{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000081638/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Laurence Florens"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=82bab7095b3344129e709570ff9e888f"],"submitter_email":["laf@stowers.org"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["62"],"ptm_modification":["MOD:01060 - \"A protein modification that effectively converts an L-cysteine residue to S-carboxamidomethyl-L-cysteine.\"","MOD:00719 - \"A protein modification that oxygenates an L-methionine residue to one of the diastereomeric L-methionine sulfoxide residues.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["LTQ XL"],"species":["Mus Musculus (ncbitaxon:10090)"],"submitter_affiliation":["The Stowers Institute for Medical Research"],"pubmed_abstract":["Fibroblast growth factor 2 (FGF2) is a well-known cell proliferation promoter; however, it can also induce cell cycle arrest. To gain insight into the molecular mechanisms of this antiproliferative effect, for the first time, the early systemic proteomic differences induced by this growth factor in a K-Ras-driven mouse tumor cell line using a quantitative proteomics approach are investigated. More than 2900 proteins are quantified, indicating that terms associated with metabolism, RNA processing, replication, and transcription are enriched among proteins differentially expressed upon FGF2 stimulation. Proteomic trend dynamics indicate that, for proteins mainly associated with DNA replication and carbohydrate metabolism, an FGF2 stimulus delays their abundance changes, whereas FGF2 stimulation accelerates other metabolic programs. Transcription regulatory network analysis indicates master regulators of FGF2 stimulation, including two critical transcription factors, FOSB and JUNB. Their expression dynamics, both in the Y1 cell line (a murine model of adenocarcinoma cells) and in two other human cell lines (SK-N-MC and UM-UC-3) also susceptible to FGF2 antiproliferative effects, are investigated. Both protein expression levels depend on fibroblast growth factor receptor (FGFR) and src signaling. JUNB and FOSB knockdown do not rescue cells from the growth arrest induced by FGF2; however, FOSB knockdown rescue cells from DNA replication delay, indicating that FOSB expression underlies one of the FGF2 antiproliferative effects, namely, S-phase progression delay."],"pubmed_title":["FGF2 Antiproliferative Stimulation Induces Proteomic Dynamic Changes and High Expression of FOSB and JUNB in K-Ras-Driven Mouse Tumor Cells."],"pubmed_authors":["Vitorino Francisca Nathalia de Luna FNL, Montoni Fabio F, Moreno Jaqueline Neves JN, de Souza Bruno Ferreira BF, Lopes Mariana de Camargo MC, Cordeiro Barbara B, Fonseca Cecilia Sella CS, Gilmore Joshua M JM, Sardiu Mihaela I MI, Reis Marcelo Silva MS, Florens Laurence A LA, Washburn Michael P MP, Armelin Hugo Aguirre HA, da Cunha Julia Pinheiro Chagas JPC"],"pubmed_title_synonyms":["Fgf-2, Malignant Neoplasm, GOS3, Fibroblast Growth Factor-2, Laboratory, FGF 2, RNHIA, Ki-ras, FGF2, AP-1, xralb, Neoplasms, Kras2, Mus domesticus, mouse, Benign Neoplasm, FHF-2, Class II Heparin-Binding Growth Factor, K-ras, GOSB, Basic Fibroblast Growth Factor, rash1, Tumor, hras1, Malignant, Cartilage-Derived Growth Factor, House Mouse, FGFB, JUNB, HBGF-2, fgf2, ras, Benign, fra-2, House, Mus, CTLO, bFGF, Neoplasm, Kras-2, Mus musculus domesticus, Cell., K-Ras, K-RAS, Heparin-Binding Growth Factor Class II, FGF-13, AI929937, C-BAS/HAS, Mice, k-ras, BFGF, Cartilage Derived Growth