MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v01/MSV000081727/primaryOK2000000ProteomicsElizabeth YohannesLTQ OrbitrapHomo Sapiens (ncbitaxon:9606)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=c99afa76aab4409780bdcccb417e66e4elizabeth.h.yohannes@gmail.comMADIGA Army Medical CenterMassIVE47MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetHydrosalpinx, the blockage of fallopian tubes, can result from pelvic inflammatory disease. Hydrosalpinx is a cause of infertility and negatively impacts in vitro fertilization. To better understand the pathobiology of hydrosalpinx, we compared the proteome of lavages from disease vs. healthy fallopian tubes. Results indicate a disruption of redox homeostasis and activation of the complement system, immune cell infiltration, and phagocytosis; pathways that may drive tubal injury. To our surprise among the most prominent proteins with hydrosalpinx was mesothelin (MSLN), which until now has only been associated with epithelial malignancies. Analogous to mesothelioma and ovarian carcinoma, a significant increase of MSLN was detected in plasma from patients with hydrosalpinx. This finding suggests MSLN may provide clinical diagnosis in lieu of the current approaches that require invasive imaging. Importantly, these findings implicate MSLN in a benign disease, indicating that the activation and role of MSLN is not restricted to cancer.The human tubal lavage proteome reveals biological processes that may govern the pathology of hydrosalpinx.Yohannes Elizabeth E, Kazanjian Avedis A AA, Lindsay Morgan E ME, Fujii Dennis T DT, Ieronimakis Nicholas N, Chow Gregory E GE, Beesley Ronald D RD, Heitmann Ryan J RJ, Burney Richard O ROprojections, Irrigation, Douchings, Therapeutic Irrigations, human being, Man (Taxonomy), histopathology, lamellae, shelves, Modern, lamina, total expressed protein, flanges, ridges, Lavages, projection, process of organ, ridge, human, Douching, Human, Lavage, lamella, processes, Homo sapiens, Therapeutic, papilla, Modern Man, shelf, cytopathology, biopsy, Pathologies., Irrigations, laminae, Man, Proteomes, flange, humans, organ processbig, other disease, Mammalian Oviduct, Peptidomics, Fallopian tube, Uterine Tube, non Brugada type, Mammalian, Salpinx, Test Tube Fertilization, Gene, tuba uterina, 116, side effects, fluid, CMD1E, paroxysmal familial ventricular fibrillation (disorder), Test-Tube Baby, prevention, CDCD2, ventricular fibrillation during myocardial infarction, large, paroxysmal familial, diseases, Uterine Salpinx, Babies, HH1, Gene Products, disease or disorder, diseases and disorders, prevention and control, Irrigation, ART, human disease, Therapeutic Irrigations, PFHB1, reference sample, 2310061K05Rik, Test-Tube Babies, Mammalian Oviducts, Fallopian Tube, Oviduct, non-neoplastic, ventricular fibrillation, preventive measures, Salpinges, oviducts, anatomical tube, great, Oviducts, disorder, Nav1.5, Homo sapiens disease, HB1, fallopian tubes, salpinx, HB2, Irrigations, Uterine Salpinges, Controlled, VF1, Tube, Douchings, Controlling, preventive therapy, LQT3, Test-Tube Fertilizations, female paramesonephric duct, Proteins, disorders, Test Tube Babies, In Vitro Fertilizations, Test-Tube, In Vitro Fertilization, medical 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inhibition of homeostatic process, mesothelioma, Sub-Fertility, Salpinges, Immune, malignant neoplasm, infertility, Role Concepts, Oviducts, homeostasis, Homo sapiens disease, Malignancies, Irrigations, Adnexitis, plasma, CAK 1 Antigen, Diagnostic Findings, Tumors, close to, SIGNS SYMPTOMS, screening, Tube, Douchings, Frozen Plasma, Megakaryocyte, female paramesonephric duct, Injury and Wounds, DESC, Test-Tube, Physical Traumas, Tubes, Fertilizations in Vitro, Plasmas, Uterine, Douching, results, Role Concept, Benign, positive regulation of homeostatic process, Potentiating Factor, epithelial, Megakaryocyte Potentiating Factor, Pelvic Inflammatory Diseases, Diseases, Role, Physical Trauma, Phagocytoses, activation, Ovarian carcinoma, Drives, System, signs, Benign Neoplasms, paramesonephric duct of female, pelvic inflammatory, Lavages, Fallopian, Lavage, disease, Fertilization, Baby, Patient, Research-Related Injuries, Inflammatory Diseases, Research-Related Injury, Clinical Finding, Proteomes, Malignant Neoplasms., Fresh, Research Related Injuries, MPF, other disease, Pelvic Disease, Mesotheliomas, Mammalian Oviduct, Fresh Frozen Plasma, Uterine Tube, Neoplasms, Benign Neoplasm, Test Tube Fertilization, Gene, tuba uterina, Inflammatory Pelvic Disease, Malignant, Test-Tube Baby, Injuries and Wounds, Symptoms and Signs, Subfertility, Pelvic Inflammatory Disease, Wounds, Uterine Salpinx, Gene Products, disease or disorder, CAK-1 Antigen, Finding, Inflammatory Pelvic, Irrigation, Blood Plasma, Therapeutic Irrigations, Injury, Malignancy, Test-Tube Babies, Mammalian Oviducts, Fallopian Tube, inflammatory disease (PID), Inflammatory Disease, Oviduct, non-neoplastic, Neoplasias, Inflammatory Pelvic Diseases, pelvic infection, PID, Traumas, Clients, Reproductive Sterility, Blood Plasmas, negative regulation of homeostatic process, disorder, fallopian tubes, salpinx, Inflammatory, pelvic, Uterine Salpinges, Cancer, Autoregulation, Reproductive, Disease, findings, Malignant Neoplasm, Test-Tube Fertilizations, Proteins, Test Tube Babies, disorders, total expressed protein, In Vitro Fertilizations, In Vitro Fertilization, medical condition, Factor, Client, Concept, near to, Frozen Plasmas, Signs and Symptoms, MT, traumatic injury, Wounds and Injury, Megakaryocyte Potentiating, Trauma, Systems, Protein, Neoplasm, condition, salpinges, regulation of homeostatic process, Mes, epitheliocyte, Plasma, mammalian oviduct, primary cancer, wound, ovarian epithelial cancer, portion of plasma, uterine tube (sensu Mammalia), Cancers, malignant tumor, Injuries, CAK-1, Protein Gene Products, Gene Proteins, activation of homeostatic process, Therapeutic, Uterine Tubes, approaches, pelvic inflammatory disease, Fertilizations, Sterility, vicinity of, Test-Tube Fertilization, Pelvic, Neoplasia0falseShotgun proteomics Identifies Molecular Signatures of HydrosalpinxSubsequent to pelvic inflammatory diseases fallopian tube developed Hydrosalpinx, which is characterized as an enlarged occluded tube filled with fluid. Hydrosalpinx has adverse effects on tubal physiology and has been detrimental to success of in vitro fertilization (IVF). To assess molecular alteration associated with the effect of hydrosalpinx, we have performed discovery shotgun proteomics and verification analyses on 30 hydrosalpinx and healthy control lavages. These analyses distinguished 116 differentially expressed proteins in hydrosalpinx relative to healthy lavage. Mon Nov 20 15:38:00 GMT 2017MSV00008172731222072