{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000078450/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=2d15d0b456b44dc09036db4ffba10d39"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["201"],"data_protocol":[""],"pubmed_abstract":["The Hippo pathway regulates organ size and tissue homeostasis in response to multiple stimuli, including cell density and mechanotransduction. Pharmacological inhibition of phosphatases can also stimulate Hippo signaling in cell culture. We defined the Hippo protein-protein interaction network with and without inhibition of serine and threonine phosphatases by okadaic acid. We identified 749 protein interactions, including 599 previously unrecognized interactions, and demonstrated that several interactions with serine and threonine phosphatases were phosphorylation-dependent. Mutation of the T-loop of MST2 (mammalian STE20-like protein kinase 2), which prevented autophosphorylation, disrupted its association with STRIPAK (striatin-interacting phosphatase and kinase complex). Deletion of the amino-terminal forkhead-associated domain of SLMAP (sarcolemmal membrane-associated protein), a component of the STRIPAK complex, prevented its association with MST1 and MST2. Phosphatase inhibition produced temporally distinct changes in proteins that interacted with MOB1A and MOB1B (Mps one binder kinase activator-like 1A and 1B) and promoted interactions with upstream Hippo pathway proteins, such as MST1 and MST2, and with the trimeric protein phosphatase 6 complex (PP6). Mutation of three basic amino acids that are part of a phospho-serine- and phospho-threonine-binding domain in human MOB1B prevented its interaction with MST1 and PP6 in cells treated with okadaic acid. Collectively, our results indicated that changes in phosphorylation orchestrate interactions between kinases and phosphatases in Hippo signaling, providing a putative mechanism for pathway regulation.","<h4>Objective</h4>The primary objective of this study was to examine the patient comprehension of diabetes self-management instructions provided at hospital discharge as an associated risk of readmission.<h4>Methods</h4>Noncritically ill patients with diabetes completed patient comprehension questionnaires (PCQ) within 48 hours of discharge. PCQ scores were compared among patients with and without readmission or emergency department (ED) visits at 30 and 90 days. Glycemic measures 48 hours preceding discharge were investigated. Diabetes Early Readmission Risk Indicators (DERRIs) were calculated for each patient.<h4>Results</h4>Of 128 patients who completed the PCQ, scores were similar among those with 30-day (n = 31) and 90-day (n = 54) readmission compared with no readmission (n = 72) (79.9 ± 14.4 vs 80.4 ± 15.6 vs 82.3 ± 16.4, respectively) or ED visits. Clarification of discharge information was provided for 47 patients. PCQ scores of 100% were achieved in 14% of those with and 86% without readmission at 30 days (P = .108). Of predischarge glycemic measures, glycemic variability was negatively associated with PCQ scores (P = .035). DERRIs were significantly higher among patients readmitted at 90 days but not 30 days.<h4>Conclusion</h4>These results demonstrate similar PCQ scores between patients with and those without readmission or ED visits despite the need for corrective information in many patients. Measures of glycemic variability were associated with PCQ scores but not readmission risk. This study validates DERRI as a predictor for readmission at 90 days."],"pubmed_title":["Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions.","Patient Understanding of Discharge Instructions for Home Diabetes Self-Management and Risk for Hospital Readmission and Emergency Department Visits."],"pubmed_authors":["Couzens Amber L AL, Knight James D R JD, Kean Michelle J MJ, Teo Guoci G, Weiss Alexander A, Dunham Wade H WH, Lin Zhen-Yuan ZY, Bagshaw Richard D RD, Sicheri Frank F, Pawson Tony T, Wrana Jeffrey L JL, Choi Hyungwon H, Gingras Anne-Claude AC","Pinkhasova Diana D, Swami Janya B JB, Patel Neeti N, Karslioglu-French Esra E, Hlasnik Deborah S DS, Delisi Kristin J KJ, Donihi Amy C AC, Rubin Daniel J DJ, Siminerio Linda S LS, Wang Li L, Korytkowski Mary T MT"],"citation_count":["0"],"additional_accession":[]},"is_claimable":true,"name":"Couzens_Hippo_P86_VS4","description":"","dates":{"publication":"Fri Jun 28 00:00:00 BST 2013"},"accession":"MSV000078450","cross_references":{"pubmed":["24255178"]}}