{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000082209/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Bienyameen Baker"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=7b927c745be04c67a0b0924bf528cf5f"],"submitter_email":["brubaker@sun.ac.za"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["92"],"ptm_modification":["MOD:00110 - \"A protein modification that effectively converts an L-cysteine residue to L-cysteine methyl disulfide.\"","UNIMOD:35 - \"Oxidation or Hydroxylation.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["Q Exactive"],"species":["Mycobacterium Tuberculosis (ncbitaxon:1773)"],"submitter_affiliation":["Stellenbosch University"],"pubmed_abstract":["The emerging resistance of tuberculosis (TB) to current first line drugs (isoniazid, rifampicin, pyrazinamide, ethambutol) warrants alternative treatment approaches with broad-spectrum efficacy. Previously, we have shown that sulfamethoxazole (SMX) has synergestic activity with rifampicin against Mycobacterium tuberculosis. The primary target of SMX is folP1 in mycobacteria; however, SMX may affect other secondary targets in M. tuberculosis. This study investigated the potential additional targets of SMX in a clinical isolate of M. tuberculosis using Orbitrap mass spectrometry to identify differentially expressed proteins following treatment with a sub-lethal concentration of SMX. Raw data have been deposited as ProteomeXchange accession PXD009315. Our proteomic analysis identified approximately 1500 proteins in total of which 45 proteins were differentially regulated as a result of SMX treatment. These included 25 upregulated and 20 downregulated proteins. The oxidative stress proteins (Rv2428, AhpC and Rv2394, GgtB) and an enzyme from the electron transport chain (Ndh-II, Rv1854c) were found to be upregulated. Gene expression analysis correlated with the observed proteomic changes. In conclusion our results show that SMX treatment of a drug sensitive M. tuberculosis clinical isolate resulted in the regulation of proteins involved in the oxidative stress response, indicating the induction of oxidative stress by SMX in mycobacteria."],"pubmed_title":["Proteomic analysis reveals that sulfamethoxazole induces oxidative stress in M. tuberculosis."],"pubmed_authors":["Sarkar R R, Mdladla C C, Macingwana L L, Pietersen R-D RD, Ngwane A H AH, Tabb D L DL, van Helden P D PD, Wiid I I, Baker B B"],"pubmed_title_synonyms":["DNA Oxidative, Oxidative Stress, Kochs Disease, Antioxidative, Antioxidative Stress, Nitrative Stress, Anti-oxidative Stresses, Sulfamethylisoxazole, Damage, Stresses, Oxidative Nitrative Stress, Oxidative Nitrative, Oxidative DNA, Oxidative, Stress Injury, Oxidative and Nitrosative Stress, Oxidative Nitrative Stresses, Nitro-Oxidative Stress, proteomic analysis, Infection, Oxidative Cleavages, Tuberculosis, DNA Oxidative Damages, Nitro-Oxidative Stresses, Tuberculoses, Oxidative Injury, DNA Damage, Oxidative Damages, Mycobacterium tuberculosis., Koch's Disease, Koch Disease, Oxidative Injuries, Injury, Anti-oxidative Stress, DNA Oxidative Damage, Infections, Benzenesulfonamide, Oxidative Cleavage, 4-amino-N-(5-methyl-3-isoxazolyl)-, Cleavage, Oxidative DNA Damages, Mycobacterium tuberculosis Infections, Anti-oxidative, Nitro-Oxidative, Oxidative Stress Injuries, Oxidative Stresses, Anti