{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000082403/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Zsuzsanna Darula"],"instrument_platform":["LTQ Orbitrap Elite"],"species":["Human"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=75e85180ea3740daadd024e11f9401b8"],"submitter_email":["zdarula@brc.hu"],"submitter_affiliation":["Biological Research Centre"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["13"],"ptm_modification":["O-glycosylation"],"data_protocol":[""],"description_synonyms":["N Acylneuraminate Glycohydrolases, Oligosaccharide Sialidase, peptides, determination, Artocarpus lectin jacalin, Exo-alpha-Sialidase, Exo alpha Sialidase, chemical analysis., Glycopeptide, Acylneuraminyl hydrolase, N-Acylneuraminate, heavy chain disease, Peptide, N-Acylneuraminate Glycohydrolases, polypeptide, peptide, Polypeptides, Oligosaccharide, peptido, HCD, Peptid, peptidos, ETD, Polypeptide, assay, Glycohydrolases, Chromatographies, jackfruit lectin jacalin, Sialidase"],"name_synonyms":["Glycopeptide., jackfruit lectin jacalin, Artocarpus lectin jacalin"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Reproducibility of a WGA-Jacalin based affinity enrichment of O-glycopeptides from human serum tryptic digest","description":"Human serum tryptic digest was enriched on a WGA-POROS affinity column collecting two glycopeptide fractions. The fractions were treated with neuraminidase and O-glycopeptides were isolated by a subsequent Jacalin affinity chromatography. Peptide mixtures were analyzed by LC/MS-MS applying HCD fragment ion dependent ETD analysis using an Orbitrap Elite mass spectrometer. Technical reproducibility was assessed using 3 parallel samples.\n","dates":{"publication":"Fri May 25 07:24:00 BST 2018"},"accession":"MSV000082403","cross_references":{"TAXONOMY":["9606"]}}