{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000079073/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Bradford W. Gibson, Alan J. Wolfe"],"instrument_platform":["TripleTOF 6600"],"species":["Escherichia Coli (ncbitaxon:562)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=a951c6594e604490ba53d393029271a8"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["708"],"ptm_modification":["MOD:00064 - \"A protein modification that effectively converts an L-lysine residue to N6-acetyl-L-lysine.\""],"data_protocol":[""],"pubmed_abstract":["In Escherichia coli, acetylation of proteins at lysines depends largely on a non-enzymatic acetyl phosphate-dependent mechanism. To assess the functional significance of this post-translational modification, we first grew wild-type cells in buffered tryptone broth with glucose and monitored acetylation over time by immunochemistry. Most acetylation occurred in stationary phase and paralleled glucose consumption and acetate excretion, which began upon entry into stationary phase. Transcription of rprA, a stationary phase regulator, exhibited similar behavior. To identify sites and substrates with significant acetylation changes, we used label-free, quantitative proteomics to monitor changes in protein acetylation. During growth, both the number of identified sites and the extent of acetylation increased with considerable variation among lysines from the same protein. As glucose-regulated lysine acetylation was predominant in central metabolic pathways and overlapped with acetyl phosphate-regulated acetylation sites, we deleted the major carbon regulator CRP and observed a dramatic loss of acetylation that could be restored by deleting the enzyme that degrades acetyl phosphate. We propose that acetyl phosphate-dependent acetylation is a response to carbon flux that could regulate central metabolism."],"pubmed_title":["Protein acetylation dynamics in response to carbon overflow in Escherichia coli."],"pubmed_authors":["Schilling Birgit B, Christensen David D, Davis Robert R, Sahu Alexandria K AK, Hu Linda I LI, Walker-Peddakotla Arti A, Sorensen Dylan J DJ, Zemaitaitis Bozena B, Gibson Bradford W BW, Wolfe Alan J AJ"],"pubmed_abstract_synonyms":["extent, biochemical pathways, Post Translational Amino Acid Modification, MGC130048, Metabolic Process, posttranslational modification, AP-4, Esters, Lysine Hydrochloride, Glukose, postnatal development, ethanoate, Metabolic Concepts, A4, bacterium E3, Monohydrate, growth and development, CG7664, protein, (DL)-Isomer, Anabolism., Protein Processing, Long Term, protein polypeptide chains, Lysine Acetate, Carbon-12, Dextrose, Concepts, 2, excretion, gp300, Enterococcus coli, Metabolism Concept, protein aggregate, Phenomenon, Effect, L Lysine, Post-Translational Protein, gamma sarcoglycan, increased, C, posttranslational amino acid modification, Escherichia/Shigella coli, Post-Translational, K, D1S181E, catabolism, Acetic Acid, epsilon-diaminocaproic acid, HEL-141, E coli, AW743261, proteins, metabolic process resulting in cell growth, Posttranslational Protein Processing, free, DL-glucose, E. coli, CH3-COO(-), Eschericia coli, Enteroaggregative Escherichia coli, glucose, LYS, Lysin, Carbon, gamma-sarcoglycan, biotransformation, transcription from bacterial-type RNA polymerase promoter, Catabolism, Long-Term Effects, single-organism behavior, Post-Translational Modification, Epididymis luminal protein 141, Process, completeness, CRP4, CRP2, ACETATE ION, CRP1, metabolism resulting in cell growth, acetylation, Modifications, SG-gamma, Longterm Effect, Acetic Acid Esters, Enteroaggregative E. coli, Acceptance Processes, Vitreous, Acceptance Process, DmelCG7664, Post Translational Modifications, Diffusely Adherent E. coli, lysine, CSRP, sarcoglycan, crp.4a, secretion, (alpha-D)-Isomer, regulator, Ethanoat, D-Glucose, Diffusely Adherent Escherichia