MassIVEapplication/xmlftp://massive.ucsd.edu/v01/MSV000079300/primaryOK2000000ProteomicsDr Jean ArmengaudLTQ OrbitrapModestobacter Marinus Bc501 (ncbitaxon:1144889)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=5bb0c0bc12ac46f987044eec9eca7a1cMassIVE10UNIMOD:4 - "Iodoacetamide derivative."UNIMOD:35 - "Oxidation or Hydroxylation."The Geodermatophilaceae are unique model systems to study the ability to thrive on or within stones and their proteogenomes (referring to the whole protein arsenal encoded by the genome) could provide important insight into their adaptation mechanisms. Here we report the detailed comparative genome analysis of Blastococcus saxobsidens (Bs), Modestobacter marinus (Mm) and Geodermatophilus obscurus (Go) isolated respectively from the interior and the surface of calcarenite stones and from desert sandy soils. The genome-scale analysis of Bs, Mm and Go illustrates how adaptation to these niches can be achieved through various strategies including 'molecular tinkering/opportunism' as shown by the high proportion of lost, duplicated or horizontally transferred genes and ORFans. Using high-throughput discovery proteomics, the three proteomes under unstressed conditions were analyzed, highlighting the most abundant biomarkers and the main protein factors. Proteomic data corroborated previously demonstrated stone-related ecological distribution. For instance, these data showed starvation-inducible, biofilm-related and DNA-protection proteins as signatures of the microbes associated with the interior, surface and outside of stones, respectively.Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes.Sghaier Haïtham H, Hezbri Karima K, Ghodhbane-Gtari Faten F, Pujic Petar P, Sen Arnab A, Daffonchio Daniele D, Boudabous Abdellatif A, Tisa Louis S LS, Klenk Hans-Peter HP, Armengaud Jean J, Normand Philippe P, Gtari Maher Mscale tissue, Biological Markers, Viral Marker, Materials, Surrogate Endpoints, DNS, determination, (Deoxyribonucleotide)n, Peptidomics, Clinical Markers, Laboratory, P62, Clinical Marker, peltate hair, Biochemical, Gene, Endpoint, protein, sci, Yperite, Serum, protein-containing complex, Deoxyribonucleic acids, Surrogate End Points, Surrogate Markers, protein polypeptide chains, surface, Laboratory Markers, polypeptide chain, Deoxyribonucleic Acid, Biological, Gene Products, HOW, How, 1, Mustard gas, protein aggregate, 1'-thiobis(2-chloroethane), l(3)j5D5, integumentum commune, 24B, study, Biomarker, proportion, doubled, thymus nucleic acid, l(3)s2612, Genetic, Clinical, pattern, Genomes, distribution, plant peltate hair, Iprit, Biological Marker, anon-WO0118547.126, stru, Double Stranded, proteins, Deoxyribonucleic acid, l(3)S053606, growl, CG10293, bis(2-chloroethyl) sulphide, Lost, st, l(3)j5B5, Immunologic Markers, Immune, CG14648, Markers, 1-chloro-2-[(2-chloroethyl)sulfanyl]ethane, Viral Markers, mustard gas, DmelCG10293, scale (sensu Metazoa), DmelCG14648, Double-Stranded DNA, (Deoxyribonucleotide)m, deoxyribonucleic acids, DNAn, associated, Immunologic Marker, Biologic, sulfur mustard, ratio, 0904/17, data, Growl, Viral, external covering of organism, Surrogate Endpoint, 1-chloro-2-[(2-chloroethyl)thio]ethane, protein complex, clone 2.39, DNAn+1, Serum Markers, Proteins, End Point, organism surface, Biochemical Markers, Double-Stranded, qkr, bis(2-chloroethyl) sulfide, l(3)S090417, Cistrons, Biologic Marker, nev, Immune Marker, (Deoxyribonucleotide)n+m, bis(2-chloroethyl)sulfane, native protein, natural protein, SZ1, Marker, Surrogate End Point, KH93F, Protein, chemical analysis, Genetic Materials, scales, ds-DNA, Biofilm, desoxyribose nucleic acid, Genetic Material, who, Biologic Markers, Serum Marker, integumentum commune., scale, End Points, body surface, Surrogate, Endpoints, proportionality, Senfgas, rate, Who/How, Immunologic, Laboratory Marker, Surrogate Marker, Protein Gene Products, Gene Proteins, integumentary system, Material, Biochemical Marker, ds DNA, quotient, Desoxyribonukleinsaeure, qkr[93F], anon-EST:Liang-2.39, Cistron, assay, DNA, dermal system, Proteomes, Immune Markersbacteria, Eubacteria, Bacteria <bacteria>, Prokaryotae, prokaryote, Proteomes., prokaryotes, total expressed protein, fungi, Procaryotae, eubacteria, Monera, Prokaryotaactinobacteria, st, Actinomycete, Actinobacteraeota, High G+C Gram-Positive Bacteria., Actinobacteria, Actinomycetes, Actinobacteriota, Gram-Positive Bacteria, High G+Cactinobacteria, st, Actinomycete, Actinobacteraeota, High G+C Gram-Positive Bacteria., Actinobacteria, Actinomycetes, Actinobacteriota, Gram-Positive Bacteria, High G+C0trueStone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomesThis project contributes to the proteomic comparison of different bacteria. A whole proteome catalogue was obtained for Modestobacter marinus grown in standard conditions.MSV00007930026125681