{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000080631/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Anne-Claude Gingras"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=89c9b4db3ec24d2f8c572235782dc0e0"],"submitter_email":["gingras@lunenfeld.ca"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["54"],"ptm_modification":["MOD:00685 - \"A protein modification that effectively converts an L-glutamine residue to L-glutamic acid.\"","MOD:00719 - \"A protein modification that oxygenates an L-methionine residue to one of the diastereomeric L-methionine sulfoxide residues.\"","MOD:00684 - \"A protein modification that effectively converts an L-asparagine residue to L-aspartic acid.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["LTQ Orbitrap Velos","LTQ Orbitrap Elite"],"species":["Homo Sapiens (ncbitaxon:9606)"],"submitter_affiliation":["LTRI"],"pubmed_abstract":["<h4>Background</h4>In addition to DNA, gametes contribute epigenetic information in the form of histones and non-coding RNA. Epigenetic programs often respond to stressful environmental conditions and provide a heritable history of ancestral stress that allows for adaptation and propagation of the species. In the nematode C. elegans, defective epigenetic transmission often manifests as progressive germline mortality. We previously isolated sup-46 in a screen for suppressors of the hexosamine pathway gene mutant, gna-2(qa705). In this study, we examine the role of SUP-46 in stress resistance and progressive germline mortality.<h4>Results</h4>We identified SUP-46 as an HNRNPM family RNA-binding protein, and uncovered a highly novel role for SUP-46 in preventing paternally-mediated progressive germline mortality following mating. Proximity biotinylation profiling of human homologs (HNRNPM, MYEF2) identified proteins of ribonucleoprotein complexes previously shown to contain non-coding RNA. Like HNRNPM and MYEF2, SUP-46 was associated with multiple RNA granules, including stress granules, and also formed granules on active chromatin. SUP-46 depletion disrupted germ RNA granules and caused ectopic sperm, increased sperm transcripts, and chronic heat stress sensitivity. SUP-46 was also required for resistance to acute heat stress, and a conserved \"MYEF2\" motif was identified that was needed for stress resistance.<h4>Conclusions</h4>In mammals, non-coding RNA from the sperm of stressed males has been shown to recapitulate paternal stress phenotypes in the offspring. Our results suggest that HNRNPM family proteins enable stress resistance and paternally-mediated epigenetic transmission that may be conserved across species."],"pubmed_title":["C. elegans SUP-46, an HNRNPM family RNA-binding protein that prevents paternally-mediated epigenetic sterility."],"pubmed_authors":["Johnston Wendy L WL, Krizus Aldis A, Ramani Arun K AK, Dunham Wade W, Youn Ji Young JY, Fraser Andrew G AG, Gingras Anne-Claude AC, Dennis James W JW"],"pubmed_title_synonyms":["Networks, Reproductive, RNA, Ribonucleic, HNRPM4, HTGR1, Family Member, Kinship, Family Research, ribose nucleic acid, CEAR, protein complex, FON1, Proteins, ribonucleic acids, HNRPM, Gene, SUPERMAN, RNS, Synaptosomal-associated 25 kDa protein, Network, protein, FLORAL ORGAN NUMBER 1, protein-containing complex, mKIAA4193, hnRNP M, Family Members, Hnrpm, Subfertility, protein