive, Caucasians, Adipocyte, Oca3, RUTBC3, Transphosphorylases, l(3)S095808, DmCK2beta, CK2a, whole organism, CK2, ck2, Ds cas kin, DmCkIIbeta, Ckiibeta, alphaCK2, Adiposity, Heat Production, OBESITY NOS, fat cell, RABGAP5, DmCKIIalpha, Energy Metabolisms, Dietary Patterns, signaling pathway, Fat Cell, CK2alpha, Cells, multiple colorectal, Koerper, White, inherited genetic, ck2b, TRP1, Fat, Sympathins, CKIIbeta', 5' cAMP, CKIIb2, number, CkII-beta, obesity disease, Gm695, fat, presence, Patterns, CG8914, Sympathin, Obesity, Lipolyses, Caucasian, reduced, IR, Catecholamine, Metabolism, Insulin, Diets, CJIIalpha, tiny, Phosphotransferase, familial adenomatous polyposis, CKII-beta1, MAP, Thermogeneses, C77789, KC2B_DROME, Food Pattern, Facultative, CkIIbeta1, familial adenomatous polyposis 2, Having too much body fat, Energy, Overweight and obesity, Transphosphorylase, CG15224, Expenditures, CG17520, CKIIbeta, autosomal recessive multiple colorectal adenomas, Pattern, signalling pathway, Adaptive Thermogenesis, bodyfat, Lipocyte, Sensitivity, signal transduction by cis-phosphorylation, lipocyte, CK2 alpha, CK-II, Dietary Pattern, constitutitional genetic, incidence, D13Sfk13, small, mbu, ckIIalpha, MUTYH-Associated Polyposis, CkII, CKIIalfa, Tyrp, body, Phosphorylations, DmCKIIbeta', whole body, Production, CKII, Nonshivering, Cell, Phosphotransferases, obesity, count in organism, adipose cell, Drug resistance to insulin (disorder), 1110035J23Rik, DmelCG8914, Resistance, Thermogenesis, Ck-IIbeta, And, dCKII alpha, adipocyte, DmCK2beta', CCK2, signalling cascade, Caucasoid, obesity disorder, Obesity (disorder), Energy Expenditure, dCK2alpha, response to 3', response to adenosine 3', underdeveloped, Kinases, Dietary, Obesity NOS, betaCK2, Cask-II-a, Energy Expenditures, Cask-II-b, MYH-Associated Polyposis, Bioenergetic, CK2beta', fatty depot, Whites, adipose, DmCKIIbeta, Ck2alpha, MAP syndrome, dCK2beta, hereditary, General activity, FAP2, colorectal adenomatous polyposis, 0958/08"],"name_synonyms":["Brown Fat Cells, Adipocyte, Brown, brown adipose cell, Brown Fat, Fat Cell, Brown Adipocytes, Cells, brown fat cell, Brown Fat Cell., Brown Adipocyte, Cell, Fat Cells"],"additional_accession":[]},"is_claimable":false,"name":"JPST000097-2 Brown adipocyte phosphoproteomics [Reanalysis: JPST000097]","description":"Catecholamines promote lipolysis both in brown and white adipocytes, whereas the same stimuli preferentially activate thermogenesis in brown adipocytes. Molecular mechanisms for the adipose-selective activation of thermogenesis remain poorly understood. Here, we employed quantitative phosphoproteomics to map global and temporal phosphorylation profiles in brown, beige, and white adipocytes under β3-adrenenoceptor activation and identified kinases responsible for the adipose-selective phosphorylation profiles. We found that casein kinase2 (CK2) activity is preferentially higher in white adipocytes than brown/beige adipocytes. Genetic or pharmacological blockade of CK2 in white adipocytes activates the thermogenic program in response to cAMP stimuli. Such activation is largely through reduced CK2-mediated phosphorylation of class I HDACs. Notably, inhibition of CK2 promotes beige adipocyte biogenesis and leads to an increase in whole-body energy expenditure and ameliorates diet-induced obesity and insulin resistance. These results indicate that CK2 is a plausible target to rewire the β3-adrenenoceptor signaling cascade that promotes thermogenesis in adipocytes. [Original project description]","dates":{"publication":"Sat Mar 11 00:00:00 GMT 2023"},"accession":"RPXD034728","cross_references":{"TAXONOMY":["10090"]}}