{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000079369/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Ruibing Chen"],"instrument_platform":["LTQ Orbitrap Discovery"],"species":["Homo Sapiens"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=af2dda85e6e5497a974444fc7ffcf1fe"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["64"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["The study of protein-protein interactions is an essential process to understand the biological functions of proteins and the underlying mechanisms. Co-immunoprecipitation coupled with mass spectrometry (CoIP-MS) is one of the most extensively used high-throughput techniques to discover novel protein-protein interactions. However, the traditional CoIP process uses whole cell lysate, disrupts cellular organization, and leads to potential false positives by inducing artificial protein-protein interactions. Here, we have developed a strategy by combining subcellular fractionation with CoIP-MS to study the interacting proteins of the complement component 1, q subcomponent binding protein (C1QBP) in the mitochondria. Using this method, a novel C1QBP interacting protein, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT) was identified and validated. Furthermore, the activity of the pyruvate dehydrogenase (PDH) was found to be affected by the expression level of C1QBP. These results provide novel insights regarding the mitochondrial function of C1QBP in the regulation of cellular energy metabolism. This method could also be used to analyze the subcellular protein-protein interactions for other proteins of interest."],"pubmed_title":["Identification of a novel mitochondrial interacting protein of C1QBP using subcellular fractionation coupled with CoIP-MS."],"pubmed_authors":["Chen Ruibing R, Xiao Mingming M, Gao Huajun H, Chen Yajing Y, Li Yongmei Y, Liu Yunde Y, Zhang Ning N"],"pubmed_title_synonyms":["Mitochondrial matrix protein p32, AA986492, Mitochondrial, SF2P32, GC1QBP, gC1Q-R, protein complex, gC1q-R protein, p32, Habp1, Proteins, p33, Gene, proteins, protein, D11Wsu182e, protein-containing complex, ASF|SF2-associated protein p32, mitochondrial, Protein Gene Products, gC1qBP, Gene Proteins, protein polypeptide chains, SF2p32, native protein, ATP synthase D chain, natural protein, polypeptide chain, AA407365, Glycoprotein gC1qBP, Protein, P32, Gene Products, GC1q-R protein, HABP1., protein aggregate, C1qBP, Hyaluronan-binding protein 1, gC1qR"],"description_synonyms":["Co-Immunoprecipitations, AA986492, KIT binding, Dihydrolipoamide Acetyltransferase, artificial sequence, Clo, Procedures, False, Slf, SLF, Transacetylase, pyruvate dehydrogenase complex (lipoamide), Gene, organization and administration, Non-Governmental Organizations, FPH2, protein, Spectrum Analyses, protein-containing complex, Mitochondrial Contractions, mitochondrial, Dihydrolipoyl, contrasted, HABP1, protrusion, Co Immunoprecipitation, dihydrolipoyl dehydrogenase complex, Protein Gm1141, protein polypeptide chains, method, Techniques, polypeptide chain, PDC-E2, Method, AA407365, method used in an experiment, Gene Products, Immune Precipitations, Mass, Mitochondrion, Studies, DCB-45, Non-Governmental, GC1q-R protein, Analysis, protein aggregate, Technique, Immune Precipitation, Mass Spectroscopy, Mass Spectrum Analysis, p32-RACK, study, Organization, Dehydrogenase Complex, F, q subcomponent binding protein, Acetyl CoA Dihydrolipoamide S Acetyltransferase, Analyses, S-Acetyltransferase, gC1Q-R, gC1q-R protein, Contraction, Co-Immunoprecipitation, Complex, Precipitation, Lipoate, SF, Kitl, Dihydrolipoamide S-Acetyltransferase, proteins, CG9148, Acetyltransferase, Pyruvate Dehydrogenase Complex E2, Mast cell growth factor, Study, Con, Immune, lysate, Non-Governmental Organization, Methodological Studies, blz, Dihydrolipoyllysine Residue Acetyltransferase, Dihydrolipoyl Transacetylase, 6332404G05Rik, PDCE2., Soluble KIT ligand, stem cell factor receptor ligand, Dihydrolipoyllysine-Residue, Precipitations, Organizations, organization, Sl, gC1qR, anon-EST:Posey14, Dihydrolipoamide S Acetyltransferase, SF2P32, GC1QBP, anatomical protrusion, organisation, organizational structure, protein complex, Proteins, Novel protein, artificial gene, synthetic DNA, Procedure, ASF|SF2-associated protein p32, Spectrum Analysis, isoform CRA_b, sKITLG, Spectroscopy, Acetyl-CoA-Dihydrolipoamide S-Acetyltransferase, Contractions, SF2p32, MS, SCFR binding, native protein, natural protein, ATP synthase D chain, Protein, P32, synthetic, Nongovernmental Organizations, C1qBP, Mass Spectrum Analyses, Acetyl-CoA-Dihydrolipoamide, administrative management, Mass Spectrum, BEST:CK02682, Mitochondrial matrix protein p32, SHEP7, Mitochondrial, Dihydrolipoyl Acetyltransferase, mast cell growth factor, Nongovernmental, p32, Habp1, administrative structure, Stem cell factor, p33, Spectrometry, DLTA, D11Wsu182e, Methodological, Pyruvate Dehydrogenase, synthetic constructs, Methodological Study, Lipoate Acetyltransferase, Protein Gene Products, Mitochondrial Contraction, plan specification, gC1qBP, Gene Proteins, stem cell factor, Kitlg, SYNTHETIC CONSTRUCT sequences, DmelCG9148, Non Governmental Organizations, KL-1, mitochondria, Pyruvate, Complement component 1, spine, MGF, Mgf, Glycoprotein gC1qBP, c-Kit