polypeptide chains, native protein, natural protein, polypeptide chain, Reproductive Sterility., yeast nucleic acid, Ribonukleinsaeure, Protein, SUP, Gene Products, GENA70, Life Cycle, pentosenucleic acids, Ribonucleic acids, protein aggregate, Filiation, Non Polyadenylated, RNA Gene Products, Family Life Cycle, ribonucleic acid, Acid, Kinship Network, Research, Epigenetic, Non Polyadenylated RNA, ligand, 2610023M21Rik, Bdr, Non-Polyadenylated, FLO10, proteins, HNRNPM4, Ribonucleic Acid, Super protein, HERP, sp, Sub-Fertility, SNAP-25, Epigenomic, AA409009, Non-Polyadenylated RNA, Protein Gene Products, Life Cycles, Gene Proteins, Mif1, Hnrpm4, Families, SNAP, infertility, Sterility, Kinship Networks, Family Life Cycles, Epigenetics, Family, NAGR1, Relatives, FLORAL DEFECTIVE 10"],"description_synonyms":["293 cell, 293T Cell, HEK293 cell, 293 Cells, HNRPM4, HTGR1, CEAR, MEF-2, 2610023M21Rik, Human Embryonic Kidney Cell Line 293, HNRPM, mKIAA1341, HEK293, Human Kidney Cell Line 293, MSTP156, HEK293 Cell, HNRNPM4, mKIAA4193, HEK cell, HEK 293 Cell, Cell, hnRNP M, 293T, AA409009, A-293 cell, A293 cell, HEK 293 Cell Line, Hnrpm, HsT18564, HEK293., HEK 293 Cells, Hnrpm4, Cells, HEK, 293T Cells, human embryonic kidney cell, HEK 293, human embryonal kidney cell, MST156, NAGR1, myEF-2, 293 Cell, 9430071B01"],"pubmed_abstract_synonyms":["Forms, Networks, Ribonucleic, Materials, HTGR1, Kinship, CEAR, FON1, protein, FLORAL ORGAN NUMBER 1, Case Fatality Rates, Histone H2b, Death Rates, Histone H2a, protein polypeptide chains, ribonucleoprotein, Roles, Hot, SUP, Concepts, Life Cycle, Heat, Excess Mortalities, protein aggregate, Non Polyadenylated, Chromatins, RNA Gene Products, Family Life Cycle, X-Chromosome-Bearing Sperm, sperm cell, increased, thymus nucleic acid, X-Bearing, cell fusion, Kinship Network, Man (Taxonomy), Crude Mortality Rates, 2610023M21Rik, Bdr, proteins, HNRNPM4, Histories, Super protein, sp, Epigenomic, SNAP-25, Biotinylations, roundworm, Y Chromosome Bearing Sperm, Y-Bearing Sperm, X Chromosome Bearing Sperm, CFR Case Fatality Rate, Y-Chromosome-Bearing Sperms, nematodes, Role Concepts, chromosome scaffold, roundworms, Histone H5, Histone H4, Histone H7, Double-Stranded DNA, deoxyribonucleic acids, DNAn, Age-Specific Death Rate, Family Life Cycles, MST156, NAGR1, Histone H1, Histone H3, ejaculate, nuclear chromatin, Y-Chromosome-Bearing, aberrant, ribose nucleic acid, Modern, ribonucleic acids, Crude Death, Double-Stranded, historical aspects, mKIAA4193, results, hnRNP M, Crude Mortality, Programs, Acute onset, (Deoxyribonucleotide)n+m, Mortality, mislocalized, Role Concept, species., Ribonukleinsaeure, Role, pentosenucleic acids, GENA70, Genetic Materials, Ribonucleic acids, desoxyribose nucleic acid, Filiation, Genetic Material, Acid, X-Chromosome-Bearing, death rate, Epigenetic, mKIAA1341, Mortalities, protein-RNA complex, INSDC_feature:gene, spermatozoid, X Bearing Sperm, human, Life Cycles, seminal fluid, Phenotypes, Mortality Rate, Crude Death Rate, Material, Sperms, historical notes, ds DNA, Hot Temperatures, Cistron, Mammalia, DNA, Mortality Determinant, Epigenetics, humans, accessory, FLORAL DEFECTIVE 10, atypia, cytoplasmic chromatin, HNRPM4, Sperm, human being, DNS, Family Member, (Deoxyribonucleotide)n, ectopic, Aspect, Mortality Declines, mating, Excess, HNRPM, Gene, SUPERMAN, MSTP156, Network, Crude