oxidative Stress, Oxidative DNA Damage, Antioxidative Stresses, Stress, Sulfisomezole, Oxidative Stress Injury, Mycobacterium tuberculosis, DNA, Mycobacterium tuberculosis Infection, Nitro Oxidative Stress, Sulphamethoxazole, active tuberculosis, Oxidative Damage, Gantanol"],"description_synonyms":["70zpep, 5730502P04Rik, RS12, PEP/X4, Isonex, SesB, PP14, A/A-T/sesB, Raw, Product, Laboratory, GPATCH6, LYP2, PNT-P1, LYP1, Ftivazide, Irip, EY3-1, pyridine-4-carboxylic acid hydrazide, MS-GFDB, heavy chain disease, NAALAdase, DmelCG6143, ran, prevention, p39B, jive, l(1)AA33, 5730420M11Rik, Myo, DMU42699, NAALADase I, protein polypeptide chains, FOLH, Method, Mycobacterium tuberculosis var. hominis, isonicotinohydrazide, 1, 2, C530001K22Rik, Isonicotinsaeurehydrazid, 2-amino-3-mercaptopropanoic acid, Research Activity, MOV34, D-ets-2, 3520, Laboratory Research, SMC-2, Republic of South Africa, Priorities, DmelCG9999, Bacillus tuberculosis, Rpn7, LyP, somitogenic mesoderm, Bacterium tuberculosis, SET, C, l(3)s12, Membrane glutamate carboxypeptidase, Myo2, RPN8, Rpn8, anon-WO02059370.55, Acid Vanillylidenehydrazide, DROMHC, composition, proteins, procedures, Serotonin 1D beta receptor, DmelCG4299, NMMII, LYP, set, Lymphoid phosphatase, l(1)B2/13.1, medicine, anatomical tube, sample, rpn7, isonicotinic acid hydrazide, ran-1, F12B17_170, DmAAF34715, Myo II, LC-MS2, l(1)LB9, preventive therapy, Mycobacterium tuberculosis (Zopf 1883) Lehmann and Neumann 1896 (Approved Lists 1980), unsegmented mesenchyme, Research Priorities, Procedure, PEPINO, SH2-B PH domain-containing signaling mediator 1, xran, GIG27, DmelCG42865, count, (2R)-2-amino-3-sulfanylpropanoic acid, ADP/ATP translocase, Research and Development, DLK, pntP2, bcgn, Pointed-P1, HLA-DR-associated protein II, l(1)S12, DI-2, I-2Dm, FGCP, Ets94F, F20B18_110, Methodological, 5-HT1DB, Isoniazid, dRanGAP, GD, experimental procedures, Activities, I-2PP1, Pro-rich, TAF-IBETA, EM12, liquid chromatography-tandem mass spectroscopy, Dmel_CG13885, duct, Manuscripts, Mycobacterium Lehmann and Neumann 1896 (Approved Lists 1980), CG3533, TAF-Ibeta, MyoII, Mycobacterium tuberculosis Infection, PEP, content., Smc2l1, CG3416, 0998/12, DBA9, Peptidomics, PRSC_DROME, L-Zystein, dran, protein-containing complex, pep, ara24, fs(2)eo6, DmelCG7620, DMPOINT1A, Sd-RanGAP, Sd-RanGap, CG30170, Dm nmII, Publication, LC-MSMS, Gene Products, 3.1, Technique, ZIPK, F15E12_6, pointed-RC, ZC3HDC9, 2310061K05Rik, Myo-II, zip/MyoII, E-920, A/A-T, 2pp2a, Hcys, CG10331, EK3-2, CG10574, CG10330, Study, l(3)07825, drugs, 2PP2A, Peking, CYSTEINE, dSET, dSet, FREE CYSTEINE, CG17077, Mycobacterium tuberculosis, Development and Research, 4-Pyridinecarboxylic acid, DmnmII, Tubazide, (2R)-2-amino-3-mercaptopropanoic acid, ZIP3, LB5, Pharmaceuticals, GPATC6, Products, Ets, ZC3H9, grupo, Kochs Disease, TRH, Trh, Proteins, ran10A, D10Wsu136e, b(2)cgn, CG7620, E(br), Rnt, compositionality, lLB5, Experiment, native protein, nmMHC, I-2PP2A, chemical analysis, Dm I-2, Strain, S10, Mhc-c, S12, segmental plate, Mopsm, Sh2bpsm1, pnt-P1, dJ142L7.2, pnt-P2, ATCMPG1, MET, ATCMPG2, Col4a-1, ANT, Pharmaceutic Preparations, ensemble, Ptpn8, l(1)9Ed, prophylaxis, Benzenesulfonamide, RanGap1, s10, (R)-2-amino-3-mercaptopropanoic acid, CG13885, primary structure of sequence macromolecule, 2-Amino-3-mercaptopropionic