coli, Ab1-341, PTM, Enteroinvasive E. coli, l(2)00232, growth pattern, non-developmental growth, 6C, Processing, alpha, gamma (35kDa dystrophin-associated glycoprotein), loss of, Crpd, Vitreous Carbon, DMDA, Amino Acid Modification, D Glucose, post-translational modification, 35kD dystrophin-associated glycoprotein, label, bacterial transcription, carbonium, Biocatalyst, Posttranslational Modifications, Acids, Glucose Monohydrate, Posttranslational Modification, accessory, Aa1249, gluco-hexose, Ba2-693, Cysteine-rich protein 1, CRP-[a], Post-Translational Protein Modifications, SGCG_HUMAN, Glucose, Effects, Peptidomics, Processes, Biocatalysts, 35F(AT)[[17]], number, Gene, carbon, l(2)35Fd, Metabolic Processes, protein-containing complex, presence, supernumerary, TYPE, excreted substance, Posttranslational, DAGA4, l(2)SH1614, polypeptide chain, Azetat, CYRP, Metabolism, Escherchia coli, 35DAG, ESP1, Gene Products, Post Translational Modification, l(2)k00809, Post-Translational Modifications, Enteroinvasive Escherichia coli, Lysine, MAM, gamma-SG, SCG3, Metabolism Phenomena, Post Translational, Modification, Longterm, protein amino acid acetylation, Acetylations, Metabolic Concept, posttranslational protein modification, Long-Term, Ac1-114, Protein Modifications, Post-Translational Amino Acid Modification, Kohlenstoff, l(2)k03505, Enzyme, CRP-[b], p80, Posttranslational Amino Acid Modification, Acetic Acids, 0610010I23Rik, Posttranslational Protein, Long-Term Effect, Behaviors, AI255847, Anhydrous, BG:DS02740.3, Alkalescens-Dispar Group, Bacterium coli, (beta-D)-Isomer, waste substance, ptx1, 35 kDa dystrophin-associated glycoprotein, degradation, protein complex, Proteins, cell cycle quiescence, Post-Translational Protein Modification, ion(1-), portion of excreted substance, EAggEC, carbone, Cell, SGCG, LGMD2C, Concept, Metabolic Phenomena, carbono, development, Metabolism Concepts, count in organism, native protein, natural protein, Ac1262, Bacillus coli, Acetate, Protein, Long Term Effects, Phenomena, Protein Modification, L-Lysine, metabolism, Metabolic Phenomenon, Crp, CRP, Acceptance, multicellular organism metabolic process, Acetic, DMDA1, biodegradation, Metabolic, MS:35F.AT17, Post Translational Protein Processing, increased number, Hlp, bHLHe63, postnatal growth, l(2)SH2 1614, Ab2-196, post-translational amino acid modification, Anhydrous Dextrose, Longterm Effects, Carbon 12, Protein Gene Products, Gene Proteins, present in greater numbers in organism, stationary phase, Post Translational Protein Modification, AP4, dAP-4, Bacterium coli commune, Ac2-069, SCARMD2, cardinality, Post-Translational Protein Processing, MeCO2 anion, DBMT1, acetic acid, PTX1, 6-diaminohexanoic acid, growth, Enisyl, C77570, Glc"],"pubmed_title_synonyms":["Alkalescens-Dispar Group, C, Diffusely Adherent Escherichia coli, Bacterium coli, Escherichia/Shigella coli, Enteroinvasive E. coli, 6C, protein amino acid acetylation, bacterium E3, E coli, carbon, EAggEC, carbone, Enteroaggregative E. coli, E. coli., Carbon 12, carbono, Eschericia coli, Enteroaggregative Escherichia coli, Vitreous, Kohlenstoff, Vitreous Carbon, Bacterium coli commune, Carbon-12, Diffusely Adherent E. coli, Bacillus coli, Carbon, Escherchia coli, carbonium, Enteroinvasive Escherichia coli, Enterococcus coli"],"name_synonyms":["carbono, Vitreous, C, Kohlenstoff, Vitreous Carbon, Bacterium coli, Escherichia/Shigella coli, Bacterium coli commune, Carbon-12, Bacillus coli, Carbon 12., Carbon, 6C, protein amino acid acetylation, carbonium, Enterococcus coli, carbon, carbone, E. coli"],"citation_count":["0"],"additional_accession":["PXD001894"]},"is_claimable":true,"name":"Dynamic protein acetylation changes in E. coli (carbon overflow)","description":"","dates":{"publication":"Mon Mar 09 16:06:00 GMT 2015"},"accession":"MSV000079073","cross_references":{"pubmed":["26264774"]}}