{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000078980/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Steven A. Carr"],"instrument_platform":["Q Exactive"],"species":["Mus Musculus (ncbitaxon:10090)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=86ab0b7a619c42c9b8b6dd03baa812e4"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["215"],"ptm_modification":["UNIMOD:4 - \"Iodoacetamide derivative.\"","SILAC-R-0,6,10-K-0,4,8"],"data_protocol":[""],"pubmed_abstract":["Cell-type specific gene silencing by histone H3 lysine 27 and lysine 9 methyltransferase complexes PRC2 and G9A-GLP is crucial both during development and to maintain cell identity. Although studying their interaction partners has yielded valuable insight into their functions, how these factors are regulated on a network level remains incompletely understood. Here, we present a new approach that combines quantitative interaction proteomics with global chromatin profiling to functionally characterize repressive chromatin modifying protein complexes in embryonic stem cells. We define binding stoichiometries of 9 new and 12 known interaction partners of PRC2 and 10 known and 29 new interaction partners of G9A-GLP, respectively. We demonstrate that PRC2 and G9A-GLP interact physically and share several interaction partners, including the zinc finger proteins ZNF518A and ZNF518B. Using global chromatin profiling by targeted mass spectrometry, we discover that even sub-stoichiometric binding partners such as ZNF518B can positively regulate global H3K9me2 levels. Biochemical analysis reveals that ZNF518B directly interacts with EZH2 and G9A. Our systematic analysis suggests that ZNF518B may mediate the structural association between PRC2 and G9A-GLP histone methyltransferases and additionally regulates the activity of G9A-GLP."],"pubmed_title":["Functional Proteomic Analysis of Repressive Histone Methyltransferase Complexes Reveals ZNF518B as a G9A Regulator."],"pubmed_authors":["Maier Verena K VK, Feeney Caitlin M CM, Taylor Jordan E JE, Creech Amanda L AL, Qiao Jana W JW, Szanto Attila A, Das Partha P PP, Chevrier Nicholas N, Cifuentes-Rojas Catherine C, Orkin Stuart H SH, Carr Steven A SA, Jaffe Jacob D JD, Mertins Philipp P, Lee Jeannie T JT"],"pubmed_title_synonyms":["6820424L24Rik, Znf518b, Methyltransferase, AI661722, G9a, regulator., AA410078, Ng36, Histone H1(s), Histone, GAT8, KMT1C, Histone H2b, Histone H2a, G9A, proteomic analysis, Histone H5, D17Ertd710e, Histone H4, Histone H7, C6orf30, NG36, Histone H1, Bat8, Histone H3.3, BAT8, Histone H3"],"pubmed_abstract_synonyms":["MGC130048, Activity, Lysine Hydrochloride, GLP, Eu-HMTase1, Glp, determination, postnatal development, Stem Cells, ezh2, ezh1, A4, H3 Methyltransferase, Euchromatic histone-lysine N-methyltransferase 1, EZH2b, growth and development, protein, sci, 2-pyrrolidone-5-carboxylic acid, SUB, dmTAF[[II]]230, Histone H2b, Histone H2a, protein polypeptide chains, Lysine Acetate, DmelCG12298, Associations, Histone-Arginine, FP13812, D17Ertd710e, HOW, How, 10.5, 2, SCRAMBLED, Analysis, protein aggregate, KIF20A, l(3)j5D5, Chromatins, 5-Pyrrolidone-2-carboxylic acid, 10.9, L Lysine, Histone-Arginine N-Methyltransferase, 24B, Mass Spectrum Analysis, gamma sarcoglycan, F, mKIAA0335, Histone Arginine N