Factor, C-HA-RAS1, KRAS2, rask2, p21B, Basic, HAMSV, Mus musculus, Malignancy, RNASEHI, H-RASIDX, Swiss, Fibroblast Growth Factor, mice, FHF2, RASH1, Prostate Epithelial Cell Growth Factor, Swiss Mouse, Benign Neoplasms, Prostatropin, House Mice, Swiss Mice, Cancers, domesticus, Laboratory Mice, Malignant Neoplasms, Neoplasias, C-H-RAS, N-RAS, RNHL, G0S3, HRAS1, kras, FGF-2, H-Ras, Mouse, Malignancies, house mouse, AGS4, other neoplasm, Laboratory Mouse, Fgfb, Neoplasia, Cancer, Tumors"],"description_synonyms":["Fgf-2, Qa1, l(4)17, l(4)13, ANT-C, Gli, tumor suppressor, Bhlha41, Scx, SCX, Fibroblast Growth Factor-2, Calf Serum, Cid[Mel], postnatal development, Antp1, leg, Ci[D], classic hairy cell leukaemia, bHLHa48, BOUND WATER, growth and development, protein, Hydrogenchlorid, Basodexan, Serum, Cell Growth in Number, Arrests, CENP-A, HBGF-2, CENP-C, HOH, l(1)AA33, Polypeptides, Cell Cycle-Transition Points, protein polypeptide chains, cell cycle regulator, Fetal Bovine Serum, hydrogen chloride, peptido, scx, bFGF, B1, AntP1, High Performance Liquid Chromatography, Cell Number Growth, 1, 2, Cell Cycle Transition Points, Min, FGF-13, Analysis, protein aggregate, Fs(3)Hor, BFGF, DmelCG42273, Mass Spectrum Analysis, CG13329, Growth, DmelCG2684, BcDNA:RE21270, peptides, Analyses, S-phase, E927b, hypoplasia, min, mAPC, proteins, NTef2, SCAN, xscleraxis, HPLC, BG:DS07700.1, AI047805, reaction, Wasserstoffchlorid, CI, High-Performance, l(1)B2/13.1, Fetal, RUN, Run, T18c(37), bHLHa41, sample, D1, T18c, Chromatography, Cenp-A, dihydridooxygen, ANT-P, High-Performance Liquid, Cell Cycle, High Speed Liquid, necrosis, dihydrogen oxide, Cell Cycle Arrest, 37b, 37c, Ce, l(1)LB9, Ci, Trypsin/K, aqua, Liquid Chromatography, Dmel_CG7826, Th, Bovine Albumin, classic hairy cell leukemia, Cartilage-Derived Growth Factor, Spectrum Analysis, fragmented, glycogen storage disease 11, ur, collisionally activated dissociation, Aus, AA33, Fs(3)Sz11, Cid, CID, ciD, Spectroscopy, carbamide, H2O, batch, Dm1, arrest of mitotic cell cycle progression, BB114693, Fetal Calf Serum, Chlorwasserstoff, Cellular, ci-D, Heparin-Binding Growth Factor Class II, Dmel_CG7835, Hydrogen Oxide, Fetal Calf, cenpA, Mnb, MNB, Arrest, CenH3/CID, Ci/Gli, Ci/GLI, growth pattern, CENP-A/Cid, CENP-A/CID, Fibroblast Growth Factor, non-developmental growth, glycogenosis Fanconi EXACT, CENPA, FHF2, cessation of cell cycle, cracked, HCl, Control, Spectrometry, hydrogen hydroxide, Controls, AW124434, Bovine Serum, beta Trypsin, early, acqua, Cycle-Transition Point, nanospray, H2NC(O)NH2, hepatic glycogenosis with amino aciduria and glucosuria, Proliferation, Horka, Acetamide, Cell Number, regulation of cell cycle arrest, CG2684, Qed-1, Fs(3)Horka, Liquid, Polypeptide, Wasser, Fanconi type, chlorane, GSD type XI, 1728, Ci-155, GLUT2 deficiency, CG1849, Peptidomics, chlorure d'hydrogene, FGF2, fractured, Cell Cycle Control, number, DMANTPE1, Gene, Class II Heparin-Binding Growth Factor, arrest of cell cycle progression, FBS, Fbs, Carbamide, Spectrum Analyses, Antp P1, Antp P2, protein-containing complex, thomson, presence, SCXB, FGFB, CG7826, agua, SCXA, mass-to-charge ratio, fgf2, Karbamid, CenpA, l(4)102ABc, polypeptide chain, reduced, termination of cell cycle, H2-Qa1, Hu, Harnstoff, Mass, Gene Products, CG7835, CG42273, Qa-1(b), DmF2, tiny, Number Growth, Carmol, 3.4, Siah, cloruro de hidrogeno, Mass Spectroscopy, pseudo-phlorizin diabetes, lod, CenH3[Cid], CenH3[CID], ANTC, antp, T23d, Hydrogen chloride, T23b, Runt, Prostate Epithelial Cell Growth Factor, hepatic glycogenosis