Mortality Rate, protein-containing complex, Y-Chromosome-Bearing Sperm, mortality measurement, supernumerary, Deoxyribonucleic acids, Age-Specific Death, Human, Mortality Determinants, Crude, Hnrpm, Rate, Determinant, Deoxyribonucleic Acid, polypeptide chain, Homo sapiens, resistance, Gene Products, Spermatozoon, Case Fatality, mammals, atypical, Y Bearing Sperm, Man, Histone H3.3, Differential Mortality, study, Ribonucleoprotein, Excess Mortality, Genetic, Research, ligand, Double Stranded, Deoxyribonucleic acid, X-Bearing Sperms, Age-Specific, X-Chromosome-Bearing Sperms, Non-Polyadenylated RNA, nematode, Mif1, sperm, Hnrpm4, Mortality Rates, mortality rate, Crude Death Rates, Chronic, SPERM, (Deoxyribonucleotide)m, species, Kinship Networks, Family, Age Specific Death Rate, myEF-2, 9430071B01, RNA, Nemata, X-Bearing Sperm, Mortality Decline, Family Research, protein complex, Males, DNAn+1, MEF-2, Proteins, Mammal, RNP, RNS, defective, Synaptosomal-associated 25 kDa protein, Historical Aspects, Cistrons, motif, Histone, Historical Aspect, spermatozoon, Concept, RNA-protein complex, Family Members, Case Fatality Rate, HsT18564, survival, native protein, Temperatures, natural protein, yeast nucleic acid, Protein, background, ds-DNA, Determinants, Death, Rhabditis elegans, Hexosamine, Y-Bearing, ribonucleic acid, Rates, Temperature, Death Rate, increased number, Non Polyadenylated RNA, Non-Polyadenylated, FLO10, Ribonucleic Acid, Age-Specific Death Rates, HERP, Histone H1(s), introduction, AA409009, Protein Gene Products, Gene Proteins, present in greater numbers in organism, Differential, Y-Bearing Sperms, Families, SNAP, Modern Man, Desoxyribonukleinsaeure, Decline, Aspects, Relatives, Differential Mortalities, Historical"],"name_synonyms":["Networks, Reproductive, RNA, Ribonucleic, HNRPM4, HTGR1, Family Member, Kinship, Family Research, ribose nucleic acid, CEAR, protein complex, FON1, Proteins, ribonucleic acids, HNRPM, Gene, SUPERMAN, RNS, Synaptosomal-associated 25 kDa protein, Network, protein, FLORAL ORGAN NUMBER 1, protein-containing complex, mKIAA4193, hnRNP M, Family Members, Hnrpm, Subfertility, protein polypeptide chains, native protein, natural protein, polypeptide chain, Reproductive Sterility., yeast nucleic acid, Ribonukleinsaeure, Protein, SUP, Gene Products, GENA70, Life Cycle, pentosenucleic acids, Ribonucleic acids, protein aggregate, Filiation, Non Polyadenylated, RNA Gene Products, Family Life Cycle, ribonucleic acid, Acid, Kinship Network, Research, Epigenetic, Non Polyadenylated RNA, ligand, 2610023M21Rik, Bdr, Non-Polyadenylated, FLO10, proteins, HNRNPM4, Ribonucleic Acid, Super protein, HERP, sp, Sub-Fertility, SNAP-25, Epigenomic, AA409009, Non-Polyadenylated RNA, Protein Gene Products, Life Cycles, Gene Proteins, Mif1, Hnrpm4, Families, SNAP, infertility, Sterility, Kinship Networks, Family Life Cycles, Epigenetics, Family, NAGR1, Relatives, FLORAL DEFECTIVE 10"],"citation_count":["0"],"additional_accession":["PXD006094"]},"is_claimable":true,"name":"C. elegans SUP-46, an HNRNPM family RNA-binding protein that prevents paternally-mediated epigenetic sterility ","description":"BioID screening of HNRNPM, MYEF2 in HEK293 cell line.","dates":{"publication":"Tue Mar 14 15:33:00 GMT 2017"},"accession":"MSV000080631","cross_references":{"pubmed":["28716093"]}}