{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000079097/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":{"citationCount":1,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Steven A. Carr"],"instrument_platform":["Q Exactive"],"species":["Homo Sapiens (ncbitaxon:9606)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=1ebc34f0b2dc4fb3a4b3d1e1983ed00f"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["103"],"ptm_modification":["MOD:00492 - \"A protein modification that crosslinks the N6-amino of a peptidyl lysine with the carboxyl of glycylglycine, the two glycine residues left after tryptic digestion of ubiquitin.\"","SILAC-R-0,6,10-K-0,4,8","M-oxidation","C-carbamidomethyl"],"data_protocol":[""],"pubmed_abstract":["Detection of endogenous ubiquitination sites by mass spectrometry has dramatically improved with the commercialization of anti-di-glycine remnant (K-ε-GG) antibodies. Here, we describe a number of improvements to the K-ε-GG enrichment workflow, including optimized antibody and peptide input requirements, antibody cross-linking, and improved off-line fractionation prior to enrichment. This refined and practical workflow enables routine identification and quantification of ∼20,000 distinct endogenous ubiquitination sites in a single SILAC experiment using moderate amounts of protein input."],"pubmed_title":["Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments."],"pubmed_authors":["Udeshi Namrata D ND, Svinkina Tanya T, Mertins Philipp P, Kuhn Eric E, Mani D R DR, Qiao Jana W JW, Carr Steven A SA"],"name_synonyms":["opsonin activity, antibody, immunoglobulin, BCR complex, B cell receptor accessory molecule complex, membrane bound, B lymphocyte receptor complex, Ubiquitylation., B cell receptor activity, ubiquitination, B-cell receptor complex, B-lymphocyte receptor complex, antibodies, immunoglobulin complex"],"pubmed_title_synonyms":["opsonin activity, Peptidomics., antibody, immunoglobulin, BCR complex, Ubiquitylation, B cell receptor accessory molecule complex, membrane bound, B lymphocyte receptor complex, B cell receptor activity, ubiquitination, B-cell receptor complex, B-lymphocyte receptor complex, antibodies, immunoglobulin complex"],"pubmed_abstract_synonyms":["Glycine Carbonate (2:1), Calcium Salt Glycine, Sodium Hydrogen Carbonate, H2N-CH2-COOH, Aminoessigsaeure, Monopotassium, number, Gly, B-cell receptor complex, protein, Spectrum Analyses, Monoammonium, protein-containing complex, Monosodium, peptide, antibody, Polypeptides, protein polypeptide chains, BCR complex, peptido, polypeptide chain, Calcium Salt (2:1), Mass, Monoammonium Salt Glycine, Gene Products, Analysis, Glycine Hydrochloride (2:1), Work Flow, protein aggregate, immunoglobulin complex, Mass Spectroscopy, Mass Spectrum Analysis, Ubiquitylation, G, Glycine Hydrochloride, peptides, Analyses, Glycine, membrane bound, Monosodium Salt, Phosphate, proteins, Salt Glycine, Glycin, Glycocoll, Leimzucker, peptidos, 2-aminoacetic acid, ubiquitination, B-lymphocyte receptor complex, Gene., Monoammonium Salt, Cobalt Salt Glycine, Glycine Phosphate (1:1), Glycine Phosphate, protein complex, Monopotassium Salt Glycine, Proteins, Aminoacetic acid, Copper Salt, Monosodium Salt Glycine, aminoethanoic acid, antibodies, Spectrum Analysis, Peptide, Copper Salt Glycine, Spectroscopy, polypeptide, Workflows, MS, Glycine Carbonate (1:1), Experiment, native protein, natural protein, Protein, Hydrochloride, Mass Spectrum Analyses, opsonin activity, Mass Spectrum, Acid, Aminoacetic Acid, distinct, Glycine Sulfate (3:1), Hgly, Spectrometry, Monopotassium Salt, Cobalt Salt, Monolithium Salt, Glyzin, Protein Gene Products, requirements, Gene Proteins, immunoglobulin, B cell receptor accessory molecule complex, Aminoacetic, Glykokoll, cardinality, B lymphocyte receptor complex, B cell receptor activity, Peptid, Polypeptide, Work Flows, Calcium Salt"],"citation_count":["1"],"additional_accession":["PXD002018"]},"is_claimable":true,"name":"Refined preparation and use of K-GG antibody enables routine quantification of ubiquitination sites","description":"","dates":{"publication":"Fri Apr 03 12:03:00 BST 2015"},"accession":"MSV000079097","cross_references":{"pubmed":["23266961"]}}