acid, Folate hydrolase 1, ADT2, plan specification, Gene Proteins, 2-PHOSPHOENOLPYRUVIC ACID, control, Isonicotinic Acid, orangi, structure, Sulfisomezole, BcDNA:SD27354, liquid chromatography tandem mass spectrometry, E920, PE, AI450383, CG6883, PTPN8, Pharmaceutical Products, Sulphamethoxazole, POINT, CG8705, liquid chromatography tandem mass spectroscopy, SH2-Bb, BcDNA:RH29995, Bru, IPP2A2, E 920, Activity, MHC, determination, DmelCG30170, b(2)gcn, leg, l(2)k08003, DmelCG17077, atado, protein, Pnt, composed of, Techniques, ranGap, ranGAP, l(1)9Fa, png, Pharmaceutical Product, Pop, Tuberculosis, Cystein, 3.4.17.21, l(1)DC701, AAF30287, protein aggregate, prevention and control, Pnt-P1, Koch's Disease, Svc, SH2-B, AI255214, Koch Disease, DmelCG3533, rangap, LCMSMS, DmelCG3416, reference sample, HTR1DB, TAF-I, MUB3_18, cisteina, 5-HT-1D-beta, SD, RpS12E, X4/PEP, PEPPER, 4-pyridinecarbohydrazide, CG12352, NAALAD1, MUB3.18, Folylpoly-gamma-glutamate carboxypeptidase, isonicotinic hydrazide, preventive measures, IGAAD, CG16944, Methodological Studies, DmelCG10574, RUN, Run, Pharmaceutical, 5-HT-1B, PrEP, NMM, Sd, Strains, Research Priority, PAEG, Dmel_CG6883, fs(2)eo-C, HTR1D2, Serotonin receptor 1B, phapii, Tube, CG6143, CT28175, PRO, hydrazide, LC-MS/MS, StF-IT-1, Mhc-c[1], ben(2)gcn, D-Ets-2, ets94F, 0123/09, myoII, CYS, Cys, results, AA33, SGA-113M, TUBE, HDC07747, HCD, PSM, Psm, 3.1.3.48, SMC2L1, Pharmaceutic, bHLHe13, Prostate-specific membrane antigen, presumptive somite mesoderm, and GLY protein 2, CG11271, Rasl2-8, 2-(phosphonooxy)-2-propenoic acid, PEST-domain phosphatase, and GLY protein 1, Pteroylpoly-gamma-glutamate carboxypeptidase, ses B, F20B18.110, CAPE, P40, dANT, p44S10, AI047692, CG4299, Methodological Study, KIAA1847, L-Cystein, ptd, PntP2, Osi, GdA, GdF, PNBP, Acetamide, l(1)G0126, l(1)G0247, PntP1, E(E2F)3D, PSMA, CG9999, PNTP2, p44s10, GdS, Strains and Sprains, i2pp2a, PNTP1, cg10331, active tuberculosis, Isonicotinic Acid Hydrazide, Gruppe, ZIP, Zip, CG1849, SH2 domain-containing protein 1B, Hydrazide, Procedures, experimental, ETS2, Ets2, unsegmented paraxial mesoderm, gcn, Gene, Myo-II HC, somitomeric mesoderm, GCPII, PHAPII, LC-MS-MS, RanGAP, p42A, l(1)G0386, zip, CAP-E, method, pntegfr, Mycobacteria, 0608/07, polypeptide chain, DmelCG1404, method used in an experiment, susceptible, Studies, N-acetylated-alpha-linked acidic dipeptidase I, F15E12.6, mKIAA1299, Del(8)44H, Sprains, CG15792, PHOSPHOENOLPYRUVATE, Drugs, Union of South Africa, SH2B, methods, Infections, Research, experimental section, Runt, DmelCG11271, ipp2a2, ANT1, 4-amino-N-(5-methyl-3-isoxazolyl)-, mGCP, Mycobacterium tuberculosis Infections, Fin16, Table, Phthivazid, Vanillylidenehydrazide, taf-ibeta, grupos, PASTICCINO 2, DmelCG12352, BGCN, Preparation, DmelCG16944, l(3)j1B7, 2-iodo-, Controlled, l(2)02957, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, span, Controlling, CG42865, protein complex, Isonicotinic Acid Vanillylidenehydrazide, Bgcn, Sprain, DmelCG1849, igaad, Medications, Sulfamethylisoxazole, Phthivazide, gsp1, OK/SW-cl.81, CG1404, group, GCP2, Isonicotinic, Glutamate carboxypeptidase II, isoniazid, TC4, tc4, LC/MS/MS, Cell growth-inhibiting gene 27 protein, Priority, l(3)S12, natural protein, l(1)EM31, Protein, Research