with Fanconi nephropathy, CiD., beta-Trypsin, DmelCG13329, eau, High-Performance Liquid Chromatographies, CG1028, X-linked combined immunodeficiency, cell proliferation, Bovine, glycogen storage disease type 11, accidental cell death, Ultra Performance Liquid Chromatography, Checkpoint, CG2125, Albumin Bovine, peptidos, Cell Cycle-Transition, UPLC, AntP, ANTP, Fgfb, negative regulation of cell cycle arrest, 2-iodo-, TCEP, PTHB1, LB5, High-Performance Liquid Chromatography, small, Multiplication, DYRK1, Cellular Proliferation, ng/ml, Cell Cycle-Transition Point, FGF 2, cell cycle arrest, protein complex, Proteins, DmelCG1849, split, FHF-2, cenH3, chloridohydrogen, Basic Fibroblast Growth Factor, Rnt, DmAntp, Cell, Peptide, lLB5, development, [HCl], positive regulation of cell cycle arrest, count in organism, MS, Checkpoints, CAD, native protein, Temperatures, natural protein, carbonyldiamide, ci[D], C18, Ci155, glycogen storage disease XI, Protein, Hydrochloride, hemorrhaged, Dyrk1, l(3)84Ba, Cell Cycle Arrests, Death, Bovine Serum Albumin, glycogen storage disease type XI, Cartilage Derived Growth Factor, INSDC_feature:regulatory, Mass Spectrum Analyses, glycogenosis, Basic, AU020952, torn, Mass Spectrum, hepatorenal glycogenosis with renal Fanconi syndrome, Ant, PRSS, acetic acid ammonium salt, DmelCG2125, GSD type 11, CenH3, ci155, underdeveloped, Cell Cycle Controls, uree, CC1, postnatal growth, Tripcellim, Prostatropin, l(1)19Ea, Lds, FBS1, Fbs1, sample population, Cell Cycle Checkpoint, mitotic cell cycle arrest, ME-IV, Protein Gene Products, P235, Gene Proteins, Trypure, Ns, High Pressure, H-2T23, Albumin, hepatorenal glycogenosis with renal fanconi syndrome, DmelCG1028, Point, Fetal Bovine, Cell Multiplication, FGF-2, Qa-1, Peptid, DRO15DC96Z, High Performance Liquid, [OH2], growth, CenpA/CID, hydrochloric acid, Scl, CID/CENP-A"],"name_synonyms":["Fgf-2, Basic, Extract, Fibroblast Growth Factor-2, FGF 2, FGF2, Fibroblast Growth Factor, Blood, FHF2, Prostate Epithelial Cell Growth Factor, FHF-2, Prostatropin, Class II Heparin-Binding Growth Factor, Basic Fibroblast Growth Factor, Blood Serum, Serum, HBGF-2., Cartilage-Derived Growth Factor, Cell, FGFB, fgf2, Cell Extract, bFGF, Extracts, FGF-2, Heparin-Binding Growth Factor Class II, FGF-13, Serums, BFGF, Cartilage Derived Growth Factor, Fgfb"],"pubmed_abstract_synonyms":["Fgf-2, src-1, tumor suppressor, Laboratory, Adenomas, Y1 细胞, lambdatop, CT22273, Metabolic Concepts, Receptors, Y1 cell, dev, growth and development, ADNOS, House Mouse, JUNB, HBGF-2, Long Term, Dm SRC41, DmelCG7223, Cell Cycle-Transition Points, protein polypeptide chains, cell cycle regulator, ras, FGF receptor activity, bFGF, Granular Cell Adenocarcinoma, Post Transcriptional, Line, Cell Cycle Transition Points, Tubular Carcinoma, RNA Modification, Autonomous Replication, Metabolism Concept, Fs(3)Hor, Posttranscriptional RNA Processing, BFGF, Cribriform Carcinoma, Fgf-r, Adenoma, catabolism, metabolic process resulting in cell growth, proteins, NTef2, FGF Receptor Complexes, BTL/FGFR2, Post-Transcriptional RNA Modifications, RNHL, Cell Cycle, transcription from bacterial-type RNA polymerase promoter, Tk2, Tk1, single organism signaling, Tk5, metabolism resulting in cell growth, Modifications, Longterm Effect, Cartilage-Derived Growth Factor, Fs(3)Sz11, fra-2, arrest of mitotic cell cycle progression, HD-29, UM-UC-3, Cellular, secretion, Heparin-Binding Growth Factor Class II, SK2-4, Carcinomas, HD-38, Htl, Fr1, carbohydrate metabolism, EMS2, FHF2, Processing, \"adenocarcinoma\" EXACT [CSP2005:2000-0386], Swiss Mouse, MRE5, c-SRC, i100, Controls, DmelCG7524, Cycle-Transition Point, FR1, Replications, G0S3, HRAS1, Proliferation, CG2684, H-Ras, c-src, Mouse, SK-N-MC, l(3)00208, DNA, AGS4, Adenocarcinoma, GOS3, i79, Effects, Peptidomics, Processes, FGF2, RNA Processing, Cell Cycle Control, number, Su(Raf)1, Class II Heparin-Binding Growth Factor, Dm SRC1, GOSB, Metabolic Processes, protein-containing complex, hras1, Transcription Factor, FGFB, UMUC3, Granular Cell Adenocarcinomas, fgf2, Heparin Binding Growth Factor Receptor, src64, RNA Modifications, single-organism carbohydrate metabolic process, Gene Products, Kras-2, Mus musculus domesticus, no subtype (morphologic abnormality)\" EXACT [SNOMEDCT_2005_07_31:35917007], fibroblast growth factor-activated receptor activity, Number Growth, C-BAS/HAS, DNA Replications, Modification, network topology analysis, Transcription, multicellular organismal carbohydrate metabolic process, Src42a, Longterm, Prostate Epithelial Cell Growth Factor, Tubular Adenocarcinoma, Metabolic Concept, Long-Term, CG6714, tumour cell, Granular Cell Carcinomas, FGFR, DSrc64, Checkpoint, dtk-5, pp60c-src, Fgfb, negative regulation of cell cycle arrest, DSRC64, RNA, FGF Receptors, Cell Cycle-Transition Point, degradation, FGF 2, Basal Cell, CG44128, Proteins, j372, dtk5, Factor, Basic Fibroblast Growth Factor, SKNMc, dtk2, rash1, dtk1, Cell, Concept, positive regulation of cell cycle arrest, Posttranscriptional RNA, Checkpoints, \"adenocarcinoma, fgf-r, native protein, CTLO, Long Term Effects, Post-Transcriptional RNA, i150, Granular Cell, CG7873, Cell Cycle Arrests, metabolism, Dsrc42A, INSDC_feature:regulatory, Metabolic Phenomenon, multicellular organism metabolic process, l(2)k10108, Src, Post Transcriptional RNA Processing, Posttranscriptional, DFR1/DFGF-R2, postnatal growth, AW259666, Prostatropin, House Mice, Lds, NICR2106, Malignant Adenomas, Longterm Effects, Cell Cycle Checkpoint, mitotic cell cycle arrest, CG32134, CT20816, Gene Proteins, Post-Transcriptional, src, signalling process, Point, src42, Su1, Fibroblast Growth Factor Receptor, FGF Receptor Complex, Receptor, Dtk1, Dtk2, Dtk5, Dsrc64B, Anabolism, biochemical pathways, Dfr1, Metabolic Process, Fibroblast Growth Factor-2, AP-1, postnatal development, Mus domesticus, pp60v-src, dFGFR, protein, Cell Growth in Number, Arrests, DFGF-R2, \"adenocarcinoma\" EXACT [NCI2004_11_17:C2852], DFGF-R1, Cell Number Growth, Concepts, FGF-R2, FGF-13, Post-Transcriptional RNA Processing, protein aggregate, Phenomenon, Effect, FGF Receptor, Dmel_CG7873, k-ras, Btl, DFR2, DFR1, rask2, Growth, DmelCG2684, FGF-activated receptor activity, tumor cell, H-RASIDX, Granular Cell Carcinoma, src64B, CG7524, CT39172, xsrc, Swiss Mice, Tubular Carcinomas, Dfr-1, Post Transcriptional RNA Modification, Dfr-2, Autonomous, ASV, N-RAS, DFR-1, biotransformation, house mouse, Cell Cycle Arrest, Catabolism, Long-Term Effects, Carcinoma, Process, Ki-ras, xralb, csrc, mouse, Post-Transcriptional RNA Modification, DmHD-29, Fibroblast Growth Factor Receptors, CG7223, Adenocarcinomas, Programs, Su(phl)1, DmelCG44128, Tubular, Heparin-Binding Growth Factor Receptor, Malignant Adenoma, Basal Cell Adenocarcinoma, SRC 64B, Su(D-raf)1, Lines, Mus musculus, Arrest, Factors, growth pattern, Src1, SRC1, DmelCG32134, Fibroblast Growth Factor, non-developmental growth, mice, dSrc, cessation of cell cycle, Control, Cribriform, Tubular Adenocarcinomas, early, domesticus, Horka, bacterial transcription, Cell Number, regulation of cell