ligand, organizational management, artificial, Dihydrolipoamide, SCF, Nongovernmental Organization, Gb, Hyaluronan-binding protein 1, CK02682"],"name_synonyms":["gC1qBP, Mitochondrial matrix protein p32, AA986492, SF2P32, Mitochondrial, SF2p32, GC1QBP, ATP synthase D chain, gC1Q-R, AA407365, Glycoprotein gC1qBP, gC1q-R protein, p32, Habp1, P32, p33, GC1q-R protein, mitochondrial., D11Wsu182e, C1qBP, ASF|SF2-associated protein p32, Hyaluronan-binding protein 1, gC1qR, HABP1"],"pubmed_abstract_synonyms":["Oxidases, Co-Immunoprecipitations, Dehydrogenases, artificial sequence, Product, Activity, Metabolisms, Non-Governmental Organizations, Bioenergetics, protein, Mitochondrial Contractions, Social Controls, HABP1, Co Immunoprecipitation, protein polypeptide chains, Techniques, PDC-E2, Biological, Method, GC1q-R protein, Analysis, Biological Product, Expenditure, protein aggregate, Formal Social Controls, Mass Spectrum Analysis, Reductases, Oxidoreductase, q subcomponent binding protein, Biologic Drugs, Analyses, Natural, gC1q-R protein, Co-Immunoprecipitation, Precipitation, proteins, pyranose:acceptor oxidoreductase activity, procedures, Biological Drugs, quinone-dependent pyranose dehydrogenase activity, Social, pyruvate:lipoamide 2-oxidoreductase (decarboxylating and acceptor-acetylating) activity, Biological Medicine, Immune, Methodological Studies, Medicine, Medicines, Precipitations, Organizations, 2-oxopropanoic acid, Biologic Drug, gC1qR, anatomical protrusion, organisation, Biologic Products, Pyruvic, deficiency of pyruvic dehydrogenase, Procedure, Spectrum Analysis, Spectroscopy, ATP synthase D chain, Biopharmaceuticals, P32, pyruvate dehydrogenase complex deficiency, Biologic Product, C1qBP, acceptor-acetylating), Mitochondrial matrix protein p32, Acid, Mitochondrial, Oxidase, Biological Medicines, Nongovernmental, Biological Drug, p32, administrative structure, p33, Spectrometry, Control, DLTA, PDH, Biologics, Methodological, D11Wsu182e, Controls, Methodological Study, gC1qBP, SYNTHETIC CONSTRUCT sequences, Energy Metabolisms, Pyruvate, spine, Glycoprotein gC1qBP, pyruvate dehydrogenase (lipoamide) activity, artificial, pdh, Regulation, Hyaluronan-binding protein 1, pyruvate dehydrogenase deficiency, Regulations, AA986492, Procedures, Biologic Pharmaceuticals, Dehydrogenase, Gene, organization and administration, Spectrum Analyses, protein-containing complex, PDCE2, mitochondrial, protrusion, Protein Gm1141, method, polypeptide chain, AA407365, Metabolism, method used in an experiment, Gene Products, Immune Precipitations, Mass, Studies, Mitochondrion, Non-Governmental, Technique, Immune Precipitation, Mass Spectroscopy, Biologicals, Drugs, p32-RACK, study, Organization, gC1Q-R, Contraction, Natural Product, Energy, pyruvate:dihydrolipoyllysine-residue acetyltransferase-lipoyllysine 2-oxidoreductase (decarboxylating, Expenditures, CG4899, Study, lysate, Non-Governmental Organization, pyruvate dehydrogenase complex deficiency disease, 6332404G05Rik, organization, Biologic, Pharmaceuticals, Products, MtPDC (mitochondrial pyruvate dehydogenase complex) activity, SF2P32, GC1QBP, organizational structure, 2-oxopropanoate, Formal Social Control, protein complex, Biologic Medicines, Proteins, Novel protein, ion(1-), artificial gene, pyranose-quinone oxidoreductase activity, function, synthetic DNA, ASF|SF2-associated protein p32, isoform CRA_b, Contractions, SF2p32, MS, native protein, pyruvate dehydrogenase complex activity, natural protein, Social Control, Protein, Biopharmaceutical, synthetic, techniques, ACE activity, Nongovernmental Organizations, Mass Spectrum Analyses, Reductase, Energy Expenditure, administrative management, Mass Spectrum, PDHC, Protein., Habp1, Energy Expenditures, synthetic constructs, Bioenergetic, Protein Gene Products, Drug, Mitochondrial Contraction, plan specification, Gene Proteins, Non Governmental Organizations, mitochondria, Pyruvate Dehydrogenase Complex Deficiency, Complement component 1, DmelCG4899, Natural Products, organizational management, pyranose dehydrogenase activity, regulation, pcdr, Nongovernmental Organization, General activity, pyruvate decarboxylase deficiency, methodology"],"citation_count":["0"],"additional_accession":["PXD003148"]},"is_claimable":false,"name":"C1QBP mitochondrial interactome","description":"The study of protein-protein interactions is an essential process to understand the biological functions of proteins and the underlying mechanisms. Co-immunoprecipitation coupled with mass spectrometry (CoIP-MS) is one of the most extensively used high-throughput techniques to discover novel protein-protein interactions. However, the traditional CoIP process uses whole cell lysate, disrupts cellular organization, and leads to potential false positives by inducing artificial protein-protein interactions. Here we have developed a strategy by combining subcellular fractionation (SCF) with CoIP -MS to study the interacting proteins of the complement component 1, q subcomponent binding protein (C1QBP) in the mitochondria. Using this method, a novel C1QBP interacting protein, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT) was identified and validated.  ","dates":{"publication":"Wed Nov 04 21:02:00 GMT 2015"},"accession":"MSV000079369","cross_references":{"pubmed":["26753982"],"TAXONOMY":["9606"]}}