Activities, AI551343, I2PP2A, Infection, sequence, p100, techniques, Tuberculoses, BP1081, isonicotinoylhydrazide, l(3)s118306, Zystein, Data Base, DmelCG15792, nzip, F12B17.170, l(1)G0075, PH and SH2 domain-containing signaling mediator, AW545314, ARA24, 2-amino-3-sulfanylpropanoic acid, Mycobacterium tuberculosis H37Rv, Rest, L-2-Amino-3-mercaptopropionic acid, fs(2)eoQS2, l(1)19Ea, 5-HT1B, sample population, Drug, Protein Gene Products, P235, dSET/TAF-Ibeta, Preparations, Ets58AB, 2610030F17Rik, Hmr, l(3)10512, AI425885, Gsp1, l(3)s2783, Dronm-MII, anon-WO0140519.37, assay, AA407739, Runnings, groupe, Scl, zpr, Mycobacterium tuberculosis typus humanus, Pharmaceutical Preparation, Ran, methodology, nmy-2, Gantanol"],"name_synonyms":["DNA Oxidative, Oxidative Stress, Kochs Disease, Antioxidative, Antioxidative Stress, Nitrative Stress, Anti-oxidative Stresses, Sulfamethylisoxazole, Damage, Stresses, Oxidative Nitrative Stress, Oxidative Nitrative, Oxidative DNA, Oxidative, Stress Injury, Oxidative and Nitrosative Stress, Oxidative Nitrative Stresses, Nitro-Oxidative Stress, proteomic analysis, Infection, Oxidative Cleavages, Tuberculosis, DNA Oxidative Damages, Nitro-Oxidative Stresses, Tuberculoses, Oxidative Injury, DNA Damage, Oxidative Damages, Mycobacterium tuberculosis., Koch's Disease, Koch Disease, Oxidative Injuries, Injury, Anti-oxidative Stress, DNA Oxidative Damage, Infections, Benzenesulfonamide, Oxidative Cleavage, 4-amino-N-(5-methyl-3-isoxazolyl)-, Cleavage, Oxidative DNA Damages, Mycobacterium tuberculosis Infections, Anti-oxidative, Nitro-Oxidative, Oxidative Stress Injuries, Oxidative Stresses, Anti oxidative Stress, Oxidative DNA Damage, Antioxidative Stresses, Stress, Sulfisomezole, Oxidative Stress Injury, Mycobacterium tuberculosis, DNA, Mycobacterium tuberculosis Infection, Nitro Oxidative Stress, Sulphamethoxazole, active tuberculosis, Oxidative Damage, Gantanol"],"pubmed_abstract_synonyms":["Rimactane, Ndh syndrome, DNA Oxidative, etambutol, Bru, Isonex, Product, Activity, Raw, 7-diethyl-3, Ftivazide, 4-GalT1, Nitrative Stress, pyridine-4-carboxylic acid hydrazide, Damage, Social Controls, SUB, Transcript Expression Analysis, Oxidative DNA, DmelCG12298, Oxidative, isonicotinohydrazide, Mycobacterium tuberculosis var. hominis, Pharmaceutical Product, Nitro-Oxidative Stress, Gene Expression Monitorings, 2, Tuberculosis, SCRAMBLED, Isonicotinsaeurehydrazid, Nitro-Oxidative Stresses, Analysis, Profilings, Oxidative Injury, KIF20A, Formal Social Controls, DNA Damage, 2-ethanediyldiimino)bis-, 7S)-2, Koch's Disease, Mass Spectrum Analysis, treatment, Bacillus tuberculosis, Svc, Koch Disease, Bacterium tuberculosis, Oxidative Injuries, Analyses, Etambutol Llorente, Moods, b4Gal-T1, Acid Vanillylidenehydrazide, 1-Butanol, Oxidative Cleavage, S, Ethambutol Hydrochloride, 4-pyridinecarbohydrazide, Oxidative DNA Damages, Anti-oxidative, isonicotinic hydrazide, with congenital hypothyroidism, Social, Gene Expression Analysis, Oxidative Stress Injuries, Oxidative Stresses, S-ethambutol, medicine, Pharmaceutical, disease management, Therapies, (S-(R*, isonicotinic acid hydrazide, Benemycin, Rifampin, S-Ethambutol, Therapy, Mycobacterium tuberculosis (Zopf 1883) Lehmann and Neumann 1896 (Approved Lists 1980), (2S, 4-GalT, Heat Shock Protein, hydrazide, Etambutol, STRUBBELIG, Monitorings, beta-1, EMB, Spectrum