LINCSapplication/xmlhttp://lincsportal.ccs.miami.edu/dcic/api/download?path=LINCS_Data/HMS_LINCS&file=LDS-1088.tar.gzprimaryOK200MultiomicsQingsong Liu1U54HG006097-01KiNativMass spectrometryLifeHarmonizomeMLK3MK03DGKAARAFMYO3A,MYO3BABL,ARGPITSLRESLKTBK1NEK1MK11EPHB2M3K2,MAP3K3EPHB4MAP3K4MAP3K5p38aPDK1MAP2K3MAP2K4CaMK1aMARK2,MARK3CaMK2a,CaMK2b,CaMK2d,CaMK2gWnk1,Wnk2,Wnk4CASKLOKWnk1,Wnk2,Wnk3M4K3STLK5CK1d,CK1eeEF2KMST4,YSK1RSKL1_domain1MAPKAPK3MPSK1BTKULK3KS6B2KS6B1ULK1EPHA2RSK1 domain1MARK3,MARK4PRP4RSK1,RSK4 domain1WNK4MSK1_domain1IRAK1MRCKbIRE1PRPKJNK1,JNK2,JNK3MST1,MST2MARK2MAP2K6LYNNEK8NEK9DMPKMK14PI42CPLK1ACKBRAFMSK1,MSK2_domain1M3K2CLK2HPK1SRPK1CDK9CDK7FERJAK1_domain2MLTKNDR2CHK2PAN3CDK5CHK1KSR1,KSR2FESJAK2 domain2CDK2KHS1CDK10AurBPI4KBCDK14PKCa,PKCbATRCDK16GSK3BGSK3ASMG1MAST3MAP2K1,MAP2K2ILKPIK3CBPKD2PIK3CGErk5TAO1,TAOK3TLK2TLK1PKD1,PKD2JAK1MST2GCN2MST3CAMK1DIKKE,TBK1RIPK3SYKMST1CDK11,CDK8KPCIIRAK3IRAK4KC1AMASTLJAK3 domain2LATS1RONLATS2ZC1/HGK,ZC2/TNIK,ZC3/MINKPKRROCK1,ROCK2PHKg2BARK1MAST2,MAST4PCTAIRE2PIK3C3SGK3METRSK1,RSK2_domain1ROCK1SRCEphA1LKB1EGFRDNAPKEphA7p70S6K,p70S6KbNEK6,NEK7CSKIKKAAMPKa1,AMPKa2GCN2_domain2CaMK2gIKKEANPbp38d,p38gMAP3K1AGKSRPK1/2GCKFAKPI4KA,PI4KAP2RSK2_domain2SNRKWEE1RSK2_domain1TECPEKTAOK2FYN,SRC,YESPKN1MAP3K5,MAP3K6,MAP3K7http://lincsportal.ccs.miami.edu/datasets/#/view/LDS-1088HMS LINCS (Harvard Medical School)The KiNativ assay platform is based on the use of biotinylated acyl phosphates of ATP or ADP that act as probes by reacting with protein kinases on conserved lysine residues in the ATP binding pocket to covalently attach a biotin moiety. Using these probes, cell lysates treated with a kinase inhibitor can be labeled with BHAcATP or BHAcADP, with kinases inhibited by the compound of interest exhibiting reduced or no labeling. This is followed by digestion with trypsin, isolation of biotinylated peptides, and analysis by mass spectrometry to determine the extent of labeling of peptides from each kinase.LINCSfalseXMD8-92 KiNativ -- single dose experiment-2015-10-082015-10-08LDS-1088