{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000078764/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Riitta Lahesmaa"],"instrument_platform":["LTQ Orbitrap Velos"],"species":["Homo Sapiens (ncbitaxon:9606)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=fb659640191246cbad3da87f1ae21327"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["56"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["We determined longitudinal serum proteomics profiles from children with HLA-conferred diabetes susceptibility to identify changes that could be detected before seroconversion and positivity for disease-associated autoantibodies. Comparisons were made between children who seroconverted and progressed to type 1 diabetes (progressors) and those who remained autoantibody negative, matched by age, sex, sample periodicity, and risk group. The samples represented the prediabetic period and ranged from the age of 3 months to 12 years. After immunoaffinity depletion of the most abundant serum proteins, isobaric tags for relative and absolute quantification were used for sample labeling. Quantitative proteomic profiles were then measured for 13 case-control pairs by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, a label-free LC-MS/MS approach was used to analyze depleted sera from six case-control pairs. Importantly, differences in abundance of a set of proteins were consistently detected before the appearance of autoantibodies in the progressors. Based on top-scoring pairs analysis, classification of such progressors was observed with a high success rate. Overall, the data provide a reference of temporal changes in the serum proteome in healthy children and children progressing to type 1 diabetes, including new protein candidates, the levels of which change before clinical diagnosis."],"pubmed_title":["Serum proteomes distinguish children developing type 1 diabetes in a cohort with HLA-conferred susceptibility."],"pubmed_authors":["Moulder Robert R, Bhosale Santosh D SD, Erkkilä Timo T, Laajala Essi E, Salmi Jussi J, Nguyen Elizabeth V EV, Kallionpää Henna H, Mykkänen Juha J, Vähä-Mäkilä Mari M, Hyöty Heikki H, Veijola Riitta R, Ilonen Jorma J, Simell Tuula T, Toppari Jorma J, Knip Mikael M, Goodlett David R DR, Lähdesmäki Harri H, Simell Olli O, Lahesmaa Riitta R"],"citation_count":["0"],"additional_accession":[]},"is_claimable":true,"name":"D18_LF","description":"Thermo RAW files Case18T and Control18T (Case and Cont) represent D18 and Control-18 and are marked by their visit number. These are label free analyses of depleted serum samples conducted with an Orbitrap Velos - Proxeon capillary LC combination. CID fragmentation was used. The samples were analyzed in quadruplicate with randomization. Fifty-six  files in total from 14 serum samples.","dates":{"publication":"Wed Jun 25 04:35:00 BST 2014"},"accession":"MSV000078764","cross_references":{"pubmed":["25616278"]}}