Analysis, RFP, Ggtb2, Spectroscopy, Gene Expression, 6-diazaoctane-1, Oxidative and Nitrosative Stress, Pharmaceutic, DNA Oxidative Damages, EMB Fatol, Mycobacteria., Transcriptome Profilings, Spectrometry, Control, Controls, Treatments, Isoniazid, Nitro-Oxidative, metastatic, Gene Expression Analyses, Biocatalyst, Oxidative Stress Injury, Expression Analysis, Pyrazinecarboxamide, DNA, Mycobacterium tuberculosis Infection, Ethambutol, EMB-Fatol, Myambutol, Regulation, active tuberculosis, Isonicotinic Acid Hydrazide, Oxidative Damage, Tisamid, Miambutol, (+)-ethambutol, AT1G11140, Regulations, Oxidative Stress, Hydrazide, Stress Proteins, Llorente, Biocatalysts, Antioxidative, Antioxidative Stress, Gene, N'-bis(1-(hydroxymethyl)propyl)ethylenediamine, Spectrum Analyses, Rimactan, CG12298, Gene Expression Profilings, Stresses, rifamcin, mRNA Differential Displays, Mycobacteria, Gene Expression Pattern Analysis, Oxidative Nitrative, Stress Injury, EMB Hefa, (S, Mass, Gene Products, Oxidative Cleavages, Mood, Del(8)44H, Oxidative Damages, Mass Spectroscopy, Drugs, Rifadin, study, Etibi, Heat Shock Proteins, Injury, neonatal, Infections, Rifamycin, mei-1794, Ggtb, Rifampicin, NDH, 4-amino-N-(5-methyl-3-isoxazolyl)-, Transcriptomics, Transcript Expression, Transcriptome Analysis, Mycobacterium tuberculosis Infections, Stress Protein, Monitoring, R*))-, drugs, Enzyme, B-1, Phthivazid, Anti oxidative Stress, Vanillylidenehydrazide, 3-(((4-Methyl-1-piperazinyl)imino)methyl)rifamycin SV, Oxidative DNA Damage, Antioxidative Stresses, GGTB, 2'-(1, Mycobacterium tuberculosis, 2'-(ethylenediimino)di-1-butanol, SRF9, 4-Pyridinecarboxylic acid, Preparation, Tubazide, Expression Analyses, Dexambutol, Pharmaceuticals, (+)-N, rifampicin, Products, Profiling, Transcriptome, Kochs Disease, (+)-S, Formal Social Control, Isonicotinic Acid Vanillylidenehydrazide, Affects, Proteins, Tubocin, Medications, Anti-oxidative Stresses, Sulfamethylisoxazole, Phthivazide, Isonicotinic, STRUBBELIG-RECEPTOR FAMILY 9, Differential Display, isoniazid, Oxidative Nitrative Stress, MS, Transcript Expression Analyses, rifampicinum, ethambutolum, Social Control, Oxidative Nitrative Stresses, Protein, 8-diol, proteomic analysis, Infection, Hydrochloride, Heat-Shock Protein, Tuberculoses, mRNA Differential Display, isonicotinoylhydrazide, Mass Spectrum Analyses, S)-ethambutol, Mass Spectrum, GalT, Gene Expression Monitoring, EMB-Hefa, Col4a-1, Anti-oxidative Stress, Transcriptome Profiling, Pharmaceutic Preparations, diabetes mellitus, mRNA, DNA Oxidative Damage, Benzenesulfonamide, Mycobacterium tuberculosis H37Rv, Dub, beta4Gal-T1, Differential Displays, Cleavage, Transcriptome Analyses, Drug, Protein Gene Products, neonatal diabetes-congenital hypothyroidism-congenital glaucoma-hepatic fibrosis-polycystic kidneys syndrome, Gene Proteins, Preparations, rifampicina, 3-(((4-methyl-1-piperazinyl)imino)methyl)-, Therapeutic, concentration, Isonicotinic Acid, (+)-2, Stress, Sulfisomezole, Treatment, regulation, ethambutol, Pharmaceutical Products, Nitro Oxidative Stress, T19D16.8, Sulphamethoxazole, General activity, SCM, Pharmaceutical Preparation, Mycobacterium tuberculosis typus humanus, Gantanol"],"citation_count":["0"],"additional_accession":["PXD009315"]},"is_claimable":true,"name":"Proteomic analysis reveals that Sulfamethoxazole induces oxidative stress in M. tuberculosis","description":"These sixteen RAW files represent twelve different samples submitted to the Centre for Proteomic and Genomic Research in Cape Town, South Africa, for shotgun proteomics analysis.  