Methyltransferase, TFIID TAF250, Analyses, DmelCG2328, cel, K, stru, epsilon-diaminocaproic acid, inhibition of gene expression, Fingers, proteins, V, l(3)S053606, CG10293, l(3)j5B5, LYS, Lysin, chromosome scaffold, Histone H5, gamma-sarcoglycan, Histone H4, Histone H7, Histone Methyltransferase, Histone H1, CLLL7, Histone H3, EZH1, bA188C12.1, nuclear chromatin, 0904/17, dTAF[[II]]230, SG-gamma, STRUBBELIG, TAF200, N-Methyltransferase, AI426493, TAFII-250, TAF250/230, G9a-like protein 1, Spectrum Analysis, Zfp518, GAT8, KMT1D, KMT1C, Spectroscopy, TAFII250, gene silencing, Stem Cell, SZ1, lysine, sarcoglycan, VI, Enx-1, C6orf30, 20.35, Methylase, AI661722, RP11-185C18.1, D330003E03, Spectrometry, Histone Methylase, Lysine N-methyltransferase 1D, alpha, AA410078, CG17603, gamma (35kDa dystrophin-associated glycoprotein), TAF[[II]], DMDA, EUHMTASE1, Finger, Taf250, SR3-5, 35kD dystrophin-associated glycoprotein, Cells, eve2, anon-EST:Liang-2.39, Embryonic Stem, Bat8, TAF230, BAT8, CG2328, mKIAA4065, 14.10, AT1G11140, 6820424L24Rik, cytoplasmic chromatin, Znf518b, d230, epigenetic, SGCG_HUMAN, Peptidomics, Znf518a, P62, enx1, enx-1, number, Gene, dTAFII250, kmt6, Spectrum Analyses, protein-containing complex, EfW1, presence, TYPE, CG12298, DAGA4, Zinc Finger Motifs, RGD1305314, polypeptide chain, dmTAF1, Taf230, xez, 35DAG, Gene Products, Mass, Lysine, even, MAM, gamma-SG, KMT6A, SCG3, Histone H3.3, Mass Spectroscopy, TAF250, ENX-1, Taf200, dTAF[[II]]250, Methyltransferase, l(3)s2612, cell, Embryonic Stem Cell, ligand, Zinc Finger Motif, mei-1794, Gene Inactivation, Taf1p, WVS, Pyroglutamic acid, dTAF250, ENX1, CLLD7, Enx1h, KMT6, Pyroglutamate, Zinc, DmelCG10293, Zinc Finger DNA Binding Domains, Motifs, SRF9, 5-oxo-DL-proline, WVS2, TAF, H3-K9-HMTase 5, TAF[[II]]250, 2.1.1.-, 35 kDa dystrophin-associated glycoprotein, Inactivation, clone 2.39, protein complex, Proteins, bA188C12.1., l(3)84Ab, BG:DS00004.13, qkr, l(3)S090417, Znf518, Histone, Cell, SGCG, LGMD2C, dTAF230, STRUBBELIG-RECEPTOR FAMILY 9, development, 2.1.1.43, count in organism, MS, GLP1, native protein, natural protein, Zinc Finger DNA-Binding Domains, Acetate, p230, KH93F, G9A, Protein, chemical analysis, Histone H3-K9 methyltransferase 5, TAF[[II]]250/230, TFIID, ZNF518, Eve, EVE, L-Lysine, Histone H3 Methyltransferase, Silencing, Mass Spectrum Analyses, who, Embryonic, Taf[[II]]250, Mass Spectrum, TAF[[II]]230, DMDA1, G9a, Dub, postnatal growth, l(2)46Ce, TAF[II]250, Who/How, Ng36, Histone H1(s), Zinc Finger, 2810401C22Rik, l(2)46Cg, 9230102N17Rik, Protein Gene Products, l(2)46CFj, Gene Proteins, 6330417C12Rik, l(2)46CFh, DmelCG17603, mKIAA1876, l(2)46CFp, SCARMD2, qkr[93F], PRC2, Methyltransferases, assay, NG36, 6-diaminohexanoic acid, E(eve), growth, T19D16.8, Enisyl, General activity, SCM, l(2)46CFg, Motif, TAF1"],"name_synonyms":["KMT1C, 6820424L24Rik, Znf518b, AI661722, Peptidomics, G9A, G9a, D17Ertd710e, regulator., PRC2, C6orf30, NG36, Ng36, AA410078, Bat8, BAT8, GAT8"],"citation_count":["0"],"additional_accession":[]},"is_claimable":true,"name":"Functional proteomics defines a PRC2-G9A interaction network and reveals ZNF518B as a G9A regulator","description":"","dates":{"publication":"Wed Dec 17 18:52:00 GMT 2014"},"accession":"MSV000078980","cross_references":{"pubmed":["25680957"]}}