cycle arrest, Fs(3)Horka, Autonomous Replications, DmHD-38, D-Src64B, Y1, src1, src2, DTRK(FR1), Dsrc, RNHIA, Dsrc41, Kras2, D-src, Oxyphilic Adenocarcinoma, CG14471, C-src1, Gene, arrest of cell cycle progression, Malignant, presence, DSrc, polypeptide chain, DmHD-358, House, termination of cell cycle, Metabolism, susceptible, dsrc, DmF2, Basal Cell Adenocarcinomas, p60-Src, Mice, Metabolism Phenomena, KRAS2, lod, p21B, Src64, 0844/01, RNASEHI, Swiss, Dsrc64, S-phase., RASH1, Cribriform Carcinomas, cell proliferation, Long-Term Effect, Cell Cycle-Transition, SRC 42A, HD-358, SRC42A, DSrc64B, FGFR2, FGFR1, Multiplication, Oxyphilic, Src42, Cellular Proliferation, Src41, cell cycle arrest, protein complex, DPR3, src42a, Cell Lines, D-FGFR, FHF-2, K-ras, Metabolic Phenomena, development, Metabolism Concepts, count in organism, FGF, natural protein, Mus, DmHD-311, Protein, src42A, Phenomena, src64b, K-Ras, K-RAS, Oxyphilic Adenocarcinomas, AI929937, Replication, Cartilage Derived Growth Factor, C-HA-RAS1, DSrc42A, Basic, HAMSV, NICR502, biodegradation, Metabolic, Cell Cycle Controls, Dmel_CG14471, Carbohydrate, \"adenocarcinomas\" EXACT [SNOMEDCT_2005_07_31:189578007], HTL/FGFR1, Laboratory Mice, Protein Gene Products, C-H-RAS, kras, Cell Multiplication, FGF-2, HD-311, \"adenocarcinoma NOS (morphologic abnormality)\" EXACT [SNOMEDCT_2005_07_31:189582009], growth, Laboratory Mouse, fibroblast growth factor receptor activity"],"citation_count":["0"],"additional_accession":["PXD008026"]},"is_claimable":true,"name":"MudPIT analyses of whole cell extracts from Y1 murine adrenocortical carcinoma cell line stimulated with serum or FGF2","description":"Fibroblast growth factor 2 (FGF2) is well known as a promoter of cell proliferation, however, in some cellular contexts, it can induce cell cycle arrest or cell death. In Y1 murine adrenocortical carcinoma cell line, FGF2 induces G0/G1 transition but delays S-phase progression and permanently block cells in G2/M. To gain insights into the molecular mechanisms of this antiproliferative effect, we investigated the early proteomics alterations induced by this growth factor. We compared mass spectrometry-based quantitative proteomics analyses of Y1 cells stimulated, after 3 and 5 hours, with fetal bovine serum (FBS) or FGF2. \nTotal protein extracts from Y1 cells stimulated with FBS or FBS plus FGF2 (10 ng/ml) for 0 h, 3 h, and 5 h were obtained in 3 biological replicates. One hundred and fifty micrograms of proteins were TCA precipitated and dissolved in 100 mM Tris-HCL pH 8.5, 8 M urea. The sample was reduced with 5 mM TCEP, incubated at room temperature for 30 min, and carboxymethylated by the addition of 10 mM of iodoacetamide. Proteins were digested with endoproteinase Lys-C for 4 h and then digested with Trypsin overnight at 37C. The peptides were loaded onto a 100 um three-phase capillary column packed with 8 cm of 5 um C18 reverse phase (Aqua; Phenomenex), followed by 3 cm of 5 um SCX resin (Partisphere SCX; Whatman) and an additional 1.5 cm of reverse phase. Columns loaded with the peptides were placed in-line with an Agilent 1100 quaternary HPLC connected with a LTQ-XL (Thermo) mass spectrometer equipped with a nanospray ionization source. Twelve automatic steps were used in the MudPIT run with increasing concentrations of ammonium acetate. Each full MS scan (400 m/z to 1600 m/z) was followed by 5 MS/MS events fragmented by CID. ","dates":{"publication":"Mon Oct 23 08:05:00 BST 2017"},"accession":"MSV000081638","cross_references":{"pubmed":["30035358"]}}