JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST002907/files/rawfiles.txthttps://storage.jpostdb.org/JPST002907/files/mai100519_019HIPK2.txthttps://storage.jpostdb.org/JPST002907/files/mai100519_019HIPK2.maxq.txtprimaryOK200ProteomicsYasushi IshihamaHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST002907jPOSTjPOSTglycogen synthase kinase activity, ribosomal S6 protein kinase activity, scale tissue, calcium/phospholipid-dependent protein kinase activity, human being, protein kinase A activity, glycogen synthase A kinase activity, acetylglucosaminyltransferase-like protein, peltate hair, glycogen synthase kinase 3 activity, Mbp1, LARGE1, phosphorylase b kinase kinase activity, froggy, Gyltl1a, Prp4 protein kinase activity, Human, Homo sapiens, ATP-protein transphosphorylase activity, ATP:protein phosphotransferase (non-specific) activity, serine(threonine) protein kinase activity, mitogen-activated protein kinase activity, myd, ribosomal protein S6 kinase II activity, Man, T-antigen kinase activity, galactosyltransferase-associated kinase activity, 1110014O20Rik, protein-serine kinase activity, Man (Taxonomy), like-acetylglucosaminyltransferase, MDDGB6, plant peltate hair, STK32, Mbp-1, LARGE, Raf kinase activity, BPFD#36, protein kinase (phosphorylating) activity, serine kinase activity, protein-cysteine kinase activity, atypical protein kinase C activity, Raf-1, Hpr kinase activity, B230339E18Rik, mitogen-activated S6 kinase activity, protein-aspartyl kinase activity, casein kinase (phosphorylating) activity, Stank, p82 kinase activity, gyltl1b-b, Modern, betaIIPKC, protein serine kinase activity, protein phosphokinase activity, M phase-specific cdc2 kinase activity, Wee-kinase activity, PRO0593, twitchin kinase activity, PKA, PKC, MDDGA6, MAPK, mKIAA0609, AP50 kinase activity, scales, KIAA0609, serine protein kinase activity, acetylglucosaminyltransferase-like 1A, protein kinase p58 activity, serine/threonine protein kinase activity, fg, threonine-specific protein kinase activity, phosphorylase B kinase kinase activity, gyltl1b, scale, protein glutamyl kinase activity, mdc1d, non-specific serine/threonine protein kinase activity, dsk1, LARGE_HUMAN, human, 5'-cyclic monophosphate-responsive protein kinase activity, atypical PKC activity, MDC1D, hydroxyalkyl-protein kinase activity, WEE1Hu, like-glycosyltransferase, enr, serine-specific protein kinase activity, epsilon PKC, Modern Man, HIPK2, cytidine 3', Wee 1-like kinase activity, A-kinase activity., protein serine-threonine kinase activity, glycosyltransferase-like protein LARGE1Signal Transduction Systems, Signal Transductions, para Tyrosine, human being, Peptidomics, Receptor-Mediated, number, Signal Transduction System, L-Threonine, presence, Migrant Workers, Human, 2-Amino-3-hydroxypropionic acid, Receptor Mediated Signal Transduction, Signal Transduction, Homo sapiens, Kinase, Cell Signaling, Man, Phosphotransferase, Threonin, signal transduction by protein phosphorylation, Man (Taxonomy), Migrant Worker, Transients, 2-amino-3-(4-hydroxyphenyl)propanoic acid, 3-(p-Hydroxyphenyl)alanine, Nonmigrant, Signal, Pathways, Nonmigrants, Signal Pathways, L Threonine, Transphosphorylase, Worker, Y, Signal Transduction Pathway, Transient, project description., Squatter, set, signalling pathway, 2-amino-3-hydroxypropanoic acid, L Tyrosine, grupos, Tyr, Squatters, signal transduction by cis-phosphorylation, Signal Transduction Pathways, Transductions, Tyrosin, ATP Phosphotransferases, Signal Pathway, ATP, single organism signaling, Pathway, grupo, Modern, 2-Amino-3-(p-hydroxyphenyl)propionic acid, signal transduction by conformational transition, Receptor-Mediated Signal Transductions, total expressed protein, para-Tyrosine, Phosphotransferases, signal transduction by trans-phosphorylation, group, Tyrosine, signaling cascade, count in organism, Serin, 3-Hydroxyalanine, tirosina, Receptor-Mediated Signal Transduction, Migrant, Systems, Nomad, Workers, signalling cascade, Transduction, Migrants and Transients, Transphosphorylases, ensemble, Kinases, System, L-isomer, Rest, human, L isomer, signaling pathway, signalling process, Modern Man, Nomads, Migrants, L Serine, L-Tyrosine, L-Serine, Proteomes, groupe, GruppefalseLarge-scale Discovery of Substrates of the Human Kinome (HIPK2) [Reanalysis: JPST000508]Kinase -networks are important for cellular signal transduction. Despite tremendous efforts to uncover these signaling pathways, huge numbers of uncharacterized phosphosites still remain in the human proteome. Because of the transient nature of kinase-substrate interactions in vivo, it is almost impossible to identify direct substrates. Here, we present a novel strategy for the rapid and high-throughput discovery for of kinase substrates of kinase using quantitative proteomics. Using 385 purified kinases, we identified a total of 175,574 potential direct kinase substrates of kinases. In addition, we identified novel kinase groups, such as one group containing 30 threonine-directed kinases and another containing 15 serine/threonine/tyrosine kinase sgroup. Surprisingly, we observed that the diversity of substrates for tyrosine kinases was much higher than that for serine-threonine kinases. [Original project description]Wed Feb 21 00:00:00 GMT 2024RPXD0499279606