Two different strains of Mycobacterium tuberculosis are included:\n\n--K636, a \"Beijing\" variant susceptible to TB drugs that was isolated in Worcester, Western Cape, South Africa\n--SAWC1129, an M.tb variant that is resistant to isoniazid, isolated in the Western Cape, South Africa\n\nEach of these two strains were split to two cohorts, one subjected to sulfamethoxazole (SMX) and another that was not.  The total protein lysates were digested via FASP and then subjected to LC-MS/MS without prior fractionation.  CPGR employed a Q-Exactive mass spectrometer on each sample, producing RAW files that span 130 minutes for each experiment.  The fragmentation method was \"HCD\" at a collision energy of 26, with high resolution measurements in both MS and MS/MS scans.  CPGR started with a test run of sample 9 (see \"_01\" on file name) but then ran each of the sample vials once afterward (it seems likely the initial 9 run was to test that methods were working appropriately).  After each of the vials had been run, CPGR opted to re-run sample tubes 2, 3, and 4 to produce the files with 'a' suffixed to the tube numbers.\n\nThe sample numbers may be understood by this legend:\nS1-S3\t   SAWC1129   SMX cohort (three replicates)\nS4-S6\t   SAWC1129   Control cohort (three replicates)\nS7-S9\t   K636\t   SMX cohort (three replicates)\nS10-S12\t   K636\t   Control cohort (three replicates)\n\nOnly the K636 data have been described in a manuscript, at this point.  We decided, however, to upload all the RAW data since they were produced as a single set.\n\nThe quality metrics were produced by the \"IDFree\" mode of QuaMeter, available as part of the \"Bumbershoot\" package at ProteoWizard.  The metrics, supplied in the \"metrics.tsv\" file, were produced using the configuration file provided as quameter.cfg.\n\nProteoWizard msConvert was used to produce centroided mzML files from the RAWs, using the \"zip\" option.  These spectra were identified in MS-GF+ 20170113 on a Linux machine using the sequence database \"20170518-Ensembl-Mycobacterium_tuberculosis_h37rv-Cntms.ASM19595v2.pep.all.fasta.\"  [NOTE: this search assumed an iodoacetamide artifact of Cys+57 rather than Cys+46.] The command-line options for running MS-GF+ are given in msgf.sh.  The mzID files were imported by IDPicker 3.1.9729, then filtered by requiring a PSM FDR of 0.01 and a minimum of three spectra overall per protein group.  An empirical protein FDR of 3.43% resulted, spanning 1559 discernible protein groups.  Individual RAW files were organized to an experimental hierarchy defined in Assemble.txt.  Identification results were exported in a spectral count per protein per experiment pivot table (20180326-IDPicker-Spectral-Counts-Per-Protein) and a spectral count per experiment table (20180326-IDPicker-Spectral-Count-Summary).  These identifications are different than the set featured in the corresponding publication; they are intended only to be illustrative of the sample content.\n","dates":{"publication":"Sun Mar 25 22:29:00 BST 2018"},"accession":"MSV000082209","cross_references":{"